Emt and ecm 2013

Sample & Assay Technologies

EMT and ECM

Jesse Liang, Ph.D.


Epithelial Cells

1. Closely adjoined
2. Polarized
Epithelial Markers:
E-Cadherin (adherens junctions)
Claudins (tight junctions)
Occludin (tight junctions)
Desmoplakin (desmosomes)
Cytokeratin-8, -18 and -19
Mucin-1
There are 5 different types of cell junctions. They are tight junctions,
adherens junctions, desmosomes, hemidesmosomes, and gap junctions.


Mesenchymal Cells

1. Not adjoined
2. No polarity
Mesenchymal Markers:
Vimentin
N-Cadherin
Fibronectin
Vitronectin
FSP1(fibroblast-specific protein 1)
Smooth-muscle actin
FGFR2 IIIb and IIIc splice variants


EMT (Epithelial-Mesenchymal Transition)

Epithelial cells can convert into mesenchymal cells by a process
known as EMT, which disrupts cell-cell adhesion and cell-ECM
adhesion.
Cell remodeling
+
ECM remodeling

* Embryogenesis and development
* Cancer
* Fibrosis


The Nature of EMT

1. transient and reversible
2. highly context-specific
3. a vicious cycle in pathological conditions


Epithelial-Mesenchymal Transitions
in Development and Disease
Cell (2009) 139, 871-890.

Activators and Suppressors of EMT

highly context-specific

TGFβ, FGF, EGF families, HGF; Src, GTPase family – Ras, Rho, Rac; Snail, Slug, Twist, ZEB, NFκB
Extracellular
Cytoplasmic
Nuclear


TGF-β and EMT & ECM

TGF-β is the best characterized and most often used
inducer of EMT. Other heavily involved signaling pathways
of EMT include PI3K-Akt, GPCR, MAPK, Wnt, Notch,
Hedgehog, NFκB, HIF, integrins, and growth factors.
TGF-β is also one of the key cytokines in regulating
ECM, not only by regulating expression of ECM structural
proteins, but also by affecting enzymes involved in ECM
biosynthesis and degradation.


Wilm’s tumor gene 1,
In heart development

EMT Signaling

Oxygen-dependent gene expression in
development and cancer: lessons learned
from the Wilm’s tumor gene, WT1.
Front Mol Neurosci (2011), 4:1-11.


EMT and Cancer

• Progression

of most carcinomas is associated with the
acquisition of mesenchymal phenotype.
• Cells with an EMT phenotype induced by different factors are
rich sources for cancer stem-like cells.
• Moreover, induction of EMT in tumor cells not only promotes
invasion and metastasis but also contributes to drug resistance.


Induction of EMT Generates Stem-Like Cells

Breast cancer – initiating cells are CD44+CD24- cells.
EMT phenotype

EMT induction

Mani SA, et al. The Epithelial-Mesenchymal Transition Generates Cells
with Properties of Stem Cells. Cell (2008), 133, 704-715.

Leukemia – initiating cells are CD34+CD38- cells.
Colon cancer – initiating cells are CD133+ cells.
Brain cancer – initiating cells are CD133+ cells.
Prostate cancer – initiating cells are CD44+α2β1+ cells.


miRNAs Link EMT to Stem-Like Cells in Human Cancers
miR-200 family and ZEB1/2

miR-200a
* Knockdown of Akt-1 decreases the expression of miR-200 family including miR200a, and increases mammosphere forming ability in breast cancer

miR-200b
* miR-200b inhibits expression of ZEB1, ZEB2, Lin28B and Notch1 in prostate
cancer
* miR-200b targets Suz12 and contributes to cancer stem cells maintenance in
breast cancer

miR-200c
* miR-200c inhibits expression of ZEB1, ZEB2 and Bmi1 in breast cancer;
* miR-200c inhibits expression of ZEB1, Sox2, Bmi1 and KLF4 in pancreatic
cancer

miR-183
* ZEB1 represses miR-183 expression, which increases the expression of Bmi1
and KLF4 in pancreatic cancer

miR-203
* ZEB1 represses miR-203 expression, which increases the expression of Bmi1
and KLF4 in pancreatic cancer


