Sample Size Estimation

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Nayyar Kazmi

How to determine Sample Size in Biostatistics

Gesundheit & Medizin Business

Sample Size Estimation Nayyar Raza Kazmi

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Sample size Calculation: Objectives: Calculate sample size according to particular type of research, and purpose. Identify and select various software to calculate sample size according to particular type of research, and purpose. Why to calculate sample size? To show that under certain conditions, the hypothesis test has a good chance of showing a desired difference (if it exists) To show to the IRB committee and funding agency that the study has a reasonable chance to obtain a conclusive result To show that the necessary resources (human, monetary, time) will be minimized and well utilized. Most Important: sample size calculation is an educated guess It is more appropriate for studies involving hypothesis testing There is no magic involved; only statistical and mathematical logic and some algebra Researchers need to know something about what they are measuring and how it varies in the population of interest. SAMPLE SIZE: How many subjects are needed to assure a given probability of detecting a statistically significant effect of a given magnitude if one truly exists? POWER: If a limited pool of subjects is available, what is the likelihood of finding a statistically significant effect of a given magnitude if one truly exists? Before We Can Determine Sample Size We Need To Answer The Following: 1. What is the primary objective of the study? 2. What is the main outcome measure? Is it a continuous or dichotomous outcome? 3. How will the data be analyzed to detect a group difference? 4. How small a difference is clinically important to detect? 5. How much variability is in our target population? 6. What is the desired  and ? 7. What is the anticipated drop out and non-response % ? Where do we get this knowledge? Previous published studies Pilot studies If information is lacking, there is no good way to calculate the sample size. Type I error: Rejecting H0 when H0 is true : The type I error rate. Type II error: Failing to reject H0 when H0 is false : The type II error rate Power (1 - ): Probability of detecting group difference given the size of the effect () and the sample size of the trial (N). Estimation of Sample Size by Three ways: By using (1) Formulae (manual calculations) (2) Sample size tables or Nomogram (3) Softwares. SAMPLE SIZE FOR ADEQUATE PRECISION: In a descriptive study, Summary statistics (mean, proportion) Reliability (or) precision By giving “confidence interval” Wider the C.I – sample statistic is not reliable and it may not give an accurate estimate of the true value of the population parameter. Sample size calculation for cross sectional studies/surveys: Cross sectional studies or cross sectional survey are done to estimate a population parameter like prevalence of some disease in a community or finding the average value of some quantitative variable in a population. Sample size formula for qualitative variable and quantities variable are different.

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