1. Unhwa Corporation
Ddobyul Plant Stem Cell Introduction
This document is CONFIDENTIAL and the property of Unhwa Corporation. It is intended solely for
the use of the individual to whom it is addressed or any other recipient expressly authorized by
Unhwa Corporation.
Any unauthorized disclosure, reproduction, copying, distribution or use of this document is strictly
prohibited.
Copyright by 2005-2012 Unhwa Corporation. All rights reserved.
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2. Power of Plant
Power of Plant
2

3. Power of Plant
The oldest
living organism
on earth?
Bristlecone Pines in
California, U.S.,
which have been
alive for 4,772 years.
3

4. Power of Plant
The largest
living organism
on earth?
the Giant Sequoia in
California, U.S., with 87
meters in height and
2,000 tons in weight
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5. Power of Plant
Ginkgo tree is also known as
“living fossil”,
as it coexisted with dinosaurs
250 million years ago and
survived through Ice Age with
strong vitality.
Adonis annua is a
perennial plant which does
not lose its vitality, even
blooming flowers breaking
through the snow
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6. Power of Plant
Endless Living
Cycle for Plants
One-way aging
process for animals
and human beings
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7. Secret of Plant’s Vitality
Secret of Plant’s Vitality
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8. Secret of Plant’s Vitality
Plant stem cell is an immortal cell and the origin of plant vitality in which
the growth and regeneration of plant is programmed in its entirety.
VASCULAR
CAMBIUM
VASCULAR
CAMBIUM
“Plant stem cells, contained in specialized structures called
meristems, have amazing regenerative powers.
They enable plants to grow and produce new organs
throughout lifetimes that can span hundreds of years.”
- ’Nature’ 415, 751-754 (2002)
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9. Secret of Plant’s Vitality
- Massive Multiply: exact duplication
- Generate other Plant Organs
“They enable plants to grow and produce new organs
throughout lifetimes that can span hundreds of years.”
- ’Nature’ 415, 751-754 (2002)
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10. Secret of Plant’s Vitality
-Regeneration Power
reason of why trees can grow again in the spring
Prepare for a new
Preserve lots of lifecycle
necessary materials for
Plant Stem Cell regeneration
has Great
Regeneration
Power
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11. Secret of Plant’s Vitality
- Source of Plant’s Surviving Strategy
Plant stem cells make useful compounds for the whole plant to
survive in the harsh environment.
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12. Secret of Plant’s Vitality
However due to the distinct structural characteristics of plant vascular stem cell,
none has succeeded in isolation and culture of plant vascular stem cell (vascular
cambium).
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13. Ddobyul® Plant Stem Cell
Ddobyul® Plant Stem Cell
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14. Ddobyul® Plant Stem Cell
In 2005, Unhwa Corporation succeeded in
the world’s first isolation and culture of plant stem cell.
This pure plant stem cell is trademarked as Ddobyul®.
Following the success of isolating plant stem cell, in 2006,
Unhwa Corporation began joint research with University of
Edinburgh Institute of Molecular Plant Sciences, a world-
renowned biotechnology research institute.
The research outcome of plant stem cell isolation and culture
technology and the potentials of bioprocess innovation were
published by a world-renowned scientific journal, Nature
Biotechnology.
*Ddobyul® is a registered trademark of Unhwa Corporation.
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15. Ddobyul® Plant Stem Cell
Published on Top
Academic Journal:
Nature Biotechnology
2010 November
Platform Technology
Patent Registered in
Major Countries
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16. Ddobyul® Plant Stem Cell
2011 Korea Technology Award Documentaries by
Discovery Channel
Green Certification & New Excellent
Coverage by all major
Technology Certification
media in Korea
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17. Ddobyul® Plant Stem Cell
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18. Ddobyul® Plant Stem Cell
Unhwa Corporation isolates stem cell from various plant species and conducts mass production to develop
pharmaceutical, nutrition, and cosmetic ingredient as well as botanical drug.
“To our knowledge, this is the firs time a stable plant stem cell line has been generated, constituting a significant milestone in biological
research. We consider this work to be cutting-edge research with enormous commercial potential.”