miR-200 Links EMT to ECM

• Several miRNAs have been identified as either oncogenes (miR-17–
92, miR-155, miR-21) or tumor suppressors (miR-15a, miR-16a, let-7)
and some human tumor types can be classified by miRNA signatures.
• The miR-200 family of miRNAs consists of five members (miR-200a,
200b, miR-200c, 141, 429) that have been demonstrated to have a
role in EMT through regulation with the ZEB transcription factors and
regulation of E-cadherin and vimentin expression.
• The most striking effect of miR-200 expression was a change in
protein constituents in the media resulting from protein secretion and
shedding with downregulation of extracellular matrix, peptidases and
cell adhesion proteins.
• Proteins upregulated with miR-200 restoration were associated
primarily with cytoskeletal regulation and cell adhesion.
- Cancer Research (2011) Dec 15; 71(24): 7670–7682


EMT and Fibrosis

Fibrosis is characterized by the presence of an excess of
fibrous connective tissue in an organ, and in particular by an
excessive deposition of collagen I.
Renal fibrosis, for example, has been associated with the
activation of interstitial fibroblasts, which give rise to collagen
secreting myofibroblasts. In addition, myofibroblasts can also
originate from renal tubular epithelial and endothelial cells that
undergo EMT.
Activation of Snail1, a well known EMT inducer, leads to renal
fibrosis and renal failure in animal models. High Snail1
expression and evidence of EMT has also been found in the
kidneys of patients with renal fibrosis (Boutet et al, 2006).


Cancer and Fibrosis are (Induced by) Inflammation

In the context of a chronic inflammatory condition, TGFβ1
and hypoxia activate EMT that converges in the activation of
NFκB, which is also induced by the inflammatory cytokines
and oxidative stress.


Inflammation, Oxidative Stress and Hypoxia in EMT Induction

Inflammation and EMT: an alliance
towards organ fibrosis and cancer
progression. EMBO Molecular
Medicine (2009), 1, 303-314.


PCR Array Introduction

84 Pathway-Specific
Genes of Interest
5 Housekeeping Genes
Genomic DNA
Contamination Control
Reverse Transcription
Controls (RTC) n=3
Positive PCR Controls
(PPC) n=3


EMT PCR Arrays

http://www.sabiosciences.com/rt_pcr_product/HTML/PAHS-090Z.html
Genes Up-Regulated During EMT:
AHNAK, BMP1, CALD1, CAMK2N1, CDH2, COL1A2, COL3A1, COL5A2, FN1, FOXC2, GNG11, GSC, IGFBP4, ITGA5, ITGAV,
MMP2, MMP3, MMP9, MSN, SERPINE1, SNAI1, SNAI2, SNAI3, SOX10, SPARC, STEAP1, TCF4, TIMP1, TMEFF1, TMEM132A,
TWIST1, VCAN, VIM, VPS13A, WNT5A, WNT5B.
Genes Down-Regulated During EMT:
CAV2, CDH1 (E-cadherin), DSP, FGFBP1, IL1RN, KRT19, MST1R, NUDT13, OCLN, PPPDE2, RGS2, SPP1 (Osteopontin), TFPI2,
TSPAN13.
Differentiation & Development:
AKT1, BMP1, BMP2, BMP7, COL3A1, COL5A2, CTNNB1, DSP, ERBB3, F11R, FOXC2, FZD7, GSC, JAG1, KRT14,
MST1R, NODAL, NOTCH1, PTP4A1, SMAD2, SNAI1, SNAI2, SOX10, TGFB2, TGFB3, TMEFF1, TWIST1, VCAN, WNT11, WNT5A,
WNT5B.
Morphogenesis: CTNNB1, FOXC2, JAG1, RAC1, SMAD2, SNAI1, SOX10, TGFB1, TGFB2, TGFB3, TWIST1, WNT11, WNT5A.
Cell Growth & Proliferation: AKT1, BMP1, BMP7, CAV2, CTNNB1, EGFR, ERBB3, FGFBP1, FOXC2, IGFBP4, ILK, JAG1, MST1R,
NODAL, PDGFRB, TGFB1, TGFB2, TGFB3, TIMP1, VCAN, ZEB1.
Migration & Motility: CALD1, CAV2, EGFR, FN1, ITGB1, JAG1, MSN, MST1R, NODAL, PDGFRB, RAC1, STAT3, TGFB1, VIM.
Cytoskeleton: CAV2, KRT7, MAP1B, PLEK2, RAC1, VIM.
Extracellular Matrix & Cell Adhesion: BMP1, BMP7, CDH1 (E-cadherin), CDH2 (N-cadherin), COL1A2, COL3A1, COL5A2,
CTNNB1, DSC2, EGFR, ERBB3, F11R, FN1, FOXC2, ILK, ITGA5, ITGAV, ITGB1, MMP2, MMP3, MMP9, PTK2, RAC1, SERPINE1
(PAI-1), SPP1 (Osteopontin), TGFB1, TGFB2, TIMP1, VCAN.
Signaling Pathways:
Estrogen Receptor: CAV2, ESR1 (ERa), KRT19, TGFB3.
G-Protein Coupled Receptor: AKT1, FZD7, GNG11, RAC1, RGS2.
Integrin-Mediated: COL3A1, ILK, ITGA5, ITGAV, ITGB1, PTK2.
Notch: FOXC2, JAG1, NOTCH1.
Receptor Tyrosine Kinase: EGFR, ERBB3, PDGFRB, RGS2, SPARC.
TGFß / BMP: BMP1, BMP2, BMP7, COL3A1, SMAD2, TGFB1, TGFB2, TGFB3.
WNT: CTNNB1, FZD7, GSK3B, WNT11, WNT5A, WNT5B.
Transcription Factors: CTNNB1, ESR1 (ERa), FOXC2, GSC, NOTCH1, SIP1, SMAD2, SNAI2, SNAI3, SOX10, STAT3, TCF3, TCF4,
TWIST1, ZEB1, ZEB2.


