SlideShare ist ein Scribd-Unternehmen logo
1 von 21
Downloaden Sie, um offline zu lesen
THE MICROSCOPE
Parts of the
light
microscope
Objective lenses
HP objective lens is
close to slide
Total magnification:
Magnification = Objective lens X Eyepiece lens
e.g. What is the total
magnification if the objective lens
is twenty times (X20) and the
eyepiece lens five times (X5)?
Magnification = 20 X 5 = X100
As magnification increases, detail
increases but:
Onion cell 40x
Onion cell 100x
Onion cell 400x
less of the cell is seen
Caring for the microscope:
1. Do not let any liquids to come in contact
with the microscope.
2. Always store the microscope inside a box
after use.
3. Return the objective lens onto low power
after use.
4. Carry the microscope by the arm.
5. Use a soft clean tissue to wipe the lenses
Microscope
slides
Coverslips
Fig. 2 Preparing
a slide as a wet
mount.
Use of stains:
 some parts of a plant cell can be clearly seen
when the cell is mounted in water
 E.g. an Elodea leaf cell:
 cell wall
 several chloroplasts
are seen
 other cell structures which are not so
obvious can often be shown up more clearly
by the addition of dyes called STAINS
METHYLENE BLUE
To stain animal cells
IODINE SOLUTION
To stain plant cells
A thin inner layer of
epidermis is peeled
off.
An onion is cut
into quarters.
One of the fleshy scale
leaves is removed.
Snapping leaf backwards
exposes the epidermis.
Epidermis is placed on
slide & covered with 2-3
drops of distilled water .
Coverslip is lowered.
A drop of stain is put at
one end of slide.
1 2
3
5
4
6
7
Stain is drawn over
specimen using a small
piece of filter paper.
Questions
1. A pupil prepared a microscope slide of onion
cells using water.
Diagram A shows how the cells looked when
first seen with the microscope.
Diagram B shows their appearance after the
addition of another liquid.
What name is given to a liquid used to make
cell structures easier to see?
Stain
2. The drawing shows the appearance of a
prepared slide.
What causes the ring shapes? (1)
Air bubbles trapped beneath the coverslip.
3. This diagram shows the main parts of a
typical light microscope.
a) How is a glass slide held in position on the
stage?
By the clips.
b) Why must the specimen on the slide be in
the centre of the hole in the stage?
To allow light pass through the specimen.
c) Why does the nosepiece rotate?
To clip in position the required objective lens.
d) What is the mirror for?
To reflect light onto the specimen.
e) How can you control the amount of light
coming through the microscope?
By moving the diaphragm.
f) Explain how you would use the microscope
to look at a specimen under the low power.
1. Clipping slide by clips.
2. Placing the low power objective in position.
3. Moving the tube downwards while looking
from the side until objective lens is close to
the slide.
4. Using the coarse adjustment
knob, the tube is racked
upwards until image is focused.
g) If you had a specimen in focus under the low
power, how would you go on to look at it
under high power?
By using the fine adjustment knob.
h) Why should you never rack downwards with
the coarse focusing knob while you are
looking down the microscope?
Not to break the slide.
i) If the magnifying power of the eyepiece lens
is ten times (X10), and that of the low power
objective lens is four times (X4), what is the
total magnification of a specimen under low
power?
10 x 4 = x40
T H E E N D

Weitere ähnliche Inhalte

Was ist angesagt?

compound microscope (basic)
compound microscope (basic)compound microscope (basic)
compound microscope (basic)Shruti Dhamdhere
 
History of the Microscope
History of the MicroscopeHistory of the Microscope
History of the Microscopeihmcbiology1213
 
Osmosis, diffusion, active transport
Osmosis, diffusion, active transportOsmosis, diffusion, active transport
Osmosis, diffusion, active transportMarthese Azzopardi
 
Microscope Biology I Lab
Microscope Biology I LabMicroscope Biology I Lab
Microscope Biology I LabLumen Learning
 
Levels of Organization (cell to organism)
Levels of Organization (cell to organism) Levels of Organization (cell to organism)
Levels of Organization (cell to organism) Melinda MacDonald
 
