Detail Mass production of.... Trichoderma viride Corcyra cephalonica cryptolaemus montrouzieri Trichogramma chilonis Zygogramma bicolarata Nuclear polyhydrosis virus of Helicoverpa armigera Nuclear polyhydrosis virus of Spodoptera litura. in this ppt you will get all detail mass production procedure of all mentioned above bio pesticides and bio agents.

1. MASS PRODUCTION OF
BIOAGENTS
&
BIOPESTICIDES
Balram Solunke
balram2424@gmail.co

2.  CONTENT:
 Mass production of....
 Trichoderma viride
 Corcyra cephalonica
 cryptolaemus montrouzieri
 Trichogramma chilonis
 Zygogramma bicolarata
 Nuclear polyhydrosis virus of Helicoverpa
armigera
 Nuclear polyhydrosis virus of Spodoptera
litura

3. Mass production of
Trichoderma viride
Materials :
1)Potato
2)Dextrose
3)Glass wares
4)Spirit lamp
5)Pure culture of fungus
6)Laminar air flow
7)Ethyl alcohol
8)Cotton plug, rubber band,alluminium foil
etc.

4. Inoculation:

5. MIXING OF CULTURE

6. mix well with talcum powder for final product of
Trichoderma viride

7. mix well with talcum powder for final product of
Trichoderma viride

8. Packing:

9. Prepare POB broth media
Cooking of 200gm potato for 1 lit media than
extract taken & add water &mix 20gm dextrose
Than make final volume 1 lit
Procedure

10. Fill media in 1 lit conical flask & plugged
with aluminum foil than tied with rubber
band
Sterilize media & requiered glasswares like
test tube petriplates & inoculation bottles
for 15 min in 1210c in autoclave
Kept these for cooling

11. Sterile LAF with ethyl alcohol than transfer
glass wares & media in LAF
Pour media in bottle , Petridis
Inoculate pure culture of trchoderma
viridae with the help of inoculating needle
using spirit lamp to avoid contamination

12. Collect the fungus growth & crush these
culture with the help of blander mix these
with the help of blander
After that mix well with talcum powder for
final product of Trichoderma viridae
Drying of product at room temp for 1 day

13. Mix CMC (carbon methyl cellulose) as
sticky agent in dried powdery form
Packing product in polythene bags &
labeling

14. Mass production of
Corcyra cephalonica
Materials :
1) One rearing room of 15x12x8 cm size with
a.c installation
2) Wooden tray of 45x30x15 cm size
3) Coarsely ground grains of sorghum/bajra
4) Yeast
5) Eggs of corcera

15. Procedure sorghum / bajra
Grains should be milled coarsely to make 3-4
pieces of each grains
Heat/sterilized in an oven at 1000 c for 30 min

16. Spray 0.1% formalin solution uniformly & mix well
Dry the grain with fan air
Pour 5 kg coarsely ground grains of jawar or bajra
in each wooden box & add 10gm yeast powder +
200gm g.nut

17. Obtain pure culture of corcyra cephalonica
Mix 1cc 18000 to 20000 egg in each box
Cover box for 30 days approximately

18. Maintain temperature of rearing room at 300c
After 20-40 days on alternate day collect adults
Put 1000 adults oviposition cage

19. Collect the deposited eggs, clean from scales &
debris
Store for 3-4 days in refrigerator at 100c it
requiered,these eggs treat with UV rays for 45 min.

24. Mass production of
cryptolaemus montrouzieri
a predator on mealy bug

25. REARING OF MEALY BUG ON
POTATO :

27. Fill wooden/plastic trays with sandy silt soil up to
2 to 3 cm deapth for planting
Place 10-15 potato tubers & cover with moist soil
Fill such tray frequently
1) REARING OF MEALY
BUG ON POTATO :

28. Watering such trays
Put ovisacks of mealy bugs over potato sprouts
With in 20-25 days mass culture of mealy bugs
could be obtained such trays
Maintain the temperature in rearing room
between 20 to 300c

29. REARING OF MEALY BUG ON
RED PUMPKINS :

30. 1) REARING OF MEALY
BUG ON RED PUMPKINS :
Medium sized with ridges & furrows & grooves
with small stack & pumpkin should be selected
for the mealy bug rearing
Clean with water to remove dust particles

