1. ProductProfile
SureFIND Transcriptome PCR Array
For discovering regulators of gene expression
The SureFIND Transcriptome PCR Array identifies which miRNA, protein, or pathway regulates the expression of any gene.
In each well of a SureFIND Transcriptome PCR Array, is a unique “transcriptome” or cDNA sample synthesized from cells
treated with a unique miRNA mimic, siRNA, or chemical inhibitor. A single qPCR reaction screens 90 different regulators.
An expression change in a specific well reveals a transcriptional regulator for the tested gene.
The SureFIND Transcriptome PCR Array provides:
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Rapid discovery of gene expression regulators
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Pre-loaded arrays for simple setup
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Compatibility with SYBR® Green or hydrolysis probe assays
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Protocol overview
Included data analysis software
Prepare reaction
mix with genespecific assay
and master mix
Load SureFIND
Transcriptome
PCR Array with
reaction mix
Greater savings and unparalleled ease of use
SureFIND Transcriptome PCR Array layout
The SureFIND Transcriptome PCR Array is a single PCR plate with data
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synthesized from an experimentally treated cell sample. The cells are
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analysis software. Each well (Figure 1) has a unique cDNA sample
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treated with a panel of miRNA mimics, chemicals, or siRNAs. By
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correlation, a treatment that changes the expression of a gene is identified
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as a candidate regulator. Therefore, the SureFIND Transcriptome PCR
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Arrays allow you to screen 90 different regulators in a single assay.
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90 VTC VTC VTC VTC PPC PPC
To perform the assay, add a gene-specific qPCR assay to every well in
Preloaded cDNA samples
the array and run it in a qPCR instrument. SYBR Green and probe-based
Vehicletreated
controls
Positive
PCR
controls
qPCR assays are compatible with these arrays. CT values obtained
are imported into the software to identify regulators of the selected
1.E+001
calculations and delivers a list of which miRNA, transcription factor, or
pathway regulates the tested gene.
Delta Rn
gene’s expression. The software performs the ΔΔCT based fold-change
Thermocycling
run in qPCR
instrument
1.E-000
1.E-001
1.E-002
1.E-003
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Cycle number
Figure 1. Protocol overview. SureFIND Transcriptome PCR Arrays preloaded with cDNA
synthesized from 90 different experimentally treated cell samples. Each unique cDNA sample is
derived from cells treated with a miRNA mimic, chemical, or siRNA. After a qPCR reaction for a
gene of interest, the software identifies which miRNA, protein, or pathway regulates that gene.
Identify transcriptional regulators:
Export CT values to data analysis software
Sample & Assay Technologies