Introduction To Microscopes History & Parts
Introduction To  Microscopes    History &  PartsIntroduction To  Microscopes    History &  Parts
Introduction To Microscopes History & PartsMissWander
 
Microscope parts and functions
Microscope parts and functionsMicroscope parts and functions
Microscope parts and functionsjoevani_007
 
Transport in plants AS Biology [jm]
Transport in plants AS Biology  [jm]Transport in plants AS Biology  [jm]
Transport in plants AS Biology [jm]Jorge Pinto
 
Microscope presentation
Microscope presentationMicroscope presentation
Microscope presentationEiyla Akieym
 
Levels of organization
Levels of organizationLevels of organization
Levels of organizationTodd Bloch
 
Types of cells notes ppt
Types of cells notes   pptTypes of cells notes   ppt
Types of cells notes pptKaren Kuo
 
Sexual versus asexual reproduction
Sexual versus asexual reproductionSexual versus asexual reproduction
Sexual versus asexual reproductionBokadrum
 

Was ist angesagt? (20)

compound microscope (basic)
compound microscope (basic)compound microscope (basic)
compound microscope (basic)
 
Types of Microscope
Types of MicroscopeTypes of Microscope
Types of Microscope
 
History of the Microscope
History of the MicroscopeHistory of the Microscope
History of the Microscope
 
Osmosis, diffusion, active transport
Osmosis, diffusion, active transportOsmosis, diffusion, active transport
Osmosis, diffusion, active transport
 
Parts of the Cell
Parts of the CellParts of the Cell
Parts of the Cell
 
Microscope Biology I Lab
Microscope Biology I LabMicroscope Biology I Lab
Microscope Biology I Lab
 
The microscope
The microscopeThe microscope
The microscope
 
Dichotomous key
Dichotomous keyDichotomous key
Dichotomous key
 
Microscope powerpoint
Microscope powerpointMicroscope powerpoint
Microscope powerpoint
 
Levels of Organization (cell to organism)
Levels of Organization (cell to organism) Levels of Organization (cell to organism)
Levels of Organization (cell to organism)
 
Introduction To Microscopes History & Parts
Introduction To  Microscopes    History &  PartsIntroduction To  Microscopes    History &  Parts
Introduction To Microscopes History & Parts
 
microscope
microscopemicroscope
microscope
 
Microscope parts and functions
Microscope parts and functionsMicroscope parts and functions
Microscope parts and functions
 
Transport in plants AS Biology [jm]
Transport in plants AS Biology  [jm]Transport in plants AS Biology  [jm]
Transport in plants AS Biology [jm]
 
Microscope presentation
Microscope presentationMicroscope presentation
Microscope presentation
 
Levels of organization
Levels of organizationLevels of organization
Levels of organization
 
Types of cells notes ppt
Types of cells notes   pptTypes of cells notes   ppt
Types of cells notes ppt
 
Onion cells
Onion cellsOnion cells
Onion cells
 
Microscope
MicroscopeMicroscope
Microscope
 
Sexual versus asexual reproduction
Sexual versus asexual reproductionSexual versus asexual reproduction
Sexual versus asexual reproduction
 

Andere mochten auch

Lesson Plan and Worksheets on Characteristics of Living Lhings
Lesson Plan and Worksheets on Characteristics of Living LhingsLesson Plan and Worksheets on Characteristics of Living Lhings
Lesson Plan and Worksheets on Characteristics of Living LhingsKavita Grover
 
Sunkenalive7
Sunkenalive7Sunkenalive7
Sunkenalive7lesberg1
 
cell the unit of life
cell the unit of lifecell the unit of life
cell the unit of lifeVinay Patil
 
Igcse biology edexcel 1.1 1.3
Igcse biology edexcel 1.1 1.3Igcse biology edexcel 1.1 1.3
Igcse biology edexcel 1.1 1.3Marc Rodriguez
 
Characteristics of living things
Characteristics of living thingsCharacteristics of living things
Characteristics of living thingssamuelchoi28
 
