SlideShare ist ein Scribd-Unternehmen logo
1 von 1
Downloaden Sie, um offline zu lesen
Amplification of the No Template Control (NTC) due to Random
  Contamination - 3 Tips to avoid random contamination
  Occasionally NTC contamination randomly occurs when loading the DNA templates into the PCR plate. If the
  contamination is due to plate loading, the NTC will show amplification in some or all of the NTCs at varying
  CT values; see figure below.




      3 Tips to avoid random contamination:
            1. Use clean working practices to avoid template contamination.

            2. To avoid contamination from previous PCRs, incorporate an AmpEraseÒ Uracil N-Glycosylase (UNG)
               or Uracil-DNA Glycosylase (UDG) step prior to PCR to reduce carryover contamination.

            3. For optimal PCR results, use separate working areas for PCR mix preparation, template addition, and
               performing the PCR reactions.



      Have PCR questions? Ask them in the Life Technologies qPCR community.



      Continue troubleshooting - abnormal amplification with the Life Technologies gene expression –
      quantitation troubleshooting tool.



For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. © 2011 Life Technologies Corporation. All rights reserved. The
trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.

Weitere ähnliche Inhalte

Andere mochten auch

Dinsizliğin kabusu. turkish (türkçe)
Dinsizliğin kabusu. turkish (türkçe)Dinsizliğin kabusu. turkish (türkçe)
Dinsizliğin kabusu. turkish (türkçe)HarunyahyaTurkish
 
Killerspiele Kim Kleber Alex Kofler 1B
Killerspiele Kim Kleber Alex Kofler 1BKillerspiele Kim Kleber Alex Kofler 1B
Killerspiele Kim Kleber Alex Kofler 1BKim Kleber
 
North carolina State Business Email & Mailing List
North carolina  State Business Email & Mailing ListNorth carolina  State Business Email & Mailing List
North carolina State Business Email & Mailing ListGlobal B2B Contacts LLC
 
Para informatica (1)
Para informatica (1)Para informatica (1)
Para informatica (1)gustiadolfo
 
Evrimcilere net cevap 1. turkish (türkçe)
Evrimcilere net cevap 1. turkish (türkçe)Evrimcilere net cevap 1. turkish (türkçe)
Evrimcilere net cevap 1. turkish (türkçe)HarunyahyaTurkish
 
Convocação para o curso de formação
Convocação para o curso de formaçãoConvocação para o curso de formação
Convocação para o curso de formaçãoAspssauros Agentes
 
RIESGOS DE LA INTERNET
RIESGOS DE LA INTERNETRIESGOS DE LA INTERNET
RIESGOS DE LA INTERNETdora nubia
 
Darwinizm'in insanlığa getirdiği belalar. turkish (türkçe)
Darwinizm'in insanlığa getirdiği belalar. turkish (türkçe)Darwinizm'in insanlığa getirdiği belalar. turkish (türkçe)
Darwinizm'in insanlığa getirdiği belalar. turkish (türkçe)HarunyahyaTurkish
 
FlavorsCollectionbyAllenF.Stewart
FlavorsCollectionbyAllenF.StewartFlavorsCollectionbyAllenF.Stewart
FlavorsCollectionbyAllenF.StewartAllen Stewart
 
Darwin'i yıkan kafatasları. turkish (türkçe)
Darwin'i yıkan kafatasları. turkish (türkçe)Darwin'i yıkan kafatasları. turkish (türkçe)
Darwin'i yıkan kafatasları. turkish (türkçe)HarunyahyaTurkish
 
kuyper_instructionalscience29
kuyper_instructionalscience29kuyper_instructionalscience29
kuyper_instructionalscience29Michiel Kuijper
 
Alberto Pumarejo,Plan de gestión en TIC
Alberto Pumarejo,Plan de gestión en TICAlberto Pumarejo,Plan de gestión en TIC
Alberto Pumarejo,Plan de gestión en TICjaquicure
 

Andere mochten auch (16)

