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Polymerase Chain Reaction

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simple description of PCR including questions and answers at the end.

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Polymerase Chain Reaction ,[object Object],[object Object],[object Object]
Uses of PCR ,[object Object],[object Object],[object Object],[object Object],[object Object]
DNA Selection ,[object Object],[object Object],[object Object]
The Reaction Mixture ,[object Object],[object Object],[object Object],[object Object],[object Object],A T G C A T C T A C G
PCR Machine ,[object Object],[object Object]
Splitting DNA ,[object Object]
Adding Primers ,[object Object],[object Object],[object Object],G C A U A 5’ 5’ G C A U A 5’ T A G G C A T A G C C T T A T C C G T A T T C G T A T C C G T A T C G G A A T A G G C A T A A G C A 5’
Adding Nucleotides ,[object Object],[object Object],[object Object],A T C C G T A T C G G A A T A G 5’ T A G G C A T A G C C T T A T C C G T A T T C G T G C A U A 5’ G C C T T A T C C G T A T T C G A 5’ G C A U A A T C C G T A T C G G A A T A G G C A T A A G C A 5’
Repeating the cycle ,[object Object],[object Object],[object Object],A T C C G T A T C G G A A T A G G C A U A 5’ C G T A T C G G A A T A G G C C T T A T C C G T A T T C G A 5’ G C A U A G C A U A 5’ 5’ G C A U A G C C T T A T C C G T A T
1 3 2
Continuing the Cycle ,[object Object],[object Object],10 9 8 7 6 5 4 3 2 1 0 Cycle No 2026 1004 494 240 114 52 22 8 2 0 0 Short Fragments 22 20 18 16 14 12 10 8 6 4 2 Long fragments
30 Cycles ,[object Object],[object Object]
Summary Heat to 95 0 C Denature DNA Cool to 60 0 C Anneal Primers PCR Cycle Add Nucleotides Heat to 72 0 C Heat to 95 0 C Denature DNA Cool to 60 0 C Anneal Primers Heat to 72 0 C Add Nucleotides
Questions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Questions Continued ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Questions continued ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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Polymerase Chain Reaction

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  • 10. 1 3 2
  • 11.
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  • 13. Summary Heat to 95 0 C Denature DNA Cool to 60 0 C Anneal Primers PCR Cycle Add Nucleotides Heat to 72 0 C Heat to 95 0 C Denature DNA Cool to 60 0 C Anneal Primers Heat to 72 0 C Add Nucleotides
  • 14.
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  • 16.