Characteristics of living things
Characteristics of living thingsCharacteristics of living things
Characteristics of living thingsJenny Dixon
 
Preparing a cheek cell slide
Preparing a cheek cell slidePreparing a cheek cell slide
Preparing a cheek cell slideAnisa Farah
 
Mrs.Gren
Mrs.GrenMrs.Gren
Mrs.GrenLM9
 

Andere mochten auch (10)

Lesson Plan and Worksheets on Characteristics of Living Lhings
Lesson Plan and Worksheets on Characteristics of Living LhingsLesson Plan and Worksheets on Characteristics of Living Lhings
Lesson Plan and Worksheets on Characteristics of Living Lhings
 
Sunkenalive7
Sunkenalive7Sunkenalive7
Sunkenalive7
 
cell the unit of life
cell the unit of lifecell the unit of life
cell the unit of life
 
Igcse biology edexcel 1.1 1.3
Igcse biology edexcel 1.1 1.3Igcse biology edexcel 1.1 1.3
Igcse biology edexcel 1.1 1.3
 
Procedure
ProcedureProcedure
Procedure
 
Characteristics of living things
Characteristics of living thingsCharacteristics of living things
Characteristics of living things
 
Characteristics of living things
Characteristics of living thingsCharacteristics of living things
Characteristics of living things
 
Preparing a cheek cell slide
Preparing a cheek cell slidePreparing a cheek cell slide
Preparing a cheek cell slide
 
S2b445
S2b445S2b445
S2b445
 
Mrs.Gren
Mrs.GrenMrs.Gren
Mrs.Gren
 

Ähnlich wie Microscope

Microscopy Is A Practical Field For Using Microscope
Microscopy Is A Practical Field For Using MicroscopeMicroscopy Is A Practical Field For Using Microscope
Microscopy Is A Practical Field For Using MicroscopeMelissa Dudas
 
Chapter 2 section 2 (2011): Viewing Cells
Chapter 2 section 2 (2011):  Viewing CellsChapter 2 section 2 (2011):  Viewing Cells
Chapter 2 section 2 (2011): Viewing CellsMr. Motuk
 
Q2_living things and their environment.pptx
Q2_living things and their environment.pptxQ2_living things and their environment.pptx
Q2_living things and their environment.pptxAURORACARIAGA2
 
PP3_History_of_microscopy_1453876435.ppt
PP3_History_of_microscopy_1453876435.pptPP3_History_of_microscopy_1453876435.ppt
PP3_History_of_microscopy_1453876435.pptMuhammad Rawat
 
Microbiology1 cells & processes new
Microbiology1 cells & processes newMicrobiology1 cells & processes new
Microbiology1 cells & processes newRobin Seamon
 
Science Fair Project Research Report
Science Fair Project Research ReportScience Fair Project Research Report
Science Fair Project Research ReportAlison Hall
 
To study working of compound microscope .docx
To study working of compound microscope .docxTo study working of compound microscope .docx
To study working of compound microscope .docxMsNehaSharma2
 
Microscope ...chanderhash..asu2017010100041
Microscope ...chanderhash..asu2017010100041Microscope ...chanderhash..asu2017010100041
Microscope ...chanderhash..asu2017010100041chanderhash kumar
 
Week 08 - Leading for ChangeFollowership Objecti.docx
Week 08 - Leading for ChangeFollowership Objecti.docxWeek 08 - Leading for ChangeFollowership Objecti.docx
Week 08 - Leading for ChangeFollowership Objecti.docxcelenarouzie
 
Chapter 2 section 2 (2011)
Chapter 2 section 2 (2011)Chapter 2 section 2 (2011)
Chapter 2 section 2 (2011)Mr. Motuk
 
Microbiology1 cells & processes new
Microbiology1 cells & processes newMicrobiology1 cells & processes new
Microbiology1 cells & processes newRobin Seamon
 