Dinsizliğin kabusu. turkish (türkçe)
Dinsizliğin kabusu. turkish (türkçe)Dinsizliğin kabusu. turkish (türkçe)
Dinsizliğin kabusu. turkish (türkçe)
 
Killerspiele Kim Kleber Alex Kofler 1B
Killerspiele Kim Kleber Alex Kofler 1BKillerspiele Kim Kleber Alex Kofler 1B
Killerspiele Kim Kleber Alex Kofler 1B
 
North carolina State Business Email & Mailing List
North carolina  State Business Email & Mailing ListNorth carolina  State Business Email & Mailing List
North carolina State Business Email & Mailing List
 
Para informatica (1)
Para informatica (1)Para informatica (1)
Para informatica (1)
 
Vernetzungstage 2011
Vernetzungstage 2011Vernetzungstage 2011
Vernetzungstage 2011
 
Evrimcilere net cevap 1. turkish (türkçe)
Evrimcilere net cevap 1. turkish (türkçe)Evrimcilere net cevap 1. turkish (türkçe)
Evrimcilere net cevap 1. turkish (türkçe)
 
Convocação para o curso de formação
Convocação para o curso de formaçãoConvocação para o curso de formação
Convocação para o curso de formação
 
RIESGOS DE LA INTERNET
RIESGOS DE LA INTERNETRIESGOS DE LA INTERNET
RIESGOS DE LA INTERNET
 
Final Draft
Final DraftFinal Draft
Final Draft
 
Darwinizm'in insanlığa getirdiği belalar. turkish (türkçe)
Darwinizm'in insanlığa getirdiği belalar. turkish (türkçe)Darwinizm'in insanlığa getirdiği belalar. turkish (türkçe)
Darwinizm'in insanlığa getirdiği belalar. turkish (türkçe)
 
FlavorsCollectionbyAllenF.Stewart
FlavorsCollectionbyAllenF.StewartFlavorsCollectionbyAllenF.Stewart
FlavorsCollectionbyAllenF.Stewart
 
Darwin'i yıkan kafatasları. turkish (türkçe)
Darwin'i yıkan kafatasları. turkish (türkçe)Darwin'i yıkan kafatasları. turkish (türkçe)
Darwin'i yıkan kafatasları. turkish (türkçe)
 
Welcome to Myanmar!
Welcome to Myanmar!Welcome to Myanmar!
Welcome to Myanmar!
 
Leaflet_MTX_1999_us
Leaflet_MTX_1999_usLeaflet_MTX_1999_us
Leaflet_MTX_1999_us
 
kuyper_instructionalscience29
kuyper_instructionalscience29kuyper_instructionalscience29
kuyper_instructionalscience29
 
Alberto Pumarejo,Plan de gestión en TIC
Alberto Pumarejo,Plan de gestión en TICAlberto Pumarejo,Plan de gestión en TIC
Alberto Pumarejo,Plan de gestión en TIC
 

Mehr von Thermo Fisher Scientific

Why you would want a powerful hot-start DNA polymerase for your PCR
Why you would want a powerful hot-start DNA polymerase for your PCRWhy you would want a powerful hot-start DNA polymerase for your PCR
Why you would want a powerful hot-start DNA polymerase for your PCRThermo Fisher Scientific
 
TCRB chain convergence in chronic cytomegalovirus infection and cancer
TCRB chain convergence in chronic cytomegalovirus infection and cancerTCRB chain convergence in chronic cytomegalovirus infection and cancer
TCRB chain convergence in chronic cytomegalovirus infection and cancerThermo Fisher Scientific
 
Improvement of TMB Measurement by removal of Deaminated Bases in FFPE DNA
Improvement of TMB Measurement by removal of Deaminated Bases in FFPE DNAImprovement of TMB Measurement by removal of Deaminated Bases in FFPE DNA
Improvement of TMB Measurement by removal of Deaminated Bases in FFPE DNAThermo Fisher Scientific
 
