INCLUSIVE EDUCATION PRACTICES FOR TEACHERS AND TRAINERS.pptx
Biochemical Reaction - Prac. Microbiology
1.
2.
3. Bacteria vary in their metabolic &
enzymatic activities.
Used in identification of different
genera & species of bacteria.
Biochemical reactions are done on
bacteria grown in pure culture.
8. 1. Sugar fermentation
Sugar fermentation can be indicated
by change colour of the medium
from yellow to red.
Glucose
+ve
Lactose
+ve
maltose
+ve
mannite
+ve
Sucrose
- ve
20. Classification of a certain species of bacteria into Types.
Importance: Epidemiological investigation (e.g. food
poisoning)
Examples of Typing methods:
bacteriophage typing
bio-typing
serotyping
plasmid analysis
+ others
21. Bacteriophage Typing
Bacterial strain on surface of media + drops of different phages
homogenous bacterial growth
+ areas of lysis (= susceptibility)
23. 1- In sugar fermentation, Andrade's indicator is added
to the sugar media to test for gas production:
( ) True
( ) False
2- In the coagulase test, coagulation of plasma occurs
in positive cases:
( ) True
( ) False
3- Biochemical reactions must be done on a pure
culture of the organism to be identified:
( ) True
( ) False
24. MCQ
4-Catalase test is:
a) negative if oxygen bubbles are released
b) positive if a visible clot is formed
c) done by adding a drop of H2O2
d) done to test for the presence of urease enzyme
5- Oxidase test is:
a) done using catalase reagent
b) positive when a deep purple color is produced
c) positive after a few hours
d) negative when a yellow color is produced