Novel saltwater AOC assay optimizes desalination pretreatment
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Presented at the May 24, 2010 14th Annual Water Reuse & Desalination Research Conference in Tampa Bay, FL. The title of the talk was "A Novel Saltwater AOC Assay for Optimizing Desalination R/O Pretreatment" and described a new way of measuring small organic carbon molecules in seawater that serve as food for bacterial growth. Growth of the bacteria on reverse osmosis membranes used to treat the seawater causes clogging problems and increases the energy required (and greenhouse gas emissions) and maintenance (more frequent cleaning).
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Novel saltwater AOC assay optimizes desalination pretreatment
- 1. A novel saltwater AOC assay for optimizing desalination R/O-pretreatment Lauren Weinrich, Orren Schneider, Eugenio Giraldo, and Mark LeChevallier
- 12. AOC Application and Results
- 14. AOC: parallel analysis Plate counts Luminescence - Limited to single time point per day - Programmable - Culture period - Measured at hourly intervals - Labor - Better estimate of maximum http://www.sigmaaldrich.com/analytical-chromatography/microbiology/learning-center/theory/introduction.html
- 18. Tampa Bay desalination process schematic Precoat/Diatomaceous earth filters Junction box Seawater pump station Coagulation basins Sand filters Chlorine dioxide Sulfuric acid Ferric Cartridge filters To RO Bisulfite Post- Pre- Post- Raw ClO 2 sand sand Post- Post- . Cart. DE
- 22. Field testing – Tampa rates of AOC utilization
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- Constitutive luminescence rather then the high cell-density dependent bioluminescence
- Constitutive luminescence rather then the high cell-density dependent bioluminescence
- Cost ~$200 through ATCC
- Measuring AOC was made convenient though the use of sensitive, automated, photon-counting luminometer. We can take fast measurements of the bacteria’s growth & luminescence response in convenient 96-well microtiter plates, which greatly reduces labor associated with the original test. The traditional test monitored growth by plate counts and serial dilutions and proved to be too cumbersome for widespread application, particularly in utilities.. NEXT SLIDE
- Lauria broth agar plates, 24 hours of growth at 30 deg C
- Understand treatment train, location of sampling points and the impact of each individual treatment step
- Testing desalination samples collected over 3 consecutive days at Tampa Bay for assimilable organic carbon (AOC). AOC levels were greatest before sand filtration indicating variability of the source water quality and most of the AOC removal occurred after the sand and diatomaceous earth filtration. Removal of this biodegradable fraction of carbon is important for protecting RO membranes from biofouling. TOC ranged from 4.9 – 5.4 ppm
- TOC range 4.2 – 5.3 mg/L
- Algal bloom, Alafia river dredging