What can we learn from oncologists? A survey of molecular testing patterns
What can we learn from oncologists? A survey of molecular testing patternsWhat can we learn from oncologists? A survey of molecular testing patterns
What can we learn from oncologists? A survey of molecular testing patternsThermo Fisher Scientific
 
Evaluation of ctDNA extraction methods and amplifiable copy number yield usin...
Evaluation of ctDNA extraction methods and amplifiable copy number yield usin...Evaluation of ctDNA extraction methods and amplifiable copy number yield usin...
Evaluation of ctDNA extraction methods and amplifiable copy number yield usin...Thermo Fisher Scientific
 
Analytical Validation of the Oncomine™ Comprehensive Assay v3 with FFPE and C...
Analytical Validation of the Oncomine™ Comprehensive Assay v3 with FFPE and C...Analytical Validation of the Oncomine™ Comprehensive Assay v3 with FFPE and C...
Analytical Validation of the Oncomine™ Comprehensive Assay v3 with FFPE and C...Thermo Fisher Scientific
 
Novel Spatial Multiplex Screening of Uropathogens Associated with Urinary Tra...
Novel Spatial Multiplex Screening of Uropathogens Associated with Urinary Tra...Novel Spatial Multiplex Screening of Uropathogens Associated with Urinary Tra...
Novel Spatial Multiplex Screening of Uropathogens Associated with Urinary Tra...Thermo Fisher Scientific
 
Liquid biopsy quality control – the importance of plasma quality, sample prep...
Liquid biopsy quality control – the importance of plasma quality, sample prep...Liquid biopsy quality control – the importance of plasma quality, sample prep...
Liquid biopsy quality control – the importance of plasma quality, sample prep...Thermo Fisher Scientific
 
Streamlined next generation sequencing assay development using a highly multi...
Streamlined next generation sequencing assay development using a highly multi...Streamlined next generation sequencing assay development using a highly multi...
Streamlined next generation sequencing assay development using a highly multi...Thermo Fisher Scientific
 
Targeted T-cell receptor beta immune repertoire sequencing in several FFPE ti...
Targeted T-cell receptor beta immune repertoire sequencing in several FFPE ti...Targeted T-cell receptor beta immune repertoire sequencing in several FFPE ti...
Targeted T-cell receptor beta immune repertoire sequencing in several FFPE ti...Thermo Fisher Scientific
 
Development of Quality Control Materials for Characterization of Comprehensiv...
Development of Quality Control Materials for Characterization of Comprehensiv...Development of Quality Control Materials for Characterization of Comprehensiv...
Development of Quality Control Materials for Characterization of Comprehensiv...Thermo Fisher Scientific
 
A High Throughput System for Profiling Respiratory Tract Microbiota
A High Throughput System for Profiling Respiratory Tract MicrobiotaA High Throughput System for Profiling Respiratory Tract Microbiota
A High Throughput System for Profiling Respiratory Tract MicrobiotaThermo Fisher Scientific
 
A high-throughput approach for multi-omic testing for prostate cancer research
A high-throughput approach for multi-omic testing for prostate cancer researchA high-throughput approach for multi-omic testing for prostate cancer research
A high-throughput approach for multi-omic testing for prostate cancer researchThermo Fisher Scientific
 
Why is selecting the right thermal cycler important?
Why is selecting the right thermal cycler important?Why is selecting the right thermal cycler important?
Why is selecting the right thermal cycler important?Thermo Fisher Scientific
 
A rapid library preparation method with custom assay designs for detection of...
A rapid library preparation method with custom assay designs for detection of...A rapid library preparation method with custom assay designs for detection of...
A rapid library preparation method with custom assay designs for detection of...Thermo Fisher Scientific
 
Generation of Clonal CRISPR/Cas9-edited Human iPSC Derived Cellular Models an...
Generation of Clonal CRISPR/Cas9-edited Human iPSC Derived Cellular Models an...Generation of Clonal CRISPR/Cas9-edited Human iPSC Derived Cellular Models an...
Generation of Clonal CRISPR/Cas9-edited Human iPSC Derived Cellular Models an...Thermo Fisher Scientific
 