Microbiology1 cells & processes
Microbiology1 cells & processesMicrobiology1 cells & processes
Microbiology1 cells & processesRobin Seamon
 
preparing-onion-cell-slides-lesson-element-teacher-instructions
preparing-onion-cell-slides-lesson-element-teacher-instructionspreparing-onion-cell-slides-lesson-element-teacher-instructions
preparing-onion-cell-slides-lesson-element-teacher-instructionsVasiliki Makrygianni
 
Microscope Notes- Types & Use.ppt.pdf
Microscope Notes- Types & Use.ppt.pdfMicroscope Notes- Types & Use.ppt.pdf
Microscope Notes- Types & Use.ppt.pdfMunishGuleria4
 
Microbiology lab 2
Microbiology lab 2Microbiology lab 2
Microbiology lab 2MBBS IMS MSU
 
Microbiology lab 2
Microbiology lab 2Microbiology lab 2
Microbiology lab 2MBBS IMS MSU
 
Microbial Survey, Smear Preparation, And Simple Stain Essay
Microbial Survey, Smear Preparation, And Simple Stain EssayMicrobial Survey, Smear Preparation, And Simple Stain Essay
Microbial Survey, Smear Preparation, And Simple Stain EssayAliyahh King
 
1. Grade-7-Q2-Module-1-Parts-and-Functions-of-the-Compound-Microscope-and-its...
1. Grade-7-Q2-Module-1-Parts-and-Functions-of-the-Compound-Microscope-and-its...1. Grade-7-Q2-Module-1-Parts-and-Functions-of-the-Compound-Microscope-and-its...
1. Grade-7-Q2-Module-1-Parts-and-Functions-of-the-Compound-Microscope-and-its...rosepalmajalandoni
 

Ähnlich wie Microscope (20)

Microscopy Is A Practical Field For Using Microscope
Microscopy Is A Practical Field For Using MicroscopeMicroscopy Is A Practical Field For Using Microscope
Microscopy Is A Practical Field For Using Microscope
 
Chapter 2 section 2 (2011): Viewing Cells
Chapter 2 section 2 (2011):  Viewing CellsChapter 2 section 2 (2011):  Viewing Cells
Chapter 2 section 2 (2011): Viewing Cells
 
Q2_living things and their environment.pptx
Q2_living things and their environment.pptxQ2_living things and their environment.pptx
Q2_living things and their environment.pptx
 
PP3_History_of_microscopy_1453876435.ppt
PP3_History_of_microscopy_1453876435.pptPP3_History_of_microscopy_1453876435.ppt
PP3_History_of_microscopy_1453876435.ppt
 
Microbiology1 cells & processes new
Microbiology1 cells & processes newMicrobiology1 cells & processes new
Microbiology1 cells & processes new
 
Science Fair Project Research Report
Science Fair Project Research ReportScience Fair Project Research Report
Science Fair Project Research Report
 
To study working of compound microscope .docx
To study working of compound microscope .docxTo study working of compound microscope .docx
To study working of compound microscope .docx
 
Microscope ...chanderhash..asu2017010100041
Microscope ...chanderhash..asu2017010100041Microscope ...chanderhash..asu2017010100041
Microscope ...chanderhash..asu2017010100041
 
Week 08 - Leading for ChangeFollowership Objecti.docx
Week 08 - Leading for ChangeFollowership Objecti.docxWeek 08 - Leading for ChangeFollowership Objecti.docx
Week 08 - Leading for ChangeFollowership Objecti.docx
 
LAB M1.pptx
LAB M1.pptxLAB M1.pptx
LAB M1.pptx
 
Chapter 2 section 2 (2011)
Chapter 2 section 2 (2011)Chapter 2 section 2 (2011)
Chapter 2 section 2 (2011)
 
Microbiology1 cells & processes new
Microbiology1 cells & processes newMicrobiology1 cells & processes new
Microbiology1 cells & processes new
 
Microbiology1 cells & processes
Microbiology1 cells & processesMicrobiology1 cells & processes
Microbiology1 cells & processes
 
Pond Water Essay
Pond Water EssayPond Water Essay
Pond Water Essay
 
preparing-onion-cell-slides-lesson-element-teacher-instructions
preparing-onion-cell-slides-lesson-element-teacher-instructionspreparing-onion-cell-slides-lesson-element-teacher-instructions
preparing-onion-cell-slides-lesson-element-teacher-instructions
 
