The document outlines the steps in a histology procedure: 1) Specimens are accessioned and given an identifying number; 2) The specimen is described and parts are placed in cassettes during gross examination; 3) Tissue fixation preserves the specimen's structure and prevents loss of constituents; 4) Tissue processing dehydrates, clears, and impregnates the tissue to allow for embedding in wax; 5) Embedded tissue is sectioned into thin slices and stained with dyes to enhance contrast for microscopic examination.

1. HISTOLOGY PROCEDURE

3. HISTOLOGY PROCEDURE SPECIMEN ACCESSIONING TISSUE FIXATION GROSS EXAMINATION TISSUE PROCESSING DEHYDRATION CLEARING IMPREGNATION TISSUE EMBEDDING SLIDE STAINING TISSUE SECTIONING

4. HISTOLOGY PROCEDURE SPECIMEN ACCESSIONING

5. SPECIMEN ACCESSIONING Specimens are accessioned by giving them a number that will identify each specimen for each patient

6. HISTOLOGY PROCEDURE SPECIMEN ACCESSIONING GROSS EXAMINATION

7. GROSS EXAMINATION Consists of describing the specimen and placing all or parts of it into a small plastic cassette

8. HISTOLOGY PROCEDURE SPECIMEN ACCESSIONING TISSUE FIXATION GROSS EXAMINATION

9. TISSUE FIXATION The objective of fixation is to preserve cells and tissue constituents in as close a life-like state as possible

10. TISSUE FIXATION Fixation coagulates Tissue proteins and constituents, a necessary event to prevent their loss or diffusion during tissue processing

11. HISTOLOGY PROCEDURE SPECIMEN ACCESSIONING TISSUE FIXATION GROSS EXAMINATION TISSUE PROCESSING DEHYDRATION CLEARING IMPREGNATION

12. TISSUE PROCESSING The objective is to embed the tissue in a solid medium firm enough to support the tissue and give it sufficient rigidity to enable thin sections to be cut

13. TISSUE PROCESSING DEHYDRATION : Removal of fixative and water from the Tissue and replacing it with dehydrating fluid CLEARING: Replacement of dehydrating fluid with a fluid that is totally miscible with both the dehydrating fluid and the embedding medium IMPREGNATION: Replacement of clearing agent with the embedding medium

14. HISTOLOGY PROCEDURE SPECIMEN ACCESSIONING TISSUE FIXATION GROSS EXAMINATION TISSUE PROCESSING DEHYDRATION CLEARING IMPREGNATION TISSUE EMBEDDING

15. TISSUE EMBEDDING The process by which Tissues are orientated in the desired direction and are surrounded by a medium such as wax which when solidified will provide sufficient external support during sectioning

16. HISTOLOGY PROCEDURE SPECIMEN ACCESSIONING TISSUE FIXATION GROSS EXAMINATION TISSUE PROCESSING DEHYDRATION CLEARING IMPREGNATION TISSUE EMBEDDING TISSUE SECTIONING

17. TISSUE SECTIONING The process by which wax hardened tissues are cut into very thin sections, 3-5 microns, using a Microtome

18. HISTOLOGY PROCEDURE SPECIMEN ACCESSIONING TISSUE FIXATION GROSS EXAMINATION TISSUE PROCESSING DEHYDRATION CLEARING IMPREGNATION TISSUE EMBEDDING SLIDE STAINING TISSUE SECTIONING

19. SLIDE STAINING The process by which thin sections of tissue are stained using special dyes in order to enhance contrast in the microscopic image

20. HISTOLOGY PROCEDURE SPECIMEN ACCESSIONING TISSUE FIXATION GROSS EXAMINATION TISSUE PROCESSING DEHYDRATION CLEARING IMPREGNATION TISSUE EMBEDDING SLIDE STAINING TISSUE SECTIONING

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