TaqMan®Advanced miRNA cDNA synthesis kit to simultaneously study expression o...
TaqMan®Advanced miRNA cDNA synthesis kit to simultaneously study expression o...TaqMan®Advanced miRNA cDNA synthesis kit to simultaneously study expression o...
TaqMan®Advanced miRNA cDNA synthesis kit to simultaneously study expression o...Thermo Fisher Scientific
 
Identifying novel and druggable targets in a triple negative breast cancer ce...
Identifying novel and druggable targets in a triple negative breast cancer ce...Identifying novel and druggable targets in a triple negative breast cancer ce...
Identifying novel and druggable targets in a triple negative breast cancer ce...Thermo Fisher Scientific
 
Evidence for antigen-driven TCRβ chain convergence in the melanoma-infiltrati...
Evidence for antigen-driven TCRβ chain convergence in the melanoma-infiltrati...Evidence for antigen-driven TCRβ chain convergence in the melanoma-infiltrati...
Evidence for antigen-driven TCRβ chain convergence in the melanoma-infiltrati...Thermo Fisher Scientific
 
Analytical performance of a novel next generation sequencing assay for Myeloi...
Analytical performance of a novel next generation sequencing assay for Myeloi...Analytical performance of a novel next generation sequencing assay for Myeloi...
Analytical performance of a novel next generation sequencing assay for Myeloi...Thermo Fisher Scientific
 

Mehr von Thermo Fisher Scientific (20)

Why you would want a powerful hot-start DNA polymerase for your PCR
Why you would want a powerful hot-start DNA polymerase for your PCRWhy you would want a powerful hot-start DNA polymerase for your PCR
Why you would want a powerful hot-start DNA polymerase for your PCR
 
TCRB chain convergence in chronic cytomegalovirus infection and cancer
TCRB chain convergence in chronic cytomegalovirus infection and cancerTCRB chain convergence in chronic cytomegalovirus infection and cancer
TCRB chain convergence in chronic cytomegalovirus infection and cancer
 
Improvement of TMB Measurement by removal of Deaminated Bases in FFPE DNA
Improvement of TMB Measurement by removal of Deaminated Bases in FFPE DNAImprovement of TMB Measurement by removal of Deaminated Bases in FFPE DNA
Improvement of TMB Measurement by removal of Deaminated Bases in FFPE DNA
 
What can we learn from oncologists? A survey of molecular testing patterns
What can we learn from oncologists? A survey of molecular testing patternsWhat can we learn from oncologists? A survey of molecular testing patterns
What can we learn from oncologists? A survey of molecular testing patterns
 
Evaluation of ctDNA extraction methods and amplifiable copy number yield usin...
Evaluation of ctDNA extraction methods and amplifiable copy number yield usin...Evaluation of ctDNA extraction methods and amplifiable copy number yield usin...
Evaluation of ctDNA extraction methods and amplifiable copy number yield usin...
 
Analytical Validation of the Oncomine™ Comprehensive Assay v3 with FFPE and C...
Analytical Validation of the Oncomine™ Comprehensive Assay v3 with FFPE and C...Analytical Validation of the Oncomine™ Comprehensive Assay v3 with FFPE and C...
Analytical Validation of the Oncomine™ Comprehensive Assay v3 with FFPE and C...
 
Novel Spatial Multiplex Screening of Uropathogens Associated with Urinary Tra...
Novel Spatial Multiplex Screening of Uropathogens Associated with Urinary Tra...Novel Spatial Multiplex Screening of Uropathogens Associated with Urinary Tra...
Novel Spatial Multiplex Screening of Uropathogens Associated with Urinary Tra...
 
Liquid biopsy quality control – the importance of plasma quality, sample prep...
Liquid biopsy quality control – the importance of plasma quality, sample prep...Liquid biopsy quality control – the importance of plasma quality, sample prep...
Liquid biopsy quality control – the importance of plasma quality, sample prep...
 