Microscope Notes- Types & Use.ppt.pdf
Microscope Notes- Types & Use.ppt.pdfMicroscope Notes- Types & Use.ppt.pdf
Microscope Notes- Types & Use.ppt.pdf
 
Microbiology lab 2
Microbiology lab 2Microbiology lab 2
Microbiology lab 2
 
Microbiology lab 2
Microbiology lab 2Microbiology lab 2
Microbiology lab 2
 
Microbial Survey, Smear Preparation, And Simple Stain Essay
Microbial Survey, Smear Preparation, And Simple Stain EssayMicrobial Survey, Smear Preparation, And Simple Stain Essay
Microbial Survey, Smear Preparation, And Simple Stain Essay
 
1. Grade-7-Q2-Module-1-Parts-and-Functions-of-the-Compound-Microscope-and-its...
1. Grade-7-Q2-Module-1-Parts-and-Functions-of-the-Compound-Microscope-and-its...1. Grade-7-Q2-Module-1-Parts-and-Functions-of-the-Compound-Microscope-and-its...
1. Grade-7-Q2-Module-1-Parts-and-Functions-of-the-Compound-Microscope-and-its...
 

Mehr von Marthese Azzopardi (20)

Tropisms
TropismsTropisms
Tropisms
 
Environmental biology
Environmental biologyEnvironmental biology
Environmental biology
 
Soil
SoilSoil
Soil
 
Genetics
GeneticsGenetics
Genetics
 
Circulation
CirculationCirculation
Circulation
 
Fungi kingdom
Fungi kingdomFungi kingdom
Fungi kingdom
 
Bacteria & viruses
Bacteria & virusesBacteria & viruses
Bacteria & viruses
 
Ecology
EcologyEcology
Ecology
 
Tapeworm
TapewormTapeworm
Tapeworm
 
Digestion
DigestionDigestion
Digestion
 
Teeth
TeethTeeth
Teeth
 
Food and diet
Food and dietFood and diet
Food and diet
 
Co ordination
Co ordinationCo ordination
Co ordination
 
Kidneys
KidneysKidneys
Kidneys
 
Leaf structure
Leaf structureLeaf structure
Leaf structure
 
Photosynthesis
PhotosynthesisPhotosynthesis
Photosynthesis
 
Respiratory system
Respiratory systemRespiratory system
Respiratory system
 
Respiration
RespirationRespiration
Respiration
 
Amoeba & chlorella
Amoeba & chlorellaAmoeba & chlorella
Amoeba & chlorella
 
Cells
CellsCells
Cells
 

Kürzlich hochgeladen

Building Your Own AI Instance (TBLC AI )
Building Your Own AI Instance (TBLC AI )Building Your Own AI Instance (TBLC AI )
Building Your Own AI Instance (TBLC AI )Brian Pichman
 
Videogame localization & technology_ how to enhance the power of translation.pdf
Videogame localization & technology_ how to enhance the power of translation.pdfVideogame localization & technology_ how to enhance the power of translation.pdf
Videogame localization & technology_ how to enhance the power of translation.pdfinfogdgmi
 
9 Steps For Building Winning Founding Team
9 Steps For Building Winning Founding Team9 Steps For Building Winning Founding Team
9 Steps For Building Winning Founding TeamAdam Moalla
 
Meet the new FSP 3000 M-Flex800™
Meet the new FSP 3000 M-Flex800™Meet the new FSP 3000 M-Flex800™
Meet the new FSP 3000 M-Flex800™Adtran
 
Designing A Time bound resource download URL
Designing A Time bound resource download URLDesigning A Time bound resource download URL
Designing A Time bound resource download URLRuncy Oommen
 
Anypoint Code Builder , Google Pub sub connector and MuleSoft RPA
Anypoint Code Builder , Google Pub sub connector and MuleSoft RPAAnypoint Code Builder , Google Pub sub connector and MuleSoft RPA
Anypoint Code Builder , Google Pub sub connector and MuleSoft RPAshyamraj55
 
