Lynntech's Microfluidics Presentation at COMSOL Boston Conference 2009. Anjan Contractor.

1. s c i e n c e | e v o l v e d Multiphysics Simulation of Isoelectric Point Separation of Proteins Using Non-Gel Microfluidics System Anjan Contractor, Ashwin Balasubramanian, Gareth Hughes

2. Mission To nurture and harvest scientific creativity to produce life changing technologies

4. Current Protein Diagnostic Techniques Expensive Immobile Hard to Maintain Slow Protein Analysis -Enzyme-linked Immunosorbent Assays (ELISAs) -2D Gel Electrophoresis Protein Detection -Mass Spectrometer

5. Remote Area Diagnostic alqi.org http://oregonstate.edu/dept/ncs http://www.defenseindustrydaily.com Yon M. Achieves. http://www.michaelyon-online.com

7. Lynntech’s Concept: Isoelectric Point based Protein Separation Chip Top Inlet : pH, Ionic Strength, flow rate Middle Inlet: pH, Ionic Strength, flow rate Bottom Inlet: pH, Ionic Strength, flow rate L W V, Electrode Objectives: -High pH gradient at the exit of microfluidic channel. -Multiple chip configuration that can achieve pH resolution of 0.1

10. COMSOL Model Set-up Incompressible Navier-Stokes Density=998 kg/m3 Dynamic Viscosity=1.002e-3 Pa-s Inflow Velocity Pressure 0 Pa Subdomain Setting Boundary Setting No Slip at the Wall

11. COMSOL Model Set-up PDMS Fluid Average Relative Permittivity Copper Electrode +V -V Electrostatics Subdomain Setting Boundary Setting Continuity

12. COMSOL Model Set-up Electrokinetics Subdomain Setting Boundary Setting

13. COMSOL Results pH Distribution at the Exit of the Channel No External Potential

14. Statistical Optimization from COMSOL Results: Max pH p i c i f 1 = Σ (i=0,10) (c i *p i ) where f 1 =Maximum value of Δ pH, c i =constant, p i =parameter p i

15. Statistical Optimization from COMSOL Results: Uniform pH Maximum pH Uniformity at the Channel Exit p i c i f 2 = Σ (i=0,10)(c i *p i ) where f 2 =Maximum pH Uniformity, c i =constant, p i =parameter

16. COMSOL Results based on Statistical Analysis No External Potential

17. COMSOL Results based on Statistical Analysis No External Potential

20. s c i e n c e | e v o l v e d 1313 Research Parkway College Station, TX 77845 P. 979.693.0017 F. 979.694.8536 www.lynntech.com