2. Background of Membrane separation
technology
• Simple filtration technology which can separate
molecules according to their molecular size
• No heat treatment
→ Low Initial Cost, Small Energy,
High Quality of Products
3. Pore size
Permeate flux of water at 38 bar,
Name of membranes Molecular weight
24° (LMH)
Å Ångström nanometer
UM 05 500 21 2.1 17
UM 2 1 000 24 2.4 34
UM 10 10 000 30 3.0 102
PM 10 10 000 38 3.8 935
PM 30 30 000 47 4.7 850
XM 50 50 000 66 6.6 425
XM 100A 100 000 110 11.0 1105
XM 300 300 000 480 48.0 2215
4. Objective of the Work
Development of a membrane separation
process which can efficiently purify anserine-
carnosine (AC) extracted from chicken meat
5. Materials
(preparation of chicken extract)
water:carcasses=3:1 Chicken carcass
Water
Extraction 100˚C, 4-6 h
Ion exchange resin
Removal AC: 6.25 g/L (235)
Acidic and neutral Creatinine: 2.31 g/L (113)
amino acids Na+: 0.27 g/L (56)
NF membrane
6. Apparatus
Lab-Module type 20.(DSS)
Maximum membrane area:
7200 cm2 (180 cm2×40 sheets)
Maximum pressure: 6 MPa
Maximum temperature: 100˚C
pH-range: 0-14
8. Total Circulation Experiment
P
F
I
I
P
Retentate
I
Feed
Pump
Permeate
Feed flow rate: 5.8 - 11.3 L/min
Feed tank
Pressure: 1 - 6 MPa
Temperature: 25 ˚C
9. Batch Wise Experiment
P
F I
I
P
Retentate
I Creatinine and
Permeate Sodium Ion
Feed
Area of membrane: 360 cm2
Pump
Temperature: 25 ˚C
Initial weight of Feed: 11.2 kg
Feed tank Final weight of Feed: 3 kg
10. Analyses and Calculation of Rejection Value
HPLC using column (TSKG2, 500PWXl) with
45% acetonitrile (pH3.0).
ICP-AES (JICP-PS3000UV; Leeman Lab).
b
.. R
obs = 1−
Cp
Cr
Robs: Observed rejection
Cp: Concentration of permeate
Cr: Concentration of retentate
11. Results of Total circulation experiment
Effect of operating pressure ( P) on permeate flux (Jv) and
observed rejection for anserine-carnosine (RAC), creatinine (RCr)
and sodium ion (RNa) with NFT50 membrane
12. Results of Total circulation experiment
Effect of flow rate on permeate flux (Jv) and observed
rejection for anserine-carnosine (RAC), creatinine (RCr) and
sodium ion (RNa) with NFT50 membrane
14. Results (Batch-Wise Concentration)
Changes in purity, yield, concentration of anserine-carnosine and
permeate flux value (Jv) during Batch wise experiment with NFT50
membrane (flow rate:10 L/min, pressure:4 MPa)
15. Proposal of a Mathematical Model
A: membrane area
dV Jv: Permeate flux
= − A Jvρ (1)
dt V: volume of solution
d (C AC V f ) t: time
= − A J v Cac (1 − RAC ) (2)
dt C: concentration of components
R: rejection of components
J v = D − k ln(CF ) (3)
CF: concentration factor
V0 D: constant
CF = (4)
Vf k: mass transfer coefficient
16. Simulation Results
Changes in purity, yield, concentration (CAC) of anserine-carnosine
and permeate flux value (Jv) with NFT50 membrane (experimental
value and calculation line)
17. Process design
Chicken carcass 5 t/d
Water 15 t
Extraction 4-6 h
Extract 15,000 kg
Ion exchange resin
Removal
Acidic and neutral Eluted solution 1,400 kg
amino acids NF membrane NFT50 membrane
Permeate 1.2 m2, 6 h
Retentate: 150 kg
Creatinine and sodium ion
Anserine-Carnosine
(conc.: 7.5%, purity: 90%, yield: 98%, AC: 11.2 kg)
18. Whey
Purification
/centryfuge and cake filter/
Retentate
Permeate
Curd and
UF membrane 3 kind of UF
Retentate membrane
Permeate
Solvent protein’s
NF membrane 3 kind of NF
Permeate
membrane
Retentate
Solvent
Lactulose
(purity: 90%, yield: 95%)
