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Dr. Elliot Ryser Department of Food Science and Human Nutrition Michigan State University East Lansing, MI  September 14, 2011
 
 
 
Listeriosis Outbreaks Traced to Delicatessen-Sliced Turkey ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
Predicted Annual Listeriosis Cases (Log  Scale) from Different Food Categories in  the United States (FDA/FSIS, 2003) DM = Deli meats ;  FNR = Frankfurters (not reheated); P= Pâté and Meat Spreads; UM=  Unpasteurized Fluid Milk; SS= Smoked Seafood; CR = Cooked Ready-To-Eat  Crustaceans; HFD = High Fat and Other Dairy Products; SUC = Soft Unripened  Cheese; PM = Pasteurized Fluid Milk; FSC = Fresh Soft Cheese; FR = Frankfurters  (reheated); PF = Preserved Fish; RS = Raw Seafood; F = Fruits; DFS= Dry/Semi-dry  Fermented Sausages; SSC = Semi-soft Cheese; SRC = Soft Ripened Cheese; V =  Vegetables; DS = Deli-type Salads; IC= Ice Cream and Frozen Dairy Products; PC =  Processed Cheese; CD = Cultured Milk Products; HC = Hard Cheese.
USDA  Listeria  Alternatives Implemented October 2003
Deli  Meat Recalls, 1994 - 2005 0 2 4 6 8 10 12 1994 1995 1996 1997 1999 2000 2001 2002 2003 2004 2005 2006 Year Number of recalls Ham Turkey Chicken Beef Corned Beef Other > 84 recalls > 53 million lbs
Year Number of Recalls Recalled Product (lbs) 2006 1 375 2007 3 8600 2008 7 16,000 2009 3 39,000 2010 6 170,000 2011 6 9,500
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Current Concerns  ~ 1,600 listeriosis cases annually   including ~255 fatalities  ~ 290 of 1,600 listeriosis cases from deli  meats: ~  42 pre-sliced , ~  248 deli-sliced  ~  46 fatalities from deli meats  ~  6 fatalities from pre-sliced  ~  40 fatalities from deli-sliced
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Contamination primarily occurs after processing
Distribution of  Listeria  spp. in  a Local Deli/Restaurant ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Location Listeria L. seeligeri L. innocua L. mono ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Basement 35 20 7 8 Kitchen 50 27 11 12 Front line 7 7 0 0 Sandwich line 20 9 8 3 Seafood case 5 0 5 0 Cheese case 9 4 5 0 Meat case 7 4 3 0 Display floor 10 3 2 5 Open back case 1 1 0 0 Board (meat, cheese) 10 1 9 0 Slicer  (Hobart, sandwich line) 8 7 0 1 Slicer  (Bizerba, deli) 5 4 0 1 Slicer  (Bizerba, cheese) 2 2 0 0 Slicer  ( Hobart, basement sandwich line ) 0 0 0 0 Slicer  (Hobart), basement, cheese 1 1 0 0 ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Total 170 90 50 30 ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
_________________________________________________ Location Listeria  pos/ # of positive visits   # tested (%) Listeria LM _______________________________________________________ Basement   35/70  (50) 13   4 Kitchen   50/84  (60) 14   6 Front line   7/42  (17)   4   0 Sandwich line   20/56  (36) 14   3 Cases(meat, cheese 42/154  (27) 13   5 floor, seafood) Slicer (H,SL)   8/70  (11)   4   1 Slicer 1(B,deli)     5/70  (7)   2   1 Slicer 2(B,deli)     2/70  (3)   1   0 Slicer  1/103  (1)   1   0 (Cheese, basement) Slicer    0/70  (0)   0   0 (meat, basement )   _________________________________________________________________
[object Object],[object Object],[object Object],[object Object]
Back plate Table Guard front Guard back Collection  area Blade  Guard holder
Hobart Berkel Food Residue Behind the Blade
[object Object],[object Object],[object Object],[object Object],[object Object]
 
 
[object Object]
[object Object],[object Object],[object Object],[object Object]
 
[object Object],[object Object]
[object Object],[object Object],[object Object]
 
 
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
 
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
[object Object],[object Object],[object Object],[object Object]
 
 
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Transfer of Uninjured  L. monocytogenes  to Salami
 
[object Object],[object Object],[object Object],[object Object]
Risk Assessment Framework Hazard Identification Exposure Assessment Hazard Characterization [Dose-Response] Risk Characterization Description of the  Hazard (agent in the food)  And adverse effects What is the probability of consuming contaminated food AND what are the likely numbers of a pathogen in the food at the time of consumption A  mathematical model   which predicts the probability of an adverse effect from a given dose . •  Provides a RISK ESTIMATE •  What is the nature and likelihood of the health risk? •  Who and how many are likely to become ill? •  What are the sources of variability and uncertainty in the information used? Jaykus et al. 2007
Contamination Levels at  retail (cfu/ g ) Growth in the retail refrigerator Contamination level at consumption (log CFU) / g) Contamination level  per serving ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Prevalence Initial contamination level  (log cfu/g) Cross contamination  during deli slicing Growth in the home refrigerator ,[object Object],[object Object],[object Object],[object Object],Left-over portions ,[object Object],Jaykus et al. 2007
[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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Listeria monocytogenes transfer during slicing of delicatessen meats

