Text Version is Available at: http://www.biochemden.in/2014/11/anthrone-method-carbohydrate-determination.html Carbohydrates are very important component of Storage and structural materials in the plants. The carbohydrates are stored as free sugars and polysaccharides. The basic units of carbohydrates are Monosaccharides. When hydrolyse the carbohydrates, gives monosaccharides, but when hydrolyse monosaccharides it can not be split into more simpler sugars. The hydrolysed product of Polysaccharide are estimating by the resultant monosaccharides.

1. Estimation of Carbohydrate by
Anthrone Method
• The anthrone reaction is the basis of a rapid
and convenient method for the determination
of carbohydrates, either free or present in
polysaccharides.

2. * Principle
H2SO4
+
Furfural Anthrone
Blue Green
Complex

3. Materials
1. Anthrone reagent (0.2% in conc. H2SO4 ).
2. Glucose (10mg/100ml).
3. Colorimeter or spectrophotometer.

4. Tube # Blank St. unk.
55
50
45 15
Boiling Water Bath
Glucose (ml) 0 0.5 0.5
D. W. (ml) 1 0.5 0.5
Anthrone (ml) 4 4 4
60
30
5
10
20
40
35 25
Mix Well
COOL to Room Temperature
10 min.
Read the Optical Density at 620 nm

5. * Calculations
Absorbance of Unknown Absorbance of Standard
=
Concentration of Unknown Concentration of Standard
CUn. = AUn. ×CSt.
ASt.
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