1. PRACTICAL REVISION

2. Scheme for slides
With background Without background
Brown Blue Red
Organism Gram +ve
cocci
Organism Gram -ve

3. Schemes for slides
With background Without background
Gram positive cocci Gram positive bacilli
Gram
negative
AND MIXTURES

4. Staphylococci in culture

5. Staphylococci in culture

6. Staphylococci in pus

7. Staphylococci in Pus

8. Streptococci in culture

9. Streptococci in culture

10. Streptococci in Pus

11. Streptococci in pus

12. S. pyogenes
Streptococci in pus

13. Pneumococci in tissue

14. Pneumococci in tissue

15. Pneumococci in tissue

16. Quellung reaction
(Streptococcus pneumoniae)

17. Neisseria in culture

18. Pathogenic Neisseria in pus

19. Pathogenic Neisseria in pus

20. Pathogenic Neisseria in pus

21. Pathogenic Neisseria in pus

22. Corynebacterium diphtheriae
(Gram stain)

23. Corynebacterium diphtheriae
(M.B. stain)

24. Corynebacterium diphtheriae
(M.B. stain)

25. Diphtheroids

26. Bacillus anthracis
(Gram stain)

27. Bacillus anthracis
(Gram stain)

28. B. anthracis
(Spore stain)

29. B. anthracis
(Spore stain)

30. B. anthracis
McFadyean reaction(polychrome MB stain)

31. Clostridium tetani

32. Clostridium tetani

33. Mycobacterium tuberculosis
(Acid Fast bacilli Z-N stain)

34. Mycobacterium tuberculosis
(Acid Fast bacilli Z-N stain)

35. Mycobacterium tuberculosis
(Acid Fast bacilli Z-N stain)

36. Mycobacterium leprae (Acid Fast
bacilli with modified Z-N stain)

37. Mycobacterium leprae
(Acid Fast bacilli with modified Z-N
stain)

38. Gram negative bacilli

39. Klebsiella in Tissues

40. Vibrio

41. Vibrio

42. Vibrio

43. Gram negative bacilli & Staphylococci
Mixture

44. Gram negative bacilli & Staphylococci
Mixture

45. Spirochaetes (Dark-field microscopy)

46. Spirochaetes (Fontana stain)

47. Spirochaetes
(Fontana stain)

48. Spirochaetes (fontana stain)

49. Vincent’s angina

50. Candida

51. Candida

52. Mixture: Candida & Gram –ve bacilli

53. Mixture: Candida & Gram –ve bacilli

54. C. albicans
(Positive Germ Tube test)

55. C. albicans
(Positive Germ Tube test)

56. Nutrient agar

57. Staphylococcus aureus on nutrient agar
( showing golden yellow endopigment )

58. Staphylococcus aureus on nutrient agar
( showing golden yellow endopigment )

59. Pseudomonas aeruginosa on nutrient
agar(showing greenish exopigment)

60. Proteus on nutrient agar
(showing swarming growth)

61. Proteus on nutrient agar
(showing swarming growth)

62. Mannitol salt agar

63. Mannitol salt agar

64. Mannitol salt agar

65. Blood agar

66. Types of haemolysis on Blood agar

67. Alpha-haemolysis on blood agar

68. Beta-haemolysis on blood agar

69. No-haemolysis on blood agar

70. Bacitracin test
Streptococi other than
group A

71. Bacitracin test
Streptococci
other than group A
(Resistant)
group A streptococci
(Sensitive)

72. Optochin test

73. Optochin test
Sensitive
(Pneumococci)
Resistant
(Viridans streptococci)

74. Optochin test (sensitive)
Pneumococci

75. Optochin test (resistant)
Viridans streptococci

76. Corynebacterium diphtheriae on Blood
Tellurite

77. Corynebacterium diphtheriae
on Blood Tellurite

78. Loffler’s serum
for C. diphtheriae

79. Chocolate agar
for Neisseria and Haemophilus