EMT Methylation PCR Arrays

http://www.sabiosciences.com/dna_methylation_product/HTML/EAHS-901Z.html
Genes Down-Regulated During EMT:
CDH1, DSP, KRT19, MST1R, OCLN, PPPDE2, RGS2, TSPAN13.
Differentiation & Development: MST1R, PTP4A1, SMAD4.
Cell Growth & Proliferation: GAB1, MST1R, SEH1L, SMAD4.
Extracellular Matrix & Cell Adhesion: CDH1, CTNNAL1, DSC2, EPCAM, NID2.
Signal Transduction: GAB1, KRT19, MAP3K5, RGS2, SMAD4, TGIF1.
Cytoskeleton: CTNNAL1, KRT7, PLEK2.
Other Genes: PLSCR1, YES1.


EMT ChIP PCR Arrays – Profile Histone Codes

http://www.sabiosciences.com/chipqpcr_product/HTML/GH-090A.html
Genes Up-Regulated During EMT: AHNAK, BMP1, CALD1, CDH2 (N-cadherin), COL1A2, COL3A1, COL5A2, FN1, FOXC2, GNG11, GSC, IGFBP4, ITGA5,
ITGAV, MMP2 (Gelatinase A), MMP3, MMP9 (Gelatinase B), MSN, SERPINE1 (PAI-1), SNAI1, SNAI2, SNAI3, SOX10, SPARC, STEAP1, TCF4, TIMP1,
TMEFF1, TMEM132A, TWIST1, VCAN, VIM, VPS13A, WNT5A, WNT5B.
Genes Down-Regulated During EMT: CAV2, CDH1 (E-cadherin), DSP, FGFBP1, IL1RN, KRT19, MITF, MST1R, NUDT13, PPPDE2, RGS2, SPP1
(Osteopontin), TFPI2, TSPAN13.
Genes with Known Histone Modifications during EMT:
Increased H3K4me3: AHNAK, AKT1, BMP1, CALD1, CAV2, CDH2 (N-cadherin), CTNNB1, FN1, FZD7, GNG11, GSK3B, IGFBP4, ILK, ITGA5, MAP1B, MITF,
MMP2 (Gelatinase A), RGS2, SERPINE1 (PAI-1), SNAI1, SNAI2, SPARC, TCF4, TGFB1, TGFB2, TGFB3, TIMP1, TMEFF1, TSPAN13, VIM, VPS13A,
WNT5A.
Decreased H3K27me3: DSP, FGFBP1, GSC, IL1RN.
Differentiation & Development: AKT1, BMP1, BMP2, BMP7, COL3A1, COL5A2, CTNNB1, DSP, ERBB3, F11R, FOXC2, FZD7, GSC, KRT14, MITF, MST1R,
NODAL, NOTCH1, PTP4A1, SMAD2, SNAI1, SNAI2, SOX10, TGFB2, TGFB3, TMEFF1, TWIST1, VCAN, WNT11, WNT5A, WNT5B.
Morphogenesis: CTNNB1, FOXC2, PPP3R1, RAC1, SMAD2, SNAI1, SOX10, TGFB1, TGFB2, TGFB3, TWIST1, WNT11, WNT5A.
Cell Growth & Proliferation: AKT1, BMP1, BMP2, BMP7, CAV2, CTNNB1, EGFR, ERBB3, FGFBP1, FOXC2, HIF1A, IGFBP4, ILK, MST1R, NODAL, PDGFRB,
TGFB1, TGFB2, TGFB3, TIMP1, VCAN.
Migration & Motility: CALD1, CAV2, EGFR, FN1, ITGB1, MSN, MST1R, NODAL, PDGFRB, RAC1, STAT3, TGFB1, VIM.
Cytoskeleton: CAV2, KRT7, MAP1B, PLEK2, RAC1, VIM.
Extracellular Matrix & Cell Adhesion: BMP1, BMP2, BMP7, CDH1 (E-cadherin), CDH2 (N-cadherin), COL1A2, COL3A1, COL5A2, CTGF, CTNNB1, DSC2,