Streamlined next generation sequencing assay development using a highly multi...
Streamlined next generation sequencing assay development using a highly multi...Streamlined next generation sequencing assay development using a highly multi...
Streamlined next generation sequencing assay development using a highly multi...
 
Targeted T-cell receptor beta immune repertoire sequencing in several FFPE ti...
Targeted T-cell receptor beta immune repertoire sequencing in several FFPE ti...Targeted T-cell receptor beta immune repertoire sequencing in several FFPE ti...
Targeted T-cell receptor beta immune repertoire sequencing in several FFPE ti...
 
Development of Quality Control Materials for Characterization of Comprehensiv...
Development of Quality Control Materials for Characterization of Comprehensiv...Development of Quality Control Materials for Characterization of Comprehensiv...
Development of Quality Control Materials for Characterization of Comprehensiv...
 
A High Throughput System for Profiling Respiratory Tract Microbiota
A High Throughput System for Profiling Respiratory Tract MicrobiotaA High Throughput System for Profiling Respiratory Tract Microbiota
A High Throughput System for Profiling Respiratory Tract Microbiota
 
A high-throughput approach for multi-omic testing for prostate cancer research
A high-throughput approach for multi-omic testing for prostate cancer researchA high-throughput approach for multi-omic testing for prostate cancer research
A high-throughput approach for multi-omic testing for prostate cancer research
 
Why is selecting the right thermal cycler important?
Why is selecting the right thermal cycler important?Why is selecting the right thermal cycler important?
Why is selecting the right thermal cycler important?
 
A rapid library preparation method with custom assay designs for detection of...
A rapid library preparation method with custom assay designs for detection of...A rapid library preparation method with custom assay designs for detection of...
A rapid library preparation method with custom assay designs for detection of...
 
Generation of Clonal CRISPR/Cas9-edited Human iPSC Derived Cellular Models an...
Generation of Clonal CRISPR/Cas9-edited Human iPSC Derived Cellular Models an...Generation of Clonal CRISPR/Cas9-edited Human iPSC Derived Cellular Models an...
Generation of Clonal CRISPR/Cas9-edited Human iPSC Derived Cellular Models an...
 
TaqMan®Advanced miRNA cDNA synthesis kit to simultaneously study expression o...
TaqMan®Advanced miRNA cDNA synthesis kit to simultaneously study expression o...TaqMan®Advanced miRNA cDNA synthesis kit to simultaneously study expression o...
TaqMan®Advanced miRNA cDNA synthesis kit to simultaneously study expression o...
 
Identifying novel and druggable targets in a triple negative breast cancer ce...
Identifying novel and druggable targets in a triple negative breast cancer ce...Identifying novel and druggable targets in a triple negative breast cancer ce...
Identifying novel and druggable targets in a triple negative breast cancer ce...
 
Evidence for antigen-driven TCRβ chain convergence in the melanoma-infiltrati...
Evidence for antigen-driven TCRβ chain convergence in the melanoma-infiltrati...Evidence for antigen-driven TCRβ chain convergence in the melanoma-infiltrati...
Evidence for antigen-driven TCRβ chain convergence in the melanoma-infiltrati...
 
Analytical performance of a novel next generation sequencing assay for Myeloi...
Analytical performance of a novel next generation sequencing assay for Myeloi...Analytical performance of a novel next generation sequencing assay for Myeloi...
Analytical performance of a novel next generation sequencing assay for Myeloi...
 