80. Haemophilus or Neisseria on
chocolate agar

81. Haemophilus influenzae on blood agar showing
satellatism
(growth around staphylococcus culture)

82. Haemophilus influenzae
(growth only around both factors X & V)
xv

83. Lowenstein Jensen medium
for M. tuberculosis

84. Mycobacterium tuberculosis on
Lowenstein Jensen medium

85. M. tuberculosis on L.J. medium

86. Mycobacterium Growth Indicator Tube
(MGIT) (Positive tubes 1, 5 & 6)
1
2 3 4 5 6

87. Mycobacterium Growth Indicator
Tube (MGIT)
Positive
fluorescenceNegative
fluorescence

88. MacConkey’s medium

89. Lactose & Non-lactose fermenter on
MacConkey’s medium

90. Lactose fermenter on MacConkey’s
medium

91. Lactose fermenters on
MacConkey’s medium

92. Klebsiella (mucoid & LF) on
MacConkey’s medium

93. Lactose non-fermenter on MacConkey’s
medium

94. Lactose non-fermenter on
MacConkey’s medium

95. LNF on MacConkey’s medium

96. Salmonella on
Salmonella-Shigella (S-S) agar

97. T.C.B.S. medium

98. Vibrio cholerae on T.C.B.S.

99. Blood culture technique
1
2
35 – 10 ml blood
Added to
50 – 100 ml
fluid medium
Subculture on
Solid medium

100. Candle Jar ( 5 – 10% CO2)

101. Candle Jar ( 5 – 10% CO2)

102. Cooked meat medium
for anaerobic cultivation

103. Anaerobic Jar

104. Anaerobic Jar

105. Triple sugar iron( TSI )
TSI
Lactose
Fermenter + gas
e.g. E. coli,
Klebsiella
Glucose
Fermenter
e.g.Shigella,
Salmonella
Glucose
Fermenter
+ H2S
e.g.Salmonella
Non-fermenter
e.g.
Pseudomonas

106. TSI
Lf +gas
e.g.
E.coli,Kleb
NL
e.g.
Ps
TSI ggGl fer
e.g.Sal,
Shig
gl-ferm+H2S
e.g.
Salmonella

107. TSI
Non fermenter
e.g.
Pseudomonas
Lactose
ferm + gas
e.g. E. coli,
Klebsiella
Glucose
fermenter
e.g.Shigella,
Salmonella
Glucose
fermenter
+ H2S
e.g.Salmonella
Glucose
Fermenter
+H2S
e.g.Salmonella

108. Urease test
-ve +ve
e.g. Proteus

109. Catalase Test
e.g
Staphylococci
e.g.
Streptococci

110. Catalase Test
e.g
Staphylococci
e.g.
Streptococci

111. Coagulase Test
Staphylococcus aureus
Coagulase negative
staphylococci,
e.g. S. epidermidis

112. Coagulase test
Staphylococcus aureus Coagulase negative staphylococci,
e.g. S. epidermidis

113. Oxidase test
Oxidase positive
e.g. Neisseria, Pseudomonas

114. Oxidase test
Oxidase positive
e.g. Neisseria,
Pseudomonas

115. Oxidase test
+ ve - ve
Oxidase positive
e.g. Neisseria,
Pseudomonas

116. Indole test
- ve
e.g. Klebsiella
+ ve
e.g. E.coli

117. MR & VP tests
MR test VP test
-ve +ve

118. Citrate test

119. B.R. of E. coli
I M V C + + - - TSI
MR VPIndole Citrate

120. B.R. of Klebsiella
I M V C - - + + TSI
VP

121. Nagler’s Reaction
(Clostridium perfringens)
Antitoxin No antitoxin

122. Nagler’s Reaction
(Clostridium perfringens)

123. Analytical Profile Index (API)
Biochemical identification system

124. Analytical Profile Index (API)
Biochemical identification system

125. Analytical Profile Index (API)
Biochemical identification system

126. String Test (Positive in Vibrio cholerae)

127. String Test (Positive in Vibrio cholerae)

128. Tube agglutination test
(Titre:1/320)
TITRE
1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control

129. Brucella tube agglutination test
showing prozone phenomenon
(Titre:1/320)
TITRE
1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control