EGFR, ERBB3, F11R, FN1, FOXC2, ILK, ITGA5, ITGAV, ITGB1, MMP2 (Gelatinase A), MMP3, MMP9 (Gelatinase B), PTK2, RAC1, SERPINE1 (PAI-1), SPP1
(Osteopontin), TGFB1, TGFB2, TIMP1, VCAN.
Signaling Pathways:
Estrogen Receptor: CAV2, ESR1 (ERa), KRT19, TGFB3.
G-Protein Coupled Receptor: AKT1, FZD7, GNG11, RAC1, RGS2.
Integrin-Mediated: COL3A1, CTGF, ILK, ITGA5, ITGAV, ITGB1, PTK2.
Notch: FOXC2, NOTCH1.
Receptor Tyrosine Kinase: EGFR, ERBB3, PDGFRB, RGS2, SPARC.
TGFb / BMP: BMP1, BMP2, BMP7, COL3A1, SMAD2, SMAD4, TGFB1, TGFB2, TGFB3.
WNT: CTNNB1, FZD7, GSK3B, WNT11, WNT5A, WNT5B.

.

Transcription Factors: CTNNB1, ESR1 (ERa), FOXC2, GSC, MITF, NOTCH1, SIP1, SMAD2, SNAI2, SNAI3, SOX10, STAT3, TCF4, TWIST1, ZEB2


miScript miRNA PCR Arrays

http://www.sabiosciences.com/mirna_pcr_array.php
miRNome (miRBase V19) Human Mouse Rat Dog Rhesus Macaque
miRNome (miRBase V19) 384 Well Human Mouse Rat Dog Rhesus
Macaque
miFinder 96 Well Human Mouse Rat Dog Rhesus Macaque
miFinder 384 Well Human Mouse
Apoptosis Human Mouse Rat
Brain Cancer Human Mouse Rat
Breast Cancer Human Mouse Rat
Cancer PathwayFinder Human Mouse Rat
Cardiovascular Disease Human Mouse Rat
Diabetes Human Mouse Rat
Cell Differentiation & Development Human Mouse Rat
Immunopathology Human Mouse Rat
Inflammatory Response and Autoimmunity Human Mouse Rat
Neurological Development & Disease Human Mouse Rat
Ovarian Cancer Human Mouse Rat
Prostate Cancer Human Mouse Rat
Serum & Plasma Human Mouse Rat
Serum & Plasma 384 Well Human
T-Cell & B-Cell Activation Human Mouse Rat
miRNA QC Human Mouse Rat Dog Rhesus Macaque


miRNA Targets PCR Arrays

After miRNA expression analysis with miScript miRNA PCR Arrays, perform the
following functional miRNA study.
Once you have treated your cells with a microRNA mimic, inhibitor, or target
protector, analyze target gene expression with the RT² Profiler miRNA Targets
PCR Arrays.
http://www.sabiosciences.com/miRNATargetsPCRArrays.php

A miRNA target gene should meet the following criteria:
Target gene expression inversely correlates with miRNA expression
Transfection with a miScript miRNA mimic downregulates target gene expression
Transfection with a miScript miRNA inhibitor re-activates target gene expression
Transfection with a miScript target protector re-activates target gene expression