Kürzlich hochgeladen

How to Add a New Field in Existing Kanban View in Odoo 17
How to Add a New Field in Existing Kanban View in Odoo 17How to Add a New Field in Existing Kanban View in Odoo 17
How to Add a New Field in Existing Kanban View in Odoo 17Celine George
 
Over the counter (OTC)- Sale, rational use.pptx
Over the counter (OTC)- Sale, rational use.pptxOver the counter (OTC)- Sale, rational use.pptx
Over the counter (OTC)- Sale, rational use.pptxraviapr7
 
5 charts on South Africa as a source country for international student recrui...
5 charts on South Africa as a source country for international student recrui...5 charts on South Africa as a source country for international student recrui...
5 charts on South Africa as a source country for international student recrui...CaraSkikne1
 
Department of Health Compounder Question ‍Solution 2022.pdf
Department of Health Compounder Question ‍Solution 2022.pdfDepartment of Health Compounder Question ‍Solution 2022.pdf
Department of Health Compounder Question ‍Solution 2022.pdfMohonDas
 
3.21.24 The Origins of Black Power.pptx
3.21.24  The Origins of Black Power.pptx3.21.24  The Origins of Black Power.pptx
3.21.24 The Origins of Black Power.pptxmary850239
 
CHUYÊN ĐỀ DẠY THÊM TIẾNG ANH LỚP 11 - GLOBAL SUCCESS - NĂM HỌC 2023-2024 - HK...
CHUYÊN ĐỀ DẠY THÊM TIẾNG ANH LỚP 11 - GLOBAL SUCCESS - NĂM HỌC 2023-2024 - HK...CHUYÊN ĐỀ DẠY THÊM TIẾNG ANH LỚP 11 - GLOBAL SUCCESS - NĂM HỌC 2023-2024 - HK...
CHUYÊN ĐỀ DẠY THÊM TIẾNG ANH LỚP 11 - GLOBAL SUCCESS - NĂM HỌC 2023-2024 - HK...Nguyen Thanh Tu Collection
 
10 Topics For MBA Project Report [HR].pdf
10 Topics For MBA Project Report [HR].pdf10 Topics For MBA Project Report [HR].pdf
10 Topics For MBA Project Report [HR].pdfJayanti Pande
 
Ultra structure and life cycle of Plasmodium.pptx
Ultra structure and life cycle of Plasmodium.pptxUltra structure and life cycle of Plasmodium.pptx
Ultra structure and life cycle of Plasmodium.pptxDr. Asif Anas
 
How to Show Error_Warning Messages in Odoo 17
How to Show Error_Warning Messages in Odoo 17How to Show Error_Warning Messages in Odoo 17
How to Show Error_Warning Messages in Odoo 17Celine George
 
2024.03.23 What do successful readers do - Sandy Millin for PARK.pptx
2024.03.23 What do successful readers do - Sandy Millin for PARK.pptx2024.03.23 What do successful readers do - Sandy Millin for PARK.pptx
2024.03.23 What do successful readers do - Sandy Millin for PARK.pptxSandy Millin
 
Diploma in Nursing Admission Test Question Solution 2023.pdf
Diploma in Nursing Admission Test Question Solution 2023.pdfDiploma in Nursing Admission Test Question Solution 2023.pdf
Diploma in Nursing Admission Test Question Solution 2023.pdfMohonDas
 
The Stolen Bacillus by Herbert George Wells
The Stolen Bacillus by Herbert George WellsThe Stolen Bacillus by Herbert George Wells
The Stolen Bacillus by Herbert George WellsEugene Lysak
 
SOLIDE WASTE in Cameroon,,,,,,,,,,,,,,,,,,,,,,,,,,,.pptx
SOLIDE WASTE in Cameroon,,,,,,,,,,,,,,,,,,,,,,,,,,,.pptxSOLIDE WASTE in Cameroon,,,,,,,,,,,,,,,,,,,,,,,,,,,.pptx
SOLIDE WASTE in Cameroon,,,,,,,,,,,,,,,,,,,,,,,,,,,.pptxSyedNadeemGillANi
 
3.26.24 Race, the Draft, and the Vietnam War.pptx
3.26.24 Race, the Draft, and the Vietnam War.pptx3.26.24 Race, the Draft, and the Vietnam War.pptx
3.26.24 Race, the Draft, and the Vietnam War.pptxmary850239
 
