1. Will you know next week – or in fifteen years? EverTrieve ™ Catalogued, tamper-proof storage of large data files Do you know where your flow data are? Do you know what was in your experiment? with Cytogenie ™ in a self-managing on-line/off-line archive

2. Where do I start? Information flow in a typical biomedical study Publications, Patents Study Questions, Hypothesis Experiments Answers, Interpretations Instrument info Annotated Raw Data Sample info Annotated Sample Data Subject info Annotated Subject Data Statistical Analysis Experiment info Annotated Tube Data Tube an d reagent info Measurement instrument e.g., FACS Reagent sets A B A B Samples from#1, 2 Tubes U U U U Subjects #1, 2…

3. 1. Start at the beginning Plan your experiment with CytoGenie FacsXpert CytoGenie

7. FACS Curator 2. Keep EverTrieve’s FACS Curator on your instrument computer(s) FacsXpert Your instrument computer

8. Keywords Labels FACS Curator labels and keywords Instrument settings DiVa FACS Curator to DiVa* passes instrument settings Instrument settings Labels Keywords * Integration with other instrument software on demand FacsXpert Your instrument computer FacsXpert CytoGenie Your experiment protocol

9. FACS Curator bundles collected data with your protocol in a tamper-proof wrapper collected data + protocol collected data protocol Tamper-proof wrapper

11. FACS Curator passes the tamper-proof wrapper to the EverTrieve server tamper-proof wrapper EverTrieve FacsXpert Your instrument computer FACS Curator

12. EverTrieve catalogs the content of each bundle of files, including experiment descriptions, instrument settings, reagents, keywords, subjects, samples, and other metadata from CytoGenie EverTrieve

13. EverTrieve emails the catalog with a link to the data to the researcher and anyone he or she designates email EverTrieve

14. Researchers can search the EverTrieve catalog in the email or on a password-protected website

15. Will you know next week – or in fifteen years? EverTrieve ™ Do you know where your flow data are? Do you know what was in your experiment? with Cytogenie ™ EverTrieve has been running for over three years in the Stanford Shared FACS Facility, serving over 400 users and collecting 10GB of flow data from eight different instruments every week. It has not lost a single data bit.