-Professor Gary Loake, Institute of Molecular Plant Sciences, University of Edinburgh
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19. Ddobyul® Plant Stem Cell
So far, Rare, Precious, or Endangered Plants are NEVER or SELDOM
been used for commercial products, although they are believed to
have the best efficacy…
50-year-old Wild Ginseng 1,000-year-old Yew Tree
(USD 100,000 / root) (Yew Tree lived from Tang-Dynasty)
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20. Ddobyul® Plant Stem Cell
But, we change it all…
50-year-old Wild Ginseng:
USD 100,000/root
Something only for Kings & Queens once per year…. …Now is ready for everybody’s daily consumption
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21. Ddobyul® Plant Stem Cell
Strong vitality Immune
?
enhancement
Anti-oxidation Anti-cancer
Anti- Anti-aging
inflammation
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22. Cosmetics Efficacy of Ddobyul® Plant Stem Cell
Efficacies
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23. Efficacy of Ddobyul® Plant Stem Cell
Boundless Vitality
• Ddobyul is pure plant stem cell that does not suffer from variation even after long period of culture. Ddobyul has a
strong vitality and survives various physical, chemical, and biological stress.
Survival in Freezing
Long-term culture
Different from common plant cells, Ddobyul survives extremely low temperature
Different from common plant cells, Ddobyul maintains the freezing condition, and perfectly re-grows after thawing, which enables the best
same stable biological and genetic states for long period of method for plant cell cryopreservation.
culturing.
Initial culture After 3 years Freezing Culture after Thawing
Callus Callus The cell is destroyed Rapidly dies after
Cell Cell by rigid cell wall. thawing.
The cell is Re-grows at the
Ddobyul Ddobyul preserved by same growth rate
flexible cell wall. before freezing.
Anti-oxidant Activity
• Generally, the cultured cells have higher anti-oxidant activity compared with other plant cells in order to prevent themselves from
being harmed by oxidant stresses of free radicals. Yew Ddobyul has 33.6 times higher CAT* activity than other cultured cell lines.
• Strong anti-oxidant activity keeps the human body healthy by eliminating the free radicals which cause many of the diseases.
- Antioxidant efficacy testing with yew Ddobyul -
※ CAT activity is an anti-oxidant activity
that serves an important role in
eliminating free radicals, and decrease in
CAT activity is correlated with
progression of Cancer cells (Breast
Cancer Research and treatment;2000)
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24. Efficacy of Ddobyul® Plant Stem Cell
Anti-cancer Activity
UHIST proved that yew Ddobyul is non-toxic and has a strong
anti-cancer effect through in-vivo & in-vitro experiments
Tumor volume
performed with the advice of Professor Ho-geun Lee of Jeonbuk
University.
• Anti-cancer in-vivo test w/ yew Ddobyul: Ddobyul treated (oral intake)
mice’s tumors weighted approximately 10 times less than the controlled
subjects. Tumor tissue examination results confirmed that Ddobyul cell
line induced cancer cell apoptosis and angiogenesis inhibition.
Control Ddobyul
• Anti-cancer in-vitro test w/ yew Ddobyul: Large intestine cancer, oral
cavity carcinoma, lung cancer, prostatic carcinoma, bone cancer,
Tumor weight
leukemia, uterine carcinoma, skin cancer, pancreas cancer, breast
cancer, stomach cancer, kidney cancer and liver cancer cells were tested
during in-vitro experiment. In comparison to the blockbuster drug
ingredient, paclitaxel, Ddobyul cells, which didn’t contain paclitaxel,
exhibited similar or more potent anti-cancer capabilities. Although
Ddobyul contains multiple compounds, its anti-cancerous suppressive power
was no less than paclitaxel, a single substance anti-cancer drug.
Control Ddobyul
In the experiment to cause inflammatory reaction onto HDF cells applying LPS, yew Ddobyul
methanol extract has shown strong anti-inflammatory activity by inhibiting expression of
inflammatory cytokines.
* Due to confidentiality, detailed data cannot be disclosed. Should you need additional information, please contact us (see page 17 for contact information).