AUDIENCE THEORY -- FANDOM -- JENKINS.pptx
AUDIENCE THEORY -- FANDOM -- JENKINS.pptxAUDIENCE THEORY -- FANDOM -- JENKINS.pptx
AUDIENCE THEORY -- FANDOM -- JENKINS.pptxiammrhaywood
 
HED Office Sohayok Exam Question Solution 2023.pdf
HED Office Sohayok Exam Question Solution 2023.pdfHED Office Sohayok Exam Question Solution 2023.pdf
HED Office Sohayok Exam Question Solution 2023.pdfMohonDas
 
In - Vivo and In - Vitro Correlation.pptx
In - Vivo and In - Vitro Correlation.pptxIn - Vivo and In - Vitro Correlation.pptx
In - Vivo and In - Vitro Correlation.pptxAditiChauhan701637
 
Quality Assurance_GOOD LABORATORY PRACTICE
Quality Assurance_GOOD LABORATORY PRACTICEQuality Assurance_GOOD LABORATORY PRACTICE
Quality Assurance_GOOD LABORATORY PRACTICESayali Powar
 
Easter in the USA presentation by Chloe.
Easter in the USA presentation by Chloe.Easter in the USA presentation by Chloe.
Easter in the USA presentation by Chloe.EnglishCEIPdeSigeiro
 

Kürzlich hochgeladen (20)

March 2024 Directors Meeting, Division of Student Affairs and Academic Support
March 2024 Directors Meeting, Division of Student Affairs and Academic SupportMarch 2024 Directors Meeting, Division of Student Affairs and Academic Support
March 2024 Directors Meeting, Division of Student Affairs and Academic Support
 
How to Add a New Field in Existing Kanban View in Odoo 17
How to Add a New Field in Existing Kanban View in Odoo 17How to Add a New Field in Existing Kanban View in Odoo 17
How to Add a New Field in Existing Kanban View in Odoo 17
 
Over the counter (OTC)- Sale, rational use.pptx
Over the counter (OTC)- Sale, rational use.pptxOver the counter (OTC)- Sale, rational use.pptx
Over the counter (OTC)- Sale, rational use.pptx
 
5 charts on South Africa as a source country for international student recrui...
5 charts on South Africa as a source country for international student recrui...5 charts on South Africa as a source country for international student recrui...
5 charts on South Africa as a source country for international student recrui...
 
Department of Health Compounder Question ‍Solution 2022.pdf
Department of Health Compounder Question ‍Solution 2022.pdfDepartment of Health Compounder Question ‍Solution 2022.pdf
Department of Health Compounder Question ‍Solution 2022.pdf
 
3.21.24 The Origins of Black Power.pptx
3.21.24  The Origins of Black Power.pptx3.21.24  The Origins of Black Power.pptx
3.21.24 The Origins of Black Power.pptx
 
CHUYÊN ĐỀ DẠY THÊM TIẾNG ANH LỚP 11 - GLOBAL SUCCESS - NĂM HỌC 2023-2024 - HK...
CHUYÊN ĐỀ DẠY THÊM TIẾNG ANH LỚP 11 - GLOBAL SUCCESS - NĂM HỌC 2023-2024 - HK...CHUYÊN ĐỀ DẠY THÊM TIẾNG ANH LỚP 11 - GLOBAL SUCCESS - NĂM HỌC 2023-2024 - HK...
CHUYÊN ĐỀ DẠY THÊM TIẾNG ANH LỚP 11 - GLOBAL SUCCESS - NĂM HỌC 2023-2024 - HK...
 