ECM (Extracellular Matrix) and Cell Adhesion PCR Arrays

Cell Adhesion Molecules:
Transmembrane Molecules: CD44, CDH1, HAS1, ICAM1, ITGA1, ITGA2, ITGA3, ITGA4, ITGA5, ITGA6, ITGA7, ITGA8, ITGAL,
ITGAM, ITGAV, ITGB1, ITGB2, ITGB3, ITGB4, ITGB5, MMP14, MMP15, MMP16, NCAM1, PECAM1, SELE, SELL, SELP,
SGCE, SPG7, VCAM1.
Cell-Cell Adhesion: CD44, CDH1, COL11A1, COL14A1, COL6A2, CTNND1, ICAM1, ITGA8, VCAM1.
Cell-Matrix Adhesion: ADAMTS13, CD44, ITGA1, ITGA2, ITGA3, ITGA4, ITGA5, ITGA6, ITGA7, ITGA8, ITGAL, ITGAM, ITGAV,
ITGB1, ITGB2, ITGB3, ITGB4, ITGB5, SGCE, SPP1, THBS3.
Other Adhesion Molecules: CNTN1, COL12A1, COL15A1, COL16A1, COL5A1, COL6A1, COL7A1, COL8A1, VCAN, CTGF,
CTNNA1, CTNNB1, CTNND2, FN1, KAL1, LAMA1, LAMA2, LAMA3, LAMB1, LAMB3, LAMC1, THBS1, THBS2, CLEC3B, TNC,
VTN.
Extracellular Matrix Proteins:
Basement Membrane Constituents: COL4A2, COL7A1, LAMA1, LAMA2, LAMA3, LAMB1, LAMB3, LAMC1, SPARC.
Collagens & ECM Structural Constituents: COL11A1, COL12A1, COL14A1, COL15A1, COL16A1, COL1A1, COL4A2, COL5A1,
COL6A1, COL6A2, COL7A1, COL8A1, FN1, KAL1.
ECM Proteases: ADAMTS1, ADAMTS13, ADAMTS8, MMP1, MMP10, MMP11, MMP12, MMP13, MMP14, MMP15, MMP16,
MMP2, MMP3, MMP7, MMP8, MMP9, SPG7, TIMP1.
ECM Protease Inhibitors: COL7A1, KAL1, THBS1, TIMP1, TIMP2, TIMP3.
Other ECM Molecules: VCAN, CTGF, ECM1, HAS1, SPP1, TGFBI, THBS2, THBS3, CLEC3B, TNC, VTN.
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Tsau C, Ito M, Gromova A, Hoffman MP, Meech R, Makarenkova HP.
Barx2 and Fgf10 regulate ocular glands branching morphogenesis by
controlling extracellular matrix remodeling. Development (2011)
Aug;138(15):3307-17


Cell Junctions PCR Arrays

There are 5 different types of cell junctions. They are tight junctions,
adherens junctions, desmosomes, hemidesmosomes, and gap junctions.

Focal Adhesions

Tight Junctions

Adherens Junctions

Gap Junctions


Cancer Metastasis / Cell Motility PCR Arrays

Cancer Metastasis PCR Array:

Cell Motility PCR Array:

----------------------------------------------------------------------------------------------------------Zhu W, Cai MY, Tong ZT, Dong SS, Mai SJ, Liao YJ, Bian XW, Lin MC, Kung HF,
Zeng YX, Guan XY, Xie D. Overexpression of EIF5A2 promotes colorectal
carcinoma cell aggressiveness by upregulating MTA1 through C-myc to induce
epithelial-mesenchymal transition. Gut (2012) Apr;61(4): 562-575.