Comparing Sidecar-less Service Mesh from Cilium and Istio
Comparing Sidecar-less Service Mesh from Cilium and IstioComparing Sidecar-less Service Mesh from Cilium and Istio
Comparing Sidecar-less Service Mesh from Cilium and IstioChristian Posta
 
UiPath Community: AI for UiPath Automation Developers
UiPath Community: AI for UiPath Automation DevelopersUiPath Community: AI for UiPath Automation Developers
UiPath Community: AI for UiPath Automation DevelopersUiPathCommunity
 
Building AI-Driven Apps Using Semantic Kernel.pptx
Building AI-Driven Apps Using Semantic Kernel.pptxBuilding AI-Driven Apps Using Semantic Kernel.pptx
Building AI-Driven Apps Using Semantic Kernel.pptxUdaiappa Ramachandran
 
Secure your environment with UiPath and CyberArk technologies - Session 1
Secure your environment with UiPath and CyberArk technologies - Session 1Secure your environment with UiPath and CyberArk technologies - Session 1
Secure your environment with UiPath and CyberArk technologies - Session 1DianaGray10
 
How Accurate are Carbon Emissions Projections?
How Accurate are Carbon Emissions Projections?How Accurate are Carbon Emissions Projections?
How Accurate are Carbon Emissions Projections?IES VE
 
UiPath Platform: The Backend Engine Powering Your Automation - Session 1
UiPath Platform: The Backend Engine Powering Your Automation - Session 1UiPath Platform: The Backend Engine Powering Your Automation - Session 1
UiPath Platform: The Backend Engine Powering Your Automation - Session 1DianaGray10
 
Machine Learning Model Validation (Aijun Zhang 2024).pdf
Machine Learning Model Validation (Aijun Zhang 2024).pdfMachine Learning Model Validation (Aijun Zhang 2024).pdf
Machine Learning Model Validation (Aijun Zhang 2024).pdfAijun Zhang
 
AI You Can Trust - Ensuring Success with Data Integrity Webinar
AI You Can Trust - Ensuring Success with Data Integrity WebinarAI You Can Trust - Ensuring Success with Data Integrity Webinar
AI You Can Trust - Ensuring Success with Data Integrity WebinarPrecisely
 
Salesforce Miami User Group Event - 1st Quarter 2024
Salesforce Miami User Group Event - 1st Quarter 2024Salesforce Miami User Group Event - 1st Quarter 2024
Salesforce Miami User Group Event - 1st Quarter 2024SkyPlanner
 
VoIP Service and Marketing using Odoo and Asterisk PBX
VoIP Service and Marketing using Odoo and Asterisk PBXVoIP Service and Marketing using Odoo and Asterisk PBX
VoIP Service and Marketing using Odoo and Asterisk PBXTarek Kalaji
 
UiPath Studio Web workshop series - Day 8
UiPath Studio Web workshop series - Day 8UiPath Studio Web workshop series - Day 8
UiPath Studio Web workshop series - Day 8DianaGray10
 
Apres-Cyber - The Data Dilemma: Bridging Offensive Operations and Machine Lea...
Apres-Cyber - The Data Dilemma: Bridging Offensive Operations and Machine Lea...Apres-Cyber - The Data Dilemma: Bridging Offensive Operations and Machine Lea...
Apres-Cyber - The Data Dilemma: Bridging Offensive Operations and Machine Lea...Will Schroeder
 
Nanopower In Semiconductor Industry.pdf
Nanopower  In Semiconductor Industry.pdfNanopower  In Semiconductor Industry.pdf
Nanopower In Semiconductor Industry.pdfPedro Manuel
 
Computer 10: Lesson 10 - Online Crimes and Hazards
Computer 10: Lesson 10 - Online Crimes and HazardsComputer 10: Lesson 10 - Online Crimes and Hazards
Computer 10: Lesson 10 - Online Crimes and HazardsSeth Reyes
 

Kürzlich hochgeladen (20)

