1. Comparing Light and Electron
Microscopes
Advantages of Electron Disadvantages of Light
Microscopes Microscope
• Greater magnification up • Magnification only up to
to 1,000,000 x 1000-1,500 x
• Greater depth of field • Less depth of field
(With SEMs, 3-D ) • May involve complex
• No complex lighting lighting requirements
• Greater resolution -1 nm • Less resolution - 1 micron

2. Light Microscopes

3. Light microscope images
QuickTime™ and a
QuickTime™ and a
TIFF (Uncompressed) decompressor TIFF (Uncompressed) decompressor
are needed to see this picture. are needed to see this picture.
QuickTime™ and a QuickTime™ and a
TIFF (Uncompressed) decompressor
TIFF (Uncompressed) decompressor are needed to see this picture.
are needed to see this picture.

4. Microscopes Continued
Disadvantages of Advantages of Light
Electron Microscope Microscope
• Expensive (1 million) • Cheaper (100)
• Expensive to Run • Cheaper to Run
• Image Black and • Color image
White • Live or Dead
• Dead Specimen Specimen
• Requires Vacuum • No Vacuum Required
• Expert Preparation • Simple Preparation
• Special Large Room • Small and Portable
• Affected by Magnetic • Unaffected by
Fields Magnetic Fields

5. Electron Microscope images
QuickTime™ and a
TIFF (Uncompressed) decompressor
QuickTime™ and a are needed to see this picture.
TIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.

6. SEMs TEMs
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.

7. QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.

8. QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.

9. QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
40 x (SEM)
435x (SEM)
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.

10. Eye of a Mosquito
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.

11. QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
1760 x