130. Widal Test (Recent infection with S. typhi)
(Titre:1/320)
O
H
C
T
C
PA
C
PB
C
1/20 1/40 1/80 1/160 1/320 1/640 1/1280
H
H

131. Widal Test (Old infection with S. typhi)
O
H
C
T
C
PA
C
PB
C
1/20 1/40 1/80 1/160 1/320 1/640 1/1280
H
H

132. Widal Test (Post vaccination)
O
H
C
T
C
PA
C
PB
C
1/20 1/40 1/80 1/160 1/320 1/640 1/1280
H
H

133. Latex Agglutination Test (A: Positive)

134. Latex agglutination Test (2 & 4: Positive )

135. Single radial immunodiffusion

136. Single radial immunodiffusion

137. Elek’s Test
Toxigenic C. diphtheriae Non-toxigenic C. diphtheriae

138. Elek’s Test

139. Immunofluorescence

140. Immunofluorescence

141. ELISA plate

142. ELISA plate

143. Tuberculin Skin Test (TST)
Positive

144. Antibiotic sensitivity (Disc diffusion)
1
2
3
6
5
4
The organism is
sensitive to 4, 5 & 6
Resistant to 1, 2 & 3

145. Antibiotic sensitivity (Disc diffusion)
Sensitive to…………
Resistant to………..

146. E-Test (MIC determination)

147. E-Test (MIC determination)

148. Phage-typing
1 2
3 4 5 6
7 8 9 10
11 12 13 14
15 16 17 18
19 20
The organism is sensitive to phages 4, 5, 6,……..etc.

149. Gel Electrophoresis
DNA
Marker
1 2 3 4 5 6

150. Gel Electrophoresis

151. Gel Electrophoresis

152. Western Blot
For diagnosis of HIV

153. Sterile Cotton Swab

154. Sterile cup
for collection of urine & sputum specimens

155. Sterile Gloves
For surgery & invasive procedures

156. Non- Sterile Gloves
For skin protection against blood & body fluids

157. Heavy-Duty Gloves
For environmental cleaning

158. Standard mask
Used for protection against droplet infections

159. N95 mask
Used for protection against airborne infections
e.g, M.tuberculosis

160. For protection against splashes of blood or body fluids
Aprons and Gowns

161. Eye Goggles
For protection against splashes of blood or body fluids

162. Rota virus (wheel-like appearance)

163. Rabies virus (bullet-shaped)

164. Coronavirus
(crown-like surface projection)

165. Tissue culture bottle
for cultivation of viruses

166. Plaques in a cell-monolayer

167. Examples of data show Exams

168. Choose a test

169. Choose and name the test used
to detect microbial antigen.
a
b
c d
a: Slide agglutination test

170. Choose a medium

171. Choose & name the selective medium used for
isolation of Mycobacterium tuberculosis.
(A)
(B)
(C)
(D)
D: Lowenstein-Jensen medium

172. Infection Control

173. Infection control
The most important PPE
when dealing with patients
having diarrhoea is:
173
a.
c.
b.
d.
d: Non-sterile gloves

174. Final
Practical
Examination

175. Final Practical Examination
NO Mobile Phones
are allowed in the department
(waiting rooms & exam. halls)

176. Practical exam. : Total 43 marks
Composed of 2 parts:
• Part 1: 13 marks
• Part 2: 30 marks

177. Part 1:
Demonstration:
Data show (total 13 Marks)
Formed of:
 3 different photos (3 marks/ 1 mark each)
ANY photo included in the modified revision CD
(version 2014)
 2 Choose a test
 2 Choose a medium
 1 Infection control
 5 Photos

178. Part 2:
Demonstrations (total 30 Marks)
Formed of 15 different stations (2 marks each)
In case of
a) microscopical slides: mention organism + stain
b) plain culture media: mention name + use
c) inoculated culture media: mention name +
the organism/effect
d) biochemical reactions: mention result + organism
e) serological test: mention name + result/titer
f) others: mention name + reading (e.g. phage-typing)

179. Name: Date:
No:
Microbiology Practical Exam
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30 Marks (2 marks each)