Stimulus triggered fate conversion into pluripotency

1. Stimulus-triggered fate conversion of
somatic cells into pluripotency

2. Japanese research team discovered an unexpected
phenomenon of somatic cell reprogramming into pluripotent cells

3. Introduction
• Stem cells : ability to develop to any kind of cells
• Can be found in body or induced in laboratory

4. Why stem cells ?
• Cell-based Therapy .. Replace failed systems
• Research tool .. Study development
• resource for testing new medical treatments

5. Sources of stem cells
• Naturally :
When cells mature from embryo , some still immature
Bone marrow , blood , adipose tissue
• Induced :
• Transplantation of nuclei
• Modification of somatic cells
• Harsh conditions . . Acid bath

6. What is new in this research ?
• Researcher develop a way to generate SC* from adult cells ( acid bath )
• Easy
• Fast
• No risk

7. Designing the experiment
• Cell model : hematopoietic spleen (CD 45+)
• Animal model : mouse
• Conditions : acidic
• pluripotency markers : Oct 4
( Nanog, Sax2, Ecat1, Esg1, Dax1 )

8. No study so far has proven that such pluripotent cells can arise from
differentiated somatic cells

9. Results and discussion

18. Conclusion
This study show that when adult cells put in sub-lethal conditions it would not die ,
Instead it would converted to the un differentiated situation in which it Would
develop to any kind of cells

19. References
Haruko, Obokata, Wakayama Teruhiko, Kojima Koji, Vacanti Martin P, Niwa Hitoshi,
Yamato Masayuki, and Vacanti Charles A. "Stimulus-triggered Fate Conversion of
Somatic Cells into Pluripotency." Nature.com. Nature Publishing Group, 29 Jan.
2014. Web. 18 Mar. 2014.

20. Done by : Belal Abu Haneya
Hashemite University – Jordan
Supervisor : Dr. Abd Allatif Al Ghzawi
19 - 3 - 2014