Fibrosis PCR Arrays

Pro-Fibrotic: ACTA2 (a-SMA), AGT, CCL11 (Eotaxin), CCL2 (MCP-1), CCL3 (MIP-1a), CTGF, GREM1, IL13,
IL13RA2, IL4, IL5, SNAI1 (Snail).
Anti-Fibrotic: BMP7, HGF, IFNG, IL10, IL13RA2.
Extracellular Matrix & Cell Adhesion:
ECM Components: COL1A2, COL3A1.
Remodeling Enzymes: LOX, MMP1 (Collagenase 1), MMP13, MMP14, MMP2 (Gelatinase A), MMP3, MMP8, MMP9
(Gelatinase B), PLAT (tPA), PLAU (uPA), PLG, SERPINA1 (a1-antitrypsin), SERPINE1 (PAI-1), SERPINH1, TIMP1,
TIMP2, TIMP3, TIMP4.
Cellular Adhesion: ITGA1, ITGA2, ITGA3, ITGAV, ITGB1, ITGB3, ITGB5, ITGB6, ITGB8.
Inflammatory Cytokines & Chemokines: CCL11 (Eotaxin), CCL2 (MCP-1), CCL3 (MIP-1a), CCR2, CXCR4, IFNG,
IL10, IL13, IL13RA2, IL1A, IL1B, IL4, IL5, ILK, TNF.
Growth Factors: AGT, CTGF, EDN1, EGF, HGF, PDGFA, PDGFB, VEGFA.
Signal Transduction:
TGFß Superfamily: BMP7, CAV1, DCN, ENG (EVI-1), GREM1, INHBE, LTBP1, SMAD2, SMAD3, SMAD4, SMAD6,
SMAD7, TGFB1, TGFB2, TGFB3, TGFBR1 (ALK5), TGFBR2, TGIF1, THBS1, THBS2
Transcription Factors: CEBPB, JUN, MYC, NFKB1, SP1, STAT1, STAT6
Epithelial-to-Mesenchymal Transition: AKT1, BMP7, COL1A2, COL3A1, ILK, ITGAV, ITGB1, MMP2 (Gelatinase
A), MMP3, MMP9, SERPINE1 (PAI-1), SMAD2, SNAI1 (Snail), TGFB1, TGFB2, TGFB3, TIMP1.
Others: BCL2, FASLG (TNFSF6).


Oxidative Stress PCR Arrays

Antioxidants:
Glutathione Peroxidases (GPx): GPX1, GPX2, GPX3, GPX4, GPX5, GPX6, GPX7, GSTP1, GSTZ1.
Peroxiredoxins (TPx): PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, PRDX6 (AOP2).
Other Peroxidases: CAT, CYBB, CYGB, DUOX1, DUOX2, EPX, LPO, MGST3, MPO, PTGS1, PTGS2 (COX2), PXDN, TPO,
TTN.
Other Antioxidants: ALB, APOE, GSR, MT3, SELS, SOD1, SOD3, SRXN1, TXNRD1, TXNRD2.
Genes Involved in Reactive Oxygen Species (ROS) Metabolism:
Superoxide Dismutases (SOD): SOD1, SOD2, SOD3.
Other Genes Involved in Superoxide Metabolism: ALOX12, CCS, DUOX1, DUOX2, GTF2I, MT3, NCF1, NCF2, NOS2 (iNOS),
NOX4, NOX5, PREX1, UCP2.
Other Genes Involved in ROS Metabolism: AOX1, BNIP3, EPHX2, MPV17, SFTPD.
Oxidative Stress Responsive Genes: APOE, ATOX1, CAT, CCL5 (RANTES), CYGB, DHCR24, DUOX1, DUOX2, DUSP1
(PTPN16), EPX, FOXM1, FTH1, GCLC, GCLM, GPX1, GPX2, GPX3, GPX4, GPX5, GPX6, GPX7, GSR, GSS, HMOX1,
HSPA1A, KRT1, LPO, MBL2, MPO, MSRA, NQO1, NUDT1, OXR1, OXSR1, PDLIM1, PNKP, PRDX2, PRDX5, PRDX6
(AOP2), PRNP, RNF7, SCARA3, SELS, SEPP1, SIRT2, SOD1, SOD2, SQSTM1, SRXN1, STK25, TPO, TTN, TXN, TXNRD1,
TXNRD2.
Oxygen Transporters: CYGB, MB.
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------

Joyce NC, Harris DL, Zhu CC. Age-related gene response of human
corneal endothelium to oxidative stress and DNA damage. Invest
Ophthalmol Vis Sci (2011) Mar 1;52(3):1641-9


