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CytoGene Research
&
Development
www.cytogene.in
Genetic Engineering Also known as
genetic modification or manipulation
(GM), genetic engineering is a form
of biotechnology that allows
scientists to move genes between
different species. Using various
laboratory techniques, scientists can
create life forms that could not occur
naturally. The process of genetic
engineering is intended to produce a
useful or desirable characteristic in
an organism and on a molecular level
and may include additions, deletions,
or targeted changes to the genome.
CytoGene Research &
Development ,Lucknow
provides several modules of
training as well as
dissertation/project in Genetic
Engeenring. The duration of
the programs offered for
training ranges from 7 to 30
days and for project ranges
from 3-6 months. Modules are
selected by the students on
their choice.
Duration- 30 Days
Glassware Washing & Sterilization Techniques
pH Measurement & Preparation of Buffers
Preparation of Tissue Culture Media
Callus Induction, Using Rice
Embryo/Endosperm Culture
Preparation of Agrobacterium Competent
Cells
Transformation of Agrobacterium with Binary
Vector
Agrobacterium-Mediated Transformation
Co-cultivation
Selection
Subculture
GUS assay
Transgenic Analysis by PCR
Duration- 30 Days
 Glassware Washing & Sterilization Techniques
 Isolation Bacterial Genomic DNA
 Restriction Digestion of Bacterial Genomic DNA
 Purification of Digested DNA-Column Purification
 Ligation of DNA Fragment with Cloning Vector
 Preparation of Competent Cells
 Transformation in E.coli with Recombinant Vector
 Isolation of Recombinants
 Confirmation of Insert DNA in Vector
 DNA Finger Printing
Duration- 30 Days
 Glassware Washing & Sterilization
Techniques
 Ph Measurement & Preparation of Buffers
 Isolation of Bacteria from Soil By Serial
Dilution Method
 Pouring, Streaking & Spreading of Bacteria
 Identification & Selection of E. Coli.
 Culture of E. Coli In LB Broth
 Plasmid DNA Isolation from E. Coli
 Restriction Digestion of DNA
 DNA Ligation
 Selection of Vector Genetic Transformation
of Bacteria
 Screening of The Cloned Gene From
Transformed
 Bacteria
 Analysis of Cloning
How to
Apply ?
•Choose the topic for Training/Project/Dissertation
•Download the Registration Form from our website (www.cytogene.in). Fill it
carefully.
•Attach a copy of CV (Resume)
•Attach Recommendation Letter issued by Department or Higher Authority.
•Send the document to us
By Email: Send scanned document to project.cytogene@gmail.com or
director.cytogene@gmail.com
By Whatsapp: Send the snap shot of each document to 8756658021.
By Post: Send the complete document to “The Director, CytoGene Research
& Development, 2nd Floor, B-1655, B-Block Chauraha, Indira Nagar, Lucknow
– 226016”.
By Visiting us: Submit the document by visiting us at our office.
•The Selected candidate would be informed through email.
How to Register ?
Registered Address
CytoGene Research & Development
Kishundaspur, Jaunpur, U.P. – 212401
Head Office & Laboratory
CytoGene Research & Development
2nd Floor, B-1655, Indira Nagar (B-Block
Chauraha),
Lucknow – 226016
Phone: 0522 – 2355989
Mob: +91 – 8756658021
Fax: 0522 – 2355989
cytogenernd@gmail.com
To enquire:
query.cytogene@gmail.com
To Administration:
director.cytogene@gmail.com
To HRD:
hr.cytogene@gmail.com
To R&D:
research.cytogene@gmail.com
training.cytogene@gmail.com
Project.cytogene@gmail.com
CONTACT US
FIND US ON SOCIAL MEDIA
www.facebook.com/CytoGene
https://plus.google.com/+CytogeneInlucknow
https://twitter.com/Lucknow_226016
https://www.youtube.com/channel/UCOXn4sBZGmYN9Qihvh4nOYA
www.cytogene.com
THANK
YOU

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Summer internship trainingin genetic engineering

  • 2. Genetic Engineering Also known as genetic modification or manipulation (GM), genetic engineering is a form of biotechnology that allows scientists to move genes between different species. Using various laboratory techniques, scientists can create life forms that could not occur naturally. The process of genetic engineering is intended to produce a useful or desirable characteristic in an organism and on a molecular level and may include additions, deletions, or targeted changes to the genome.
  • 3. CytoGene Research & Development ,Lucknow provides several modules of training as well as dissertation/project in Genetic Engeenring. The duration of the programs offered for training ranges from 7 to 30 days and for project ranges from 3-6 months. Modules are selected by the students on their choice.
  • 4. Duration- 30 Days Glassware Washing & Sterilization Techniques pH Measurement & Preparation of Buffers Preparation of Tissue Culture Media Callus Induction, Using Rice Embryo/Endosperm Culture Preparation of Agrobacterium Competent Cells Transformation of Agrobacterium with Binary Vector Agrobacterium-Mediated Transformation Co-cultivation Selection Subculture GUS assay Transgenic Analysis by PCR
  • 5. Duration- 30 Days  Glassware Washing & Sterilization Techniques  Isolation Bacterial Genomic DNA  Restriction Digestion of Bacterial Genomic DNA  Purification of Digested DNA-Column Purification  Ligation of DNA Fragment with Cloning Vector  Preparation of Competent Cells  Transformation in E.coli with Recombinant Vector  Isolation of Recombinants  Confirmation of Insert DNA in Vector  DNA Finger Printing
  • 6. Duration- 30 Days  Glassware Washing & Sterilization Techniques  Ph Measurement & Preparation of Buffers  Isolation of Bacteria from Soil By Serial Dilution Method  Pouring, Streaking & Spreading of Bacteria  Identification & Selection of E. Coli.  Culture of E. Coli In LB Broth  Plasmid DNA Isolation from E. Coli  Restriction Digestion of DNA  DNA Ligation  Selection of Vector Genetic Transformation of Bacteria  Screening of The Cloned Gene From Transformed  Bacteria  Analysis of Cloning
  • 8. •Choose the topic for Training/Project/Dissertation •Download the Registration Form from our website (www.cytogene.in). Fill it carefully. •Attach a copy of CV (Resume) •Attach Recommendation Letter issued by Department or Higher Authority. •Send the document to us By Email: Send scanned document to project.cytogene@gmail.com or director.cytogene@gmail.com By Whatsapp: Send the snap shot of each document to 8756658021. By Post: Send the complete document to “The Director, CytoGene Research & Development, 2nd Floor, B-1655, B-Block Chauraha, Indira Nagar, Lucknow – 226016”. By Visiting us: Submit the document by visiting us at our office. •The Selected candidate would be informed through email.
  • 10. Registered Address CytoGene Research & Development Kishundaspur, Jaunpur, U.P. – 212401 Head Office & Laboratory CytoGene Research & Development 2nd Floor, B-1655, Indira Nagar (B-Block Chauraha), Lucknow – 226016 Phone: 0522 – 2355989 Mob: +91 – 8756658021 Fax: 0522 – 2355989 cytogenernd@gmail.com To enquire: query.cytogene@gmail.com To Administration: director.cytogene@gmail.com To HRD: hr.cytogene@gmail.com To R&D: research.cytogene@gmail.com training.cytogene@gmail.com Project.cytogene@gmail.com CONTACT US
  • 11. FIND US ON SOCIAL MEDIA www.facebook.com/CytoGene https://plus.google.com/+CytogeneInlucknow https://twitter.com/Lucknow_226016 https://www.youtube.com/channel/UCOXn4sBZGmYN9Qihvh4nOYA www.cytogene.com