14. Fig. 21-3-1
1 Cut the DNA
into overlapping
fragments short enough
for sequencing
2 Clone the fragments
in plasmid or phage
vectors.
15. Fig. 21-3-2
1 Cut the DNA
into overlapping
fragments short enough
for sequencing
2 Clone the fragments
in plasmid or phage
vectors.
3 Sequence each
fragment.
16. Fig. 21-3-3
1 Cut the DNA
into overlapping
fragments short enough
for sequencing
2 Clone the fragments
in plasmid or phage
vectors.
3 Sequence each
fragment.
4 Order the
sequences into
one overall
sequence
with computer
software.
36. Fig. 21-7
Exons (regions of genes coding for protein
or giving rise to rRNA or tRNA) (1.5%)
Repetitive
DNA that Introns and
includes regulatory
transposable sequences
elements (24%)
and related
sequences
(44%)
Unique
noncoding
L1 DNA (15%)
sequences Repetitive
(17%) DNA
unrelated to
transposable
elements
Alu elements (15%)
(10%)
Simple sequence Large-segment
DNA (3%) duplications (5–6%)
42. Fig. 21-9
New copy of
Transposon transposon
DNA of
genome Transposon
is copied
Insertion
Mobile transposon
(a) Transposon movement (“copy-and-paste” mechanism)
New copy of
Retrotransposon retrotransposon
RNA
Insertion
Reverse
transcriptase
(b) Retrotransposon movement
43. Fig. 21-9a
New copy of
Transposon transposon
DNA of
genome Transposon
is copied
Insertion
Mobile transposon
(a) Transposon movement (“copy-and-paste” mechanism)
44. Fig. 21-9b
New copy of
Retrotransposon retrotransposon
RNA
Insertion
Reverse
transcriptase
(b) Retrotransposon movement
50. Fig. 21-10
DNA
RNA transcripts Heme β-Globin
Nontranscribed Hemoglobin
spacer Transcription unit
α-Globin
α-Globin gene family β-Globin gene family
DNA
Chromosome 16 Chromosome 11
18S 5.8S 28S
rRNA ζ Ψζ Ψα Ψα α2 α1 Ψθ ε G
γ A
γ Ψβ δ β
2 1
5.8S
28S
Fetus
Embryo and adult Embryo Fetus Adult
18S
(a) Part of the ribosomal RNA gene family (b) The human α-globin and β-globin gene families
51. Fig. 21-10a
DNA
RNA transcripts
Nontranscribed
spacer Transcription unit
DNA
18S 5.8S 28S
rRNA
5.8S
28S
18S
(a) Part of the ribosomal RNA gene family
62. Fig. 21-13
Ancestral globin gene
Duplication of
ancestral gene
Mutation in
Evolutionary time
both copies α β
Transposition to
different chromosomes
α β
Further duplications
and mutations
ζ α ε γ β
ζ Ψζ Ψα Ψα α2 α1 Ψθ ε γ γ Ψβ δ β
G A
2 1
α-Globin gene family β-Globin gene family
on chromosome 16 on chromosome 11
72. Fig. 21-15
Bacteria
Most recent
common
ancestor
Eukarya
of all living
things
Archaea
4 3 2 1 0
Billions of years ago
Chimpanzee
Human
Mouse
70 60 50 40 30 20 10 0
Millions of years ago
77. Fig. 21-16
EXPERIMENT
Wild type: two normal Heterozygote: one copy Homozygote: both copies
copies of FOXP2 of FOXP2 disrupted of FOXP2 disrupted
Experiment 1: Researchers cut thin sections of brain and stained Experiment 2: Researchers sepa-
them with reagents, allowing visualization of brain anatomy in a rated each newborn pup from its
UV fluorescence microscope. mother and recorded the number
of ultrasonic whistles produced by
the pup.
RESULTS
Experiment 1 Experiment 2
Number of whistles
400
300
200
100
(No
Wild type Heterozygote Homozygote whistles)
0
Wild Hetero- Homo-
type zygote zygote
78. Fig. 21-16a
EXPERIMENT
Wild type: two normal Heterozygote: one copy Homozygote: both copies
copies of FOXP2 of FOXP2 disrupted of FOXP2 disrupted
Experiment 1: Researchers cut thin sections of brain and stained
them with reagents, allowing visualization of brain anatomy in a
UV fluorescence microscope.
RESULTS
Experiment 1
Wild type Heterozygote Homozygote
79. Fig. 21-16b
EXPERIMENT
Wild type: two normal Heterozygote: one copy Homozygote: both copies
copies of FOXP2 of FOXP2 disrupted of FOXP2 disrupted
Experiment 2: Researchers separated each newborn pup
from its mother and recorded the number of ultrasonic
whistles produced by the pup.
RESULTS
Experiment 2
Number of whistles
400
300
200
100
(No
whistles)
0
Wild Hetero- Homo-
type zygote zygote
90. Fig. 21-UN1
Bacteria Archaea Eukarya
Genome Most are 10–4,000 Mb, but
Most are 1–6 Mb
size a few are much larger
Number
of genes 1,500–7,500 5,000–40,000
Gene Lower than in prokaryotes
density (Within eukaryotes, lower
Higher than in eukaryotes
density is correlated with
larger genomes.)
Introns None in Present in Unicellular eukaryotes:
protein-coding some genes present, but prevalent only
genes in some species
Multicellular eukaryotes:
present in most genes
Other Can be large amounts;
noncoding generally more repetitive
Very little
DNA noncoding DNA in
multicellular eukaryotes