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Minerick Final Presentation April 23rd 2010
- 1. Innovations in the Design and Detection of Polymer Lab-on-a-chip Microfluidic Devices Presented By: Aaron Ritthaler
- 2. Outline Background Introduction Experiments Results Conclusions References Questions
- 3. Background Microfluidic Devices serve many purposes Many advantages to this technology Limited by throughput and detection methodology
- 4. Literature Reviews High-throughput Lens-Free Imaging and Characterization of a Heterogeneous Cell Solution Polymer Lab-on-a-Chip System with Electrical Detection
- 5. High-throughput Lens-Free Imaging and Characterization of a Heterogeneous Cell Solution Goal: Replace Flow Cytometry with microfluidic detection device Built off of previous work (LUCAS) Focus on decision algorithm
- 6. Experiment Imaged polystyrene beads and a variety of cells No external fluid flow during imaging Depth of field was 4 mm Area of field was 10 cm2 “White” light source used to generate cell shadow images
- 7. Results Algorithm is limited by detection of overlapping cells and plane gaps Proven detection and separate characterization of three micro particles Methods developed to characterize undetectable cells
- 8. Polymer Lab-on-a-Chip System with Electrical Detection Goal: Present new materials and methods for Lab-on-a-chip devices Supports the use of PEEK for fabrication Demonstrates CCD (C4D) detection method Developed “MinCE”
- 9. Experiment Food and beverage analysis Biological analysis Medical analysis DNA applications
- 10. Results Successful in all experiments as a “proof of concept” Demonstrated advantages to using PEEK Proven use of CCD for DNA analysis
- 11. Conclusion New material and detection methods Expanded applications for devices Research shows promise for breaking limitations
- 12. References Ting-Wei Su, SungkyuSeo, Anthony Erlinger, AydoganOzcan, "High-Throughput Lensfree Imaging and Characterization of a Heterogeneous Cell Solution On a Chip", Biotechnology and Bioengineering 102 (2009) 856-868 Holger Muhlberger, Wonhee Hwang, Andreas E. Guber, Volker Saile, Werner Hoffmann, "Polymer Lab-on-a-Chip System with Electrical Detection", IEEE Sensors Journal, 8 (2008) 572-579
- 13. Questions?
Hinweis der Redaktion
- Purposes:-Point-of-care Medical Applications-Cell characterization-Disease Detection-Biological SeparationAdvantages:-Cost effective in a large variety of circumstances-Can operate with minimal support (all on-chip)-High surface-area-to-volume ration (catalysis)Limitations:-Field of View problems for detection-Usually require fluorescence-Decision Algorithms could be improved
- The proposed detection method was faster and cheaper than flow cytometry for characterizing micro particles.LUCAS = Lensless, Ultra-wide Cell Monitoring Array platform based on Shadow Imaging
- Equation was developed for characterizing overlapping cells, which wasn’t a problem below 50,000 cells/second flow. Plane gaps between cells were required to be 100 micrometer or greater.Proven for both Homogeneous mixtures of different sizes and heterogeneous mixtures.Methods developed include: Surface Chemistry, Dielectric Micro Bead, and Fluorescent particle labeling
- PEEK = Polyether Ether Ketone. Throughout the paper, they talk about the advantages and disadvantages of using PEEK, including the development of a new bonding process to overcome disadvantages. C4D = Capacitively Coupled Contactless Conductivity Measurements, which uses a signaling and detection electrode.MinCE = Completely Minimized Capillary Electrophoresis Device encompassing entire experimental apparatus in hand-held form.
- Food and Beverage – Characterized organic acids in wine and saccharides in fruit juices.Biological Analysis – Detection of 13 amino acids in acidic media.Medical Analysis – Detection of Lithium levels in blood, which Li+ is used as a treatment for manic-depressives.DNA Applications – Two separate DNA fragments were detected in electrophoresis gel medium.
- The use of PEEK, higher through-put designs, complex detection algorithms, and electrical detection methods opens the door for expanded applications. Applications include: DNA detection, Biological Analysis, Point-of-care Medical Analysis, and Food/Beverage Analysis to name a few.The literature shows that researchers are overstepping the limiting factors of microdevices. Expanded through-put and more complex detection methods are developing to create the next generation of microdevices.