31. The wound if any pumpkin plug with paraffin wax
Than tied the whole pumpkine with thread for
griftting the mealy bug on it
Treat 0.1% bavistine solution (1gm/lit) to prevent
it’s rotting during rearing process

32. Collected mealy bug release on pumpkin
Cages placed on working tables/racks in tearing
room
Keep the pumpkin in wooden cage

33. 1) REARING OF
PREDATOR
cryptolaemus montrouzieri :
When mealy bugs are 8-10 days old a stock of
15-20females of cryptolaemus montrouzieri at
temperature of 20-300c
The released female feed on mealy bugs eggs ,
numph & adults

34. On hatching larvae feed on mealy bug
Female deposit eggs on pumpkins
Put fully developed larvae with mealy bugs on as
food plastic jar & provide paper strips for its
pupation
The emerging beetles collect in plastic bowl &
feed with honey agar or mealy bugs

35. REARING OF PREDATOR
cryptolaemus montrouzieri

37. MASS PRODUCTION
TECHNIQUES OF
TRICHOGRAMMA CHILONIS

38. Procure eggs of corcyra cephalonica
Paste 1cc (18000 to 20000) egg on thinly
smeared gum arabic on egg card with the help of
suitable strain
Expose the eggs to UV rays for 45 min
On backside of the trichocards written down the
mentioned information

39. Transfer 6 days old daily parasitoid egg card with
1:6 ratio (parasite : host) for 24 hrs.
After 4 days of parasitization brush out host
larvae of day hatched
Such parasitized eggs cards could be stored for
about 20 to 30 days at 100c temperature in
refrigerator

40. PREPARATION OF TRICHOCARD
:

43. MASS PRODUCTION
TECHNIQUES OF
Zygogramma bicolarata

45. Take parthanium leaves in transparent plastic
container
Release 10 pairs of beetles female& male on
such leaves
Egg laying observed in leaves replaced & fresh
bouquets are provided

46. these process repeated for month
After few days eggs collect in plastic small bottle &
kept leaves of parthanium in bottle
Emergence of larvae & feed on leaves & pupa in
soil

47. 15-20 small plants transplanted in aluminum cage
these cages yield about 100-125 adults

49. MASS PRODUCTION
TECHNIQUES OF NUCLEAR
POLYHYDROSIS VIRUS OF
HELICOVERPA ARMIGERA

51. FIELD COLLECTION OF
HELICOVERPA ARMIGERA FROM TUR
FIELD :

52. PREPARATION OF ARTIFICIAL
DIET :

55. MATING OF HELICOVERPA
ARMIGERA MOTH IN GLASS
CHMBER :

56. PUPATION BOX :

60. Select late third instar or early 4th instar larvae of
HELICOVERPA ARMIGERA for virus inoculation
Pre- strave these larvae for 6-8 hrs. before virus
inoculation
Introduce piece of the virus contaminated diet in
each container with pre-starved larvae

61. The larvae begin to show symptoms of virus
infections after about 4-5 days
Collect the dead diseased larvae in distilled water
Replace uneaten part of diet after 2-3 days &
clean the containers

62. Pullet staved at the bottom may be discard &
centrifuge at 2500 rpm for 10 min
Supernatant is discard & pellet mix in water 10
times in purified virus
Filter this suspension through double layers of
muslin cloth & centrifuge for 3 to 5 min at 500 rpm

63. MASS PRODUCTION
TECHNIQUES OF NUCLEAR
POLYHYDROSIS VIRUS OF
SPODOPTERA LITURA

65. Collection of SPODOPTERA LITURA from cabbage field :

66. Prepare NPV suspension in water for leaf
treatment
Pluck castor leaves with the petiole & dip in the
virus solution
Dry treated leaf under shade
Starve larvae for 4 to 5 hrs before feeding

67. Keep the news paper at the bottom of bucket &
provide the trated leaf for feeding cover mouth of
bucket with cloth
Everyday remove the excreta of larvae
Provide untrated leaf for feeding

68. Larvae will start dying after 4-5 days
Collect the dead larvae in distilled water & grind
the larvae thoroughly stir the solution & filter it by
cloth
Collect filtrate store in amber coloured bottles in
colol place

69. Thank You