  • 1. Dr. Elliot Ryser Department of Food Science and Human Nutrition Michigan State University East Lansing, MI September 14, 2011
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  • 7. Predicted Annual Listeriosis Cases (Log Scale) from Different Food Categories in the United States (FDA/FSIS, 2003) DM = Deli meats ; FNR = Frankfurters (not reheated); P= Pâté and Meat Spreads; UM= Unpasteurized Fluid Milk; SS= Smoked Seafood; CR = Cooked Ready-To-Eat Crustaceans; HFD = High Fat and Other Dairy Products; SUC = Soft Unripened Cheese; PM = Pasteurized Fluid Milk; FSC = Fresh Soft Cheese; FR = Frankfurters (reheated); PF = Preserved Fish; RS = Raw Seafood; F = Fruits; DFS= Dry/Semi-dry Fermented Sausages; SSC = Semi-soft Cheese; SRC = Soft Ripened Cheese; V = Vegetables; DS = Deli-type Salads; IC= Ice Cream and Frozen Dairy Products; PC = Processed Cheese; CD = Cultured Milk Products; HC = Hard Cheese.
  • 8. USDA Listeria Alternatives Implemented October 2003
  • 9. Deli Meat Recalls, 1994 - 2005 0 2 4 6 8 10 12 1994 1995 1996 1997 1999 2000 2001 2002 2003 2004 2005 2006 Year Number of recalls Ham Turkey Chicken Beef Corned Beef Other > 84 recalls > 53 million lbs
  • 10. Year Number of Recalls Recalled Product (lbs) 2006 1 375 2007 3 8600 2008 7 16,000 2009 3 39,000 2010 6 170,000 2011 6 9,500
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  • 12. Current Concerns  ~ 1,600 listeriosis cases annually including ~255 fatalities  ~ 290 of 1,600 listeriosis cases from deli meats: ~ 42 pre-sliced , ~ 248 deli-sliced  ~ 46 fatalities from deli meats  ~ 6 fatalities from pre-sliced  ~ 40 fatalities from deli-sliced
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  • 14. Distribution of Listeria spp. in a Local Deli/Restaurant ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Location Listeria L. seeligeri L. innocua L. mono ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Basement 35 20 7 8 Kitchen 50 27 11 12 Front line 7 7 0 0 Sandwich line 20 9 8 3 Seafood case 5 0 5 0 Cheese case 9 4 5 0 Meat case 7 4 3 0 Display floor 10 3 2 5 Open back case 1 1 0 0 Board (meat, cheese) 10 1 9 0 Slicer (Hobart, sandwich line) 8 7 0 1 Slicer (Bizerba, deli) 5 4 0 1 Slicer (Bizerba, cheese) 2 2 0 0 Slicer ( Hobart, basement sandwich line ) 0 0 0 0 Slicer (Hobart), basement, cheese 1 1 0 0 ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Total 170 90 50 30 ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
  • 15. _________________________________________________ Location Listeria pos/ # of positive visits # tested (%) Listeria LM _______________________________________________________ Basement 35/70 (50) 13 4 Kitchen 50/84 (60) 14 6 Front line 7/42 (17) 4 0 Sandwich line 20/56 (36) 14 3 Cases(meat, cheese 42/154 (27) 13 5 floor, seafood) Slicer (H,SL) 8/70 (11) 4 1 Slicer 1(B,deli) 5/70 (7) 2 1 Slicer 2(B,deli) 2/70 (3) 1 0 Slicer 1/103 (1) 1 0 (Cheese, basement) Slicer 0/70 (0) 0 0 (meat, basement ) _________________________________________________________________
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  • 17. Back plate Table Guard front Guard back Collection area Blade Guard holder
  • 18. Hobart Berkel Food Residue Behind the Blade
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  • 41. Transfer of Uninjured L. monocytogenes to Salami
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  • 44. Risk Assessment Framework Hazard Identification Exposure Assessment Hazard Characterization [Dose-Response] Risk Characterization Description of the Hazard (agent in the food) And adverse effects What is the probability of consuming contaminated food AND what are the likely numbers of a pathogen in the food at the time of consumption A mathematical model which predicts the probability of an adverse effect from a given dose . • Provides a RISK ESTIMATE • What is the nature and likelihood of the health risk? • Who and how many are likely to become ill? • What are the sources of variability and uncertainty in the information used? Jaykus et al. 2007
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