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25. Efficacy of Ddobyul® Plant Stem Cell
Immune Enhancement Anti-aging Activity
Through research collaboration with professor Sang-yun Nam Through collaborative
of Jeonju University, Unhwa Biotech Corp. has found that the research with a cosmetics
wild ginseng Ddobyul greatly increases the activity of the NK company with R&D expertise,
cell*. It also induces bone marrow cell / lymph node cell Unhwa Biotech Corp. has
proliferation and helps boost the overall hematogenous verified that Ddobyul has an
function and immune system. outstanding efficacy on the
• After injecting an antigen, the mouse was supplemented with Ddobyul in a 1:20 skin. Ddobyul has anti-aging
ratio(free-choice feeding) for 10 days. The NK cell activity was approximately 1.5
times greater than the mouse that was not supplemented with Ddobyul.
effect to block the very
cause of aging.
• The mice supplemented with feed(free-choice feeding) mixed with Ddobyul had
almost twice the number of bone marrow cells compared with the mice that were
supplemented with only feed. The bone marrow cells produce the main compartments  The patent applied on wild ginseng
of blood; red blood cells, platelets etc. and can differentiate to immune cells; B cells, T derived plant stem cell’s efficacy of
cells etc. The bone marrow cell proliferation signifies the acceleration of the anti-aging and anti-oxidant (Application
hematogenous function and improvement of the immune system. number : 10-2008-0056008)
 The world’s first INCI (the International
• Cells of lymph node increased in the mice that were supplemented with the feed
Nomenclature of Cosmetic Ingredients)
mixed with Ddobyul. Most of cells in the lymph node are T cells and the increase of the
registration of plant stem cell and its
cells is the first response to antigen recognition. As antigen-specific T cells increase,
extract (June 2008)
other immune responses following T cell-increase are anticipated.
 Yew tree derived plant stem cell’s efficacy of anti-oxidant and anti-inflammatory (Application number : 10-2008-0044227)
Patents
 Wild ginseng derived plant stem cell’s efficacy of anti-aging and anti-oxidant (Application number : 10-2008-0056008)
 Yew tree derived plant stem cell’s efficacy of anti-cancer (Application number : 10-2008-0099718)
 Wild ginseng derived plant stem cell’s efficacy of anti-cancer (Application number : 10-2008-0110086)
 Wild ginseng derived plant stem cell’s efficacy of immune enhancement (Application number : 10-2009-0015040)
* Due to confidentiality, detailed data cannot be disclosed. Should you need additional information, please contact us.
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26. Efficacy of Ddobyul® Plant Stem Cell
Somatic cell-origin cell culture (A)
vs. Ddobyul cell culture (B)
Comparison of competitiveness of
Ddobyul cell culture technology A B
Previous cell culture technology Ddobyul cell culture technology
Genetic variation Variation No variation
Homogeneity Heterogeneous aggregated cell population Homogenous single cell population
Stability of Slow cell proliferation in continuous culture, Continuous, stable proliferation
proliferation gradual loss of cell line proliferation ability with a high proliferation rate
Productivity of useful Variation occurs, loss of productivity during Continuous maintenance of high productivity
material culture of useful materials without variation
Cell necrosis from severe aggregation and shear No cell necrosis from aggregation, strong
Cell necrosis
stress resistance to shear stress
Need of external
Requires continuous external material supply No need of continuous material supply
material supply
Resistance to Shear Resistant to stress and shock, proliferation
Easily damaged/necrotized from stress
stress and productivity are not affected
Survival rate of cell
10~20% 80~90%
cryopreservation
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27. Commercialization of Ddobyul Plant Stem Cell
Ddobyul from various plants possess diverse potentials which directly connect to five
different businesses.
Pharmaceutical
Anti-cancer
Efficacy Anti-oxidant
Health food
Anti-aging
Wild Ginseng
Anti-inflammatory
Cosmetic
Yew tree
Ddobyul
…
Mass production
Bioprocess Stable culture
Various innovation
Plant
species Cryopreservation
Cell line bank
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28. This is the beginning of a New Era
• Perfectly Safe
• Highly Effective
• Stable at Medical Level
• No pollution made
• Do not harm original plants
Welcome to the revolution brought by
Unhwa
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