Hypoxia PCR Arrays

HIF1 & Co-Transcription Factors: ARNT, COPS5, HIF1A, HIF1AN, HIF3A, HNF4A, NCOA1, PER1.
Other HIF1 Interactors: APEX1, EGLN1, EGLN2, NFKB1, P4HA1, P4HB, TP53.
Responsive Genes:
Angiogenesis: ADORA2B, ANGPTL4, ANXA2, BTG1, EGR1, EDN1, EPO, F3, GPI, HMOX1, JMJD6, LOX, MMP9, PGF,
PLAU (uPA), SERPINE1 (PAI-1), VEGFA.
Coagulation: ALDOA, ANXA2, F10, F3, F3, PLAU (uPA), SERPINE1 (PAI-1), SLC16A3.
DNA Damage Signaling & Repair: ATR, MIF, NDRG1, RUVBL2.
Metabolism: ALDOA, DDIT4 (REDD1), ENO1, ERO1L, GBE1, GPI, GYS1, HK2, LDHA, PDK1, PFKFB3, PFKFB4, PFKL,
PFKP, PGAM1, PGK1, PKM2, SLC2A1, SLC2A3, TPI1.
Regulation of Apoptosis: ADM, BNIP3, BNIP3L, BTG1, DDIT4 (REDD1), IER3, MIF, NOS3 (eNOS), PIM1.
Regulation of Cell Proliferation: ADM, BTG1, BLM, CCNG2, EGR1, IGFBP3, MET, MIF, MXI1, NAMPT, NOS3 (eNOS),
ODC1, PGF, PIM1, TXNIP.
Transcription Factors: BHLHE40, FOS, RBPJ, USF2.
Transporters, Channels & Receptors: SLC2A1, SLC2A3, SLC16A3, TFRC, VDAC1.
Other Responsive Genes: ANKRD37, CA9, CTSA, DNAJC5, EIF4EBP1, LGALS3, MAP3K1.
-------------------------------------------------------------------------------------------------------------------------------------------------------------------

Mueller BR, Bale TL. Sex-specific programming of offspring emotionality
after stress early in pregnancy. J Neurosci (2008) Sep 3;28(36):9055-65


NFκB Signaling or Targets PCR Arrays

NFκB Signaling Pathway Plus PCR Array:
http://www.sabiosciences.com/rt_pcr_product/HTML/PAHS-025Y.html

NFκB Signaling Pathway PCR Array:

NFκB Signaling Targets PCR Array:
-------------------------------------------------------------------------------------------------------------

Fiume G, Vecchio E, De Laurentiis A, Trimboli F, Palmieri C, Pisano A,
Falcone C, Pontoriero M, Rossi A, Scialdone A, Fasanella Masci F, Scala
G, Quinto I. Human immunodeficiency virus-1 Tat activates NF-kappaB via
physical interaction with IkappaB-alpha and p65. Nucleic Acids Res. 2011
Dec 19.


TGFβ Signaling or Targets PCR Arrays

TGFβ / BMP Signaling Pathway Plus PCR Array:

TGFβ / BMP Signaling Pathway PCR Array:

TGFβ Signaling Targets PCR Array:
----------------------------------------------------------------------------------------------------------------

Garamszegi N, Garamszegi SP, Shehadeh LA, Scully SP.
Extracellular matrix-induced gene expression in human breast cancer cells.
Mol Cancer Res. (2009) Mar;7(3):319-29


Wnt Signaling or Targets PCR Arrays

Wnt Signaling Pathway Plus PCR Array:

Wnt Signaling Pathway PCR Array:

Wnt Signaling Targets PCR Array:
----------------------------------------------------------------------------------------------------------------

Burkhalter RJ, Symowicz J, Hudson LG, Gottardi CJ, Stack MS. Integrin
regulation of beta-catenin signaling in ovarian carcinoma. J Biol Chem.
(2011) Jul 1;286(26):23467-75.
Hussain M, Rao M, Humphries AE, Hong JA, Liu F, Yang M, Caragacianu D,
Schrump DS. Tobacco smoke induces polycomb-mediated repression of
Dickkopf-1 in lung cancer cells. Cancer Res. (2009) Apr 15;69(8):3570-8.


Contact Information

Jesse Liang
Email: jesse.liang@qiagen.com
Technical Support: 1-888-503-3187
Email: support@sabiosciences.com
Check Webinar Calendar:
http://www.sabiosciences.com/seminarlist.php
miRNA PCR Arrays: April 10, 17, 24 (Wednesdays)
Mutation PCR Arrays: April 9 (Tuesday)
Next Generation Sequencing (NGS): April 4,11,18 (Thursdays)
Copy Number Variation PCR Arrays: April 25 (Thursday)

Emt and ecm 2013

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