Presentation given by Sonia Da Rochas from Novaptech, in the framework of the Emergence Forum Barcelona Biocat organized the Barcelona Emergence Forum (April 10-11th, 2014, Congress Palace, Montjuïc) supported by the TRANSBIO SUDOE, a translational cooperation project dedicated to innovation in life sciences in South-West Europe. The Barcelona Emergence Forum contributed to bringing together Academics, Companies, Investment Entities, Technology Platforms and Technology Transfer Offices from Spain, France and Portugal to set up collaborative projects on Human Health & Agro-food Innovation. More information at: http://www.b2match.eu/emergenceforum2014

1. Biotechnological development of Aptamers
Dr. Sonia Da Rocha Gomes
Emilie Daguerre
Laetitia Evadé-Farrugia
Novaptech,
146 rue Léo Saignat, bat 3A, BP12
Bordeaux, France
sonia.darocha@novaptech.com
+33.5.57.57.95.05
10th April 2014, Human Health Track,
Barcelona

2. Novaptech, business unit
-Created in 2008,
-3 people, Bordeaux
-Dedicated to the biotechnological
development of APTAMERS
-Managed by the association
-Connected to the academic lab Inserm U869,
Dr. Toulmé ’s team > 15 years of expertise in
aptamer field (11th, Agrofood session)
- Two automated platforms of selection and
aptamer production, unique in France
Aptamer production
- Identification
- Optimization
- Conjugation
Aptamer-based tools
in analytical,
diagnostic
or therapeutic fields
Method
development
-Implementation
of new strategies
-Improvement of the
automated platforms
OFFER :
Aptamer selection
against your target and
characterization
Service contracts with
academic labs and
companieS
 30 projects

3. Your needs,
looking for new molecular probe
DETECTION AND MONITORING
(molecular imaging,
biosensors, sandwich assay)
PURIFICATION
(affinity capture, sorting)
CHARACTERIZATION
(target validation,
protein-binding RNA sites)
INHIBITION
(drug delivery, drug discovery)REGULATION
(artificial riboswitch)
Aptamers, the right solution

4. Aptamer properties,
Structured oligonucleotides identified:
 from randomly synthesized library
 against a target
 through combinatorial strategy (SELEX)
iterative rounds of selection / amplification
(Tuerk and Gold,1990; Ellington and Szostak, 1990)
 High affinity and specificity
 Rival antibodies
 Chemical synthesis
 Chemically modified and
conjugated to numerous pending
groups (fluorophore,
radioelements, biotin..)

5. Aptamer selection process,
146 rue Léo Saignat, bat 3A, BP12
Bordeaux, France
sonia.darocha@novaptech.com
+33.5.57.57.95.05
Phase 1
Phase 2
Automated platform for aptamer selection,
unique in France (Inserm U869 lab)
Aptamer
Selection
Screening
(Affinity)
Cloning
Sequencing,
Sequence analysis
Candidates
production and
binding validation
Optimization
- Minimizing their size
- Improving nuclease resistance
- Conjugation: biotine, amine,
fluorochrome..