10 Topics For MBA Project Report [HR].pdf
10 Topics For MBA Project Report [HR].pdf10 Topics For MBA Project Report [HR].pdf
10 Topics For MBA Project Report [HR].pdf
 
Ultra structure and life cycle of Plasmodium.pptx
Ultra structure and life cycle of Plasmodium.pptxUltra structure and life cycle of Plasmodium.pptx
Ultra structure and life cycle of Plasmodium.pptx
 
How to Show Error_Warning Messages in Odoo 17
How to Show Error_Warning Messages in Odoo 17How to Show Error_Warning Messages in Odoo 17
How to Show Error_Warning Messages in Odoo 17
 
2024.03.23 What do successful readers do - Sandy Millin for PARK.pptx
2024.03.23 What do successful readers do - Sandy Millin for PARK.pptx2024.03.23 What do successful readers do - Sandy Millin for PARK.pptx
2024.03.23 What do successful readers do - Sandy Millin for PARK.pptx
 
Diploma in Nursing Admission Test Question Solution 2023.pdf
Diploma in Nursing Admission Test Question Solution 2023.pdfDiploma in Nursing Admission Test Question Solution 2023.pdf
Diploma in Nursing Admission Test Question Solution 2023.pdf
 
The Stolen Bacillus by Herbert George Wells
The Stolen Bacillus by Herbert George WellsThe Stolen Bacillus by Herbert George Wells
The Stolen Bacillus by Herbert George Wells
 
SOLIDE WASTE in Cameroon,,,,,,,,,,,,,,,,,,,,,,,,,,,.pptx
SOLIDE WASTE in Cameroon,,,,,,,,,,,,,,,,,,,,,,,,,,,.pptxSOLIDE WASTE in Cameroon,,,,,,,,,,,,,,,,,,,,,,,,,,,.pptx
SOLIDE WASTE in Cameroon,,,,,,,,,,,,,,,,,,,,,,,,,,,.pptx
 
3.26.24 Race, the Draft, and the Vietnam War.pptx
3.26.24 Race, the Draft, and the Vietnam War.pptx3.26.24 Race, the Draft, and the Vietnam War.pptx
3.26.24 Race, the Draft, and the Vietnam War.pptx
 
AUDIENCE THEORY -- FANDOM -- JENKINS.pptx
AUDIENCE THEORY -- FANDOM -- JENKINS.pptxAUDIENCE THEORY -- FANDOM -- JENKINS.pptx
AUDIENCE THEORY -- FANDOM -- JENKINS.pptx
 
HED Office Sohayok Exam Question Solution 2023.pdf
HED Office Sohayok Exam Question Solution 2023.pdfHED Office Sohayok Exam Question Solution 2023.pdf
HED Office Sohayok Exam Question Solution 2023.pdf
 
In - Vivo and In - Vitro Correlation.pptx
In - Vivo and In - Vitro Correlation.pptxIn - Vivo and In - Vitro Correlation.pptx
In - Vivo and In - Vitro Correlation.pptx
 
Quality Assurance_GOOD LABORATORY PRACTICE
Quality Assurance_GOOD LABORATORY PRACTICEQuality Assurance_GOOD LABORATORY PRACTICE
Quality Assurance_GOOD LABORATORY PRACTICE
 
Easter in the USA presentation by Chloe.
Easter in the USA presentation by Chloe.Easter in the USA presentation by Chloe.
Easter in the USA presentation by Chloe.
 

Amplification of the No Template Control (NTC) - Tips to avoid random contamination

  • 1. Amplification of the No Template Control (NTC) due to Random Contamination - 3 Tips to avoid random contamination Occasionally NTC contamination randomly occurs when loading the DNA templates into the PCR plate. If the contamination is due to plate loading, the NTC will show amplification in some or all of the NTCs at varying CT values; see figure below. 3 Tips to avoid random contamination: 1. Use clean working practices to avoid template contamination. 2. To avoid contamination from previous PCRs, incorporate an AmpEraseÒ Uracil N-Glycosylase (UNG) or Uracil-DNA Glycosylase (UDG) step prior to PCR to reduce carryover contamination. 3. For optimal PCR results, use separate working areas for PCR mix preparation, template addition, and performing the PCR reactions. Have PCR questions? Ask them in the Life Technologies qPCR community. Continue troubleshooting - abnormal amplification with the Life Technologies gene expression – quantitation troubleshooting tool. For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. © 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.