Presentation given by Catarina Duarte from the Chemistry and Biology Technological Institute (New University of Lisbon) in the framework of the Emergence Forum Barcelona Biocat organized the Barcelona Emergence Forum (April 10-11th, 2014, Congress Palace, Montjuïc) supported by the TRANSBIO SUDOE, a translational cooperation project dedicated to innovation in life sciences in South-West Europe. The Barcelona Emergence Forum contributed to bringing together Academics, Companies, Investment Entities, Technology Platforms and Technology Transfer Offices from Spain, France and Portugal to set up collaborative projects on Human Health & Agro-food Innovation. More information at: http://www.b2match.eu/emergenceforum2014

1. Food and Health @ iBET
Catarina Duarte
Nutraceuticals and controlled Delivery Lab
IBET/ ITQB-UNL
Oeiras, Portugal

2. To create value for our partners leveraging
scientific & technological knowledge in Biology
and Chemistry
To push for faster utilization of the new
biotechnological tools in business activities;
To provide joint research advanced training –
OPEN INSTITUTION - Research conducted
through a close partnership with ITQB
To provide a support infrastructure for
academia & companies in biotechnological
processes and manufacturing in Portugal;
To support start up/spin off companies at the
incubation stage of their activities
Mission :
www.ibet.pt
Oeiras, Lisbon

3. BIAL │ IBERFAR │ JOSÉ de MELLO SAÚDE
GRUPO AZEVEDOS │ MEDINFAR │ TECNIMEDE
PHARMACEUTICALSAND HEALHCARE
AGROFOODAND FORESTRYINDUSTRIES
AMORIM CORK INVESTIGAÇÃO E SERVIÇOS │ NUTRINVESTE
RAR│ SUMOL+COMPAL
ACADEMICINSTITUTIONS
ESBUC – ESCOLA SUPERIOR DE BIOTECNOLOGIA (UCP)
FCT-UNL – FACULDADE DE CIÊNCIAS E TECNOLOGIA (UNL)
ITQB – INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA (UNL)
FACULDADE DE CIÊNCIAS MÉDICAS (UNL)
REITORIA DA UNIVERSIDADE NOVA DE LISBOA
FCT – FUNDAÇÃO PARA A CIÊNCIA E TECNOLOGIA
FLAD – FUNDAÇÃO LUSO-AMERICANA PARA O DESENVOLVIMENTO
IAPMEI – INSTITUTO DE APOIO ÀS PEQUENAS E MÉDIAS EMPRESAS
IICT – INSTITUTO DE INVESTIGAÇÃO CIENTÍFICA TROPICAL
INIAV – INST. NACIONAL DE INVESTIG. AGRÁRIA E VETERINÁRIA
FOUNDATIONSAND INSTITUTES
In total, iBET is 60% privately owned
Non-for-profit
Member of the LAO (ITQB, IGC, iBET, CEDOC)

4. 16
fully equipped
laboratories
Pilot Plant
cGMP Analytical
Services Unit
cGMP CMO (GeniBET)
Human resources:
18 PhDs
4 Engineers
67 Researchers
14 Lab Technicians

5. FLEXIBILITY & CONFIDENTIALITY
SPECIFIC NEEDS
• Contract Research Projects
• “Umbrella Research Contracts”
(plus per project based contracts)
• Satellite Laboratories
(Merck Serono and Bayer Healthcare)
• Joint Research for Advanced Training
(MSc, PhD, publications submitted after both parties approval)

6. iBET is a certified Service Provider
Agro-forestry
Biopharmaceuticals and novel therapies
cGMP Analytical services and Bioassays
Food in Health
Food, Water and Energy Nexus
Protein stability, structure and drug design

7. BioactivityChemical Characterization
and Metabolism
Process Tecnology
Analytical characterization:
Phytochemicals
Carbohydrates
Proteins
Sensory analysis
Metabolism:
• in vitro and in vivo digestion
and bioavailability
• metabolomic
Extraction and Fractionation
(green and sustainable processes)
Fermentation
Formulation
(development of bioactive and
bioavailable forms)
Scale-up
(extraction, fermentation, drying)
Toxicity assessment
Health benefits
(cell models, yeast models, …)
Preservative effects
Molecurar Structure - Bioactivy
Food and Health

8. Chemical Characterization and Metabolism
Analytical characterization:
Phytochemicals
Carbohydrates
Proteins
Sensory analysis
Metabolism:
• in vitro and in vivo digestion
and bioavailability
• metabolomic
Molecurar Structure - Bioactivy
Food and Health
Spectrophotometric
assays
Chromatographic
techniques
• Total polyphenols content
• Total flavonoids content
• Total phenolic acids content
• Total procyanidin content
• Total anthocyanin content
• Total betalain content
• Analysis of carotenoids
• TLC
• UPLC
•HPLC-DAD-FD-ED
• GC-MS
• FTIR
• NMR

9. Food and Health
Process Tecnology
• Conventional Extraction (with
biocompatible solvents)
• Pressurized Liquid Extraction
• Supercritical Fluid Technology
• Adsorption processes (molecular
imprinted polymers)
Bioactive rich fractions
(e.g. polyphenols,
carotenoids, phytosterols,
terpenes,...)
Natural colours
Natural flavours
Natural preservatives
Application in food, cosmetic or pharma industries
Extraction /Fraccionation of Natural Compounds

10. Food and Health
Process Tecnology
Materials Processing – particle formation, encapsulation and impregnation
Fundamental
studies
Design & optimization
of Delivery Systems
DDS Characterization
(morphology, particle size,
pharmacokinetics,…)
Nanotoxicity
Supercritical fluid
Technology
Solid material processing with
ScCO2
Particle formation technologies
Impregnation by adsorption
Molecular Imprinting
Spray drying
Spray chilling
Fluidized-bed spray
“Conventional”
Techniques

11. Food and Health
Bioactivity
Characterization and pre-clinical Bioactivity evaluation of bioproducts
Enzymatic
assays
Cell-based
assays
Chemical
assays
 In vitro chemical and cell based assays
for pre-clinical studies (antioxidant,
anti-inflammatory, anti-proliferative,,
neuroprotection, cardioprotective,
anti-diabetes…)
 Transport and permeability studies (in
vitro)
 Bioavailability evaluation after
digestion (in vitro)
Molecurar Structure - Bioactivy

12. Catarina Duarte
Contacts:
cduarte@ibet.pt
http://www.ibet.pt/services/food-health