Building Your Own AI Instance (TBLC AI )
Building Your Own AI Instance (TBLC AI )Building Your Own AI Instance (TBLC AI )
Building Your Own AI Instance (TBLC AI )
 
Videogame localization & technology_ how to enhance the power of translation.pdf
Videogame localization & technology_ how to enhance the power of translation.pdfVideogame localization & technology_ how to enhance the power of translation.pdf
Videogame localization & technology_ how to enhance the power of translation.pdf
 
9 Steps For Building Winning Founding Team
9 Steps For Building Winning Founding Team9 Steps For Building Winning Founding Team
9 Steps For Building Winning Founding Team
 
Meet the new FSP 3000 M-Flex800™
Meet the new FSP 3000 M-Flex800™Meet the new FSP 3000 M-Flex800™
Meet the new FSP 3000 M-Flex800™
 
Designing A Time bound resource download URL
Designing A Time bound resource download URLDesigning A Time bound resource download URL
Designing A Time bound resource download URL
 
Anypoint Code Builder , Google Pub sub connector and MuleSoft RPA
Anypoint Code Builder , Google Pub sub connector and MuleSoft RPAAnypoint Code Builder , Google Pub sub connector and MuleSoft RPA
Anypoint Code Builder , Google Pub sub connector and MuleSoft RPA
 
Comparing Sidecar-less Service Mesh from Cilium and Istio
Comparing Sidecar-less Service Mesh from Cilium and IstioComparing Sidecar-less Service Mesh from Cilium and Istio
Comparing Sidecar-less Service Mesh from Cilium and Istio
 
UiPath Community: AI for UiPath Automation Developers
UiPath Community: AI for UiPath Automation DevelopersUiPath Community: AI for UiPath Automation Developers
UiPath Community: AI for UiPath Automation Developers
 
Building AI-Driven Apps Using Semantic Kernel.pptx
Building AI-Driven Apps Using Semantic Kernel.pptxBuilding AI-Driven Apps Using Semantic Kernel.pptx
Building AI-Driven Apps Using Semantic Kernel.pptx
 
Secure your environment with UiPath and CyberArk technologies - Session 1
Secure your environment with UiPath and CyberArk technologies - Session 1Secure your environment with UiPath and CyberArk technologies - Session 1
Secure your environment with UiPath and CyberArk technologies - Session 1
 
How Accurate are Carbon Emissions Projections?
How Accurate are Carbon Emissions Projections?How Accurate are Carbon Emissions Projections?
How Accurate are Carbon Emissions Projections?
 
UiPath Platform: The Backend Engine Powering Your Automation - Session 1
UiPath Platform: The Backend Engine Powering Your Automation - Session 1UiPath Platform: The Backend Engine Powering Your Automation - Session 1
UiPath Platform: The Backend Engine Powering Your Automation - Session 1
 
Machine Learning Model Validation (Aijun Zhang 2024).pdf
Machine Learning Model Validation (Aijun Zhang 2024).pdfMachine Learning Model Validation (Aijun Zhang 2024).pdf
Machine Learning Model Validation (Aijun Zhang 2024).pdf
 
AI You Can Trust - Ensuring Success with Data Integrity Webinar
AI You Can Trust - Ensuring Success with Data Integrity WebinarAI You Can Trust - Ensuring Success with Data Integrity Webinar
AI You Can Trust - Ensuring Success with Data Integrity Webinar
 
Salesforce Miami User Group Event - 1st Quarter 2024
Salesforce Miami User Group Event - 1st Quarter 2024Salesforce Miami User Group Event - 1st Quarter 2024
Salesforce Miami User Group Event - 1st Quarter 2024
 
VoIP Service and Marketing using Odoo and Asterisk PBX
VoIP Service and Marketing using Odoo and Asterisk PBXVoIP Service and Marketing using Odoo and Asterisk PBX
VoIP Service and Marketing using Odoo and Asterisk PBX
 
UiPath Studio Web workshop series - Day 8
UiPath Studio Web workshop series - Day 8UiPath Studio Web workshop series - Day 8
UiPath Studio Web workshop series - Day 8
 
Apres-Cyber - The Data Dilemma: Bridging Offensive Operations and Machine Lea...
Apres-Cyber - The Data Dilemma: Bridging Offensive Operations and Machine Lea...Apres-Cyber - The Data Dilemma: Bridging Offensive Operations and Machine Lea...
Apres-Cyber - The Data Dilemma: Bridging Offensive Operations and Machine Lea...
 
