IMT Assays

1. Industrial Microbial Testing (IMT)
Andy Moreno
Vice President, Microbiological Sales Engineering
Industrial Microbial Testing (IMT)
Industrial Microbial Testing (IMT)
Cell: +559‐827‐8245
FAX: +866‐776‐3746
andy.moreno@imt‐pcr.com
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http://www.imt‐pcr.com
Linkedin Profile
Skype: Andy Moreno
TBC Facebook
May 19, 2010
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2. IMT qRT‐PCR Assays
• Total Bioburden Assays
– Total Bacteria Count Assay
– Total Yeasts & Molds Count Assay
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• Commercialized Pathogen Assays
– E. coli spp. Assay • Primers
– Salmonella spp. Assay
• Controls
– C. botulinum “E” A
C b t li “E” Assay
– Porcine Assay • Molecular Water
• R&D Pipeline
– Campylobacter Assay
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– Listeria spp. Assay
– Enterococcus spp. Assay
– Staphylococcus spp. Assay
– Pseudomonas spp. Assay
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– Xanthomonas spp. Assay
– Mycoplasma spp. Assay
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3. IMT’s Total Bacteria Count (TBC)*
an “Internal Investigation Tool”
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• Sample size: as desired by client
• Limit of detection: One (1) cell in 5 µl sample
– Determined by sample preparation
• Time to result‐‐collection to result: ~One (1) hour
• Sample run time:
Sample run time:
– Thermocycler #1: ~thirty (30) minutes
– Thermocycler #2: ~ninety (90) minutes
• I l i it All b t i ( k
Inclusivity: All bacteria (prokaryotes)
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• Exclusivity: Eukaryotic DNA (non‐bacterial)
*Patent Pending Slide 3 of 11

4. IMT’s Yeasts and Molds Assay*
an “Internal Investigation Tool”
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• Sample size: As desired by client
• Limit of detection: One (1) cell in 5 µl sample
– Determined by sample preparation
• Time to result‐‐collection to result: ~One (1) hour
• Sample run time:
Sample run time:
– Thermocycler #1: ~thirty (30) minutes
– Thermocycler #2: ~ninety (90) minutes
• Inclusivity: All yeasts & molds (eukaryotes)
Inclusivity: All yeasts & molds (eukaryotes)
• Exclusivity: Prokaryotes
*Patent Pending Slide 4 of 11

5. RT‐PCR Systems which can employ IMT products
Rotor‐Gene Q Roche LightCycler 2; LightCycler 480 ABI 7500 DxNA
GeneSTAT
Corbett Research
Corbett Research Eppendorf Cepheid
Rotor‐Gene 6000 SmartCycler
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6. IMT Product Lines by Industry
Pharma
Food Aerospace
QA/QC
Total Bioburden Assay
Total Bacteria Count Total Bacteria Count (TBA)
Yeasts & Molds Count NASA‐Boeing
Total Bacteria Count
Total Yeasts and Molds
Listeria
Salmonella
E. Coli
E C li
C. Bot. “e”
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7. Total Bioburden Assays: Pharma QA/QC
• Total Bacteria Count (TBC) and Total Yeasts and
Molds Count (TYMC) offer rapid results for Total
Molds Count (TYMC) offer rapid results for Total
Bioburden Assay.
• Benefit: Speed as compared to four (4) day culture
Benefit: Speed as compared to four (4) day culture
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8. Food: Positive Release Program
• Irrigation water tested
• Raw fruit and vegetables are washed and cleaned
• Peracetic Acid and Chlorine wash bath tested at the end of each run
• Fi i h d
Finished product is tested
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• Environmental samples collected in plant “hot spots” Slide 8 of 11

9. IMT’s Tests at an Aseptic Packer
• IMT’s Assay Implementation for DNA‐tested ‐HACCP
– Reverse Osmosis Water Testing
– Product in‐process testing
– Final Product Testing
– Yeasts and Molds Testing
easts a d o ds est g
– Shipment “Release” upon Final Product Testing report
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10. Current Industrial Clients
 Major Biopharma Company: USA
 Fresh Vegetable Packer: California, USA
 Aseptic Beverage Packer: California, USA
 Aerospace Agency QA/QC: California USA
 Biotechnology Equipment Manufacturer: USA
 Agricultural Agency—Water Testing: Mexico
 Food Preparation Factory: California, USA
 USDA, Ames, Iowa, USA
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Biotechnology Production Co., New Jersey, USA
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11. Questions & Answers
Questions & Answers
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