1. Systematic reviews
in research using
animals; a policy
perspective.
Henk Reinen
Ingrid Hartgers

2. Animal tests in the Netherlands
1.800.000
Bron: Zo doende (NVWA)
1.600.000
1.400.000
1.200.000
Aantal dierproeven
1.000.000
800.000
600.000
400.000
200.000
0
1978 1980 1982 1984 1986 1988 1990 1992 1994 1996 1998 2000 2002 2004 2006 2008 2010
Jaar
2

3. Overview output Three R research
vaccine quality control RIVM/NVI
Test Animal model Three R method Status
Potency testing lethal challenge humane endpoints accepted Eur.Ph./WHO/FDA
(general)
100
Animal use in Bilthoven
Potency testing 90 lethal challenge serology accepted Eur.Ph./WHO
tetanus vaccine 80 mouse
70
Potency testing 60
lethal challenge serology accepted EurPh./WHO
diphteria vaccine guinea-pig
50
Potency testing 40 lethal challenge serology international validation
whole cell pertussis 30 study
20
Potency testing 10 multi-dose test one dose test accepted Eur.Ph./WHO
0
Safety diphtheria 1989 1991 1993 1995 1997 Vero cell test 2003 2005 2007 accepted Ph.Eur./WHO
dermal study in 1999 2001
vaccine guinea-pigs
3V-Platfom RIVM, 08-09-2011

4. Trend?
1.800.000
1.600.000
1.400.000
1.200.000
Aantal dierproeven
1.000.000
800.000
600.000
Refine
400.000
Reduce
200.000
Replace
€
0
1978 1980 1982 1984 1986 1988 1990 1992 1994 1996 1998 2000 2002 2004 2006 2008 2010
Jaar
Action
4
Plan

5. Action Plan
Sharing
Science Education
knowlegde
Collaboration EU, global Society
6

6. Pagina met twee tekstblokken
Directive Action Plan Alternatives
Protection of animals Transparancy Registrations Initiatives
Level playing field Monitoring “Stimulate” Knowledge
Project evaluation Registrations Facilitate Networks
=> Publications Regulate EU
authorisation Research Funds (ZonMW Global
7

7. Synthesis of evidence
Better science with less animals
Motion; standard in medical science,
should be common practice in science
using animals
Quality of publications
ZonMW , research
Thank you!
Raise support; awareness;
change of culture
Develop instruments, methodology,
guidelines, education
8