Nanopower In Semiconductor Industry.pdf
Nanopower  In Semiconductor Industry.pdfNanopower  In Semiconductor Industry.pdf
Nanopower In Semiconductor Industry.pdf
 
Computer 10: Lesson 10 - Online Crimes and Hazards
Computer 10: Lesson 10 - Online Crimes and HazardsComputer 10: Lesson 10 - Online Crimes and Hazards
Computer 10: Lesson 10 - Online Crimes and Hazards
 

Microscope

  • 3. Objective lenses HP objective lens is close to slide
  • 4. Total magnification: Magnification = Objective lens X Eyepiece lens e.g. What is the total magnification if the objective lens is twenty times (X20) and the eyepiece lens five times (X5)? Magnification = 20 X 5 = X100
  • 5. As magnification increases, detail increases but: Onion cell 40x Onion cell 100x Onion cell 400x less of the cell is seen
  • 6. Caring for the microscope: 1. Do not let any liquids to come in contact with the microscope. 2. Always store the microscope inside a box after use. 3. Return the objective lens onto low power after use. 4. Carry the microscope by the arm. 5. Use a soft clean tissue to wipe the lenses
  • 8. Fig. 2 Preparing a slide as a wet mount.
  • 9. Use of stains:  some parts of a plant cell can be clearly seen when the cell is mounted in water  E.g. an Elodea leaf cell:  cell wall  several chloroplasts are seen
  • 10.  other cell structures which are not so obvious can often be shown up more clearly by the addition of dyes called STAINS METHYLENE BLUE To stain animal cells IODINE SOLUTION To stain plant cells
  • 11. A thin inner layer of epidermis is peeled off. An onion is cut into quarters. One of the fleshy scale leaves is removed. Snapping leaf backwards exposes the epidermis. Epidermis is placed on slide & covered with 2-3 drops of distilled water . Coverslip is lowered. A drop of stain is put at one end of slide. 1 2 3 5 4 6 7 Stain is drawn over specimen using a small piece of filter paper.
  • 12. Questions 1. A pupil prepared a microscope slide of onion cells using water. Diagram A shows how the cells looked when first seen with the microscope. Diagram B shows their appearance after the addition of another liquid. What name is given to a liquid used to make cell structures easier to see? Stain
  • 13. 2. The drawing shows the appearance of a prepared slide. What causes the ring shapes? (1) Air bubbles trapped beneath the coverslip.
  • 14. 3. This diagram shows the main parts of a typical light microscope. a) How is a glass slide held in position on the stage? By the clips.
  • 15. b) Why must the specimen on the slide be in the centre of the hole in the stage? To allow light pass through the specimen. c) Why does the nosepiece rotate? To clip in position the required objective lens.
  • 16. d) What is the mirror for? To reflect light onto the specimen. e) How can you control the amount of light coming through the microscope? By moving the diaphragm.
  • 17. f) Explain how you would use the microscope to look at a specimen under the low power. 1. Clipping slide by clips. 2. Placing the low power objective in position. 3. Moving the tube downwards while looking from the side until objective lens is close to the slide. 4. Using the coarse adjustment knob, the tube is racked upwards until image is focused.
  • 18. g) If you had a specimen in focus under the low power, how would you go on to look at it under high power? By using the fine adjustment knob.
  • 19. h) Why should you never rack downwards with the coarse focusing knob while you are looking down the microscope? Not to break the slide.
  • 20. i) If the magnifying power of the eyepiece lens is ten times (X10), and that of the low power objective lens is four times (X4), what is the total magnification of a specimen under low power? 10 x 4 = x40
  • 21. T H E E N D