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APPLICATION OF PHAGE TYPING AND PULSED-FIELD GEL ELECTROPHORESIS TO ANALYSE  SALMONELLA ENTERICA  ISOLATES  FROM A SUSPECTED OUTBREAK IN LAGOS, NIGERIA KABIR O. AKINYEMI  ET AL . MarĂ­a Alejandra RamĂ­rez Ruiz MarĂ­a Carolina Portela FernĂĄndez UPB - MedellĂ­n
INTRODUCTION
Salmonella
Salmonella ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Salmonella enterica
Pulsed-Field Gel Electrophoresis (PFGE)  ,[object Object],[object Object],[object Object]
Relationship in between
GENERAL OBJECTIVE
General objective ,[object Object]
MATERIALES Y MÉTODOS
Bacterias Condiciones Ăłptimas de conservaciĂłn * Las muestras eran de  S. enteritidis y/ o de  S. typhimurium.
Test de Susceptibilidad Bacteriana
Test de Susceptibilidad Bacteriana ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
FagotipificaciĂłn ,[object Object],[object Object],[object Object]
Electroforesis en gel de campo pulsado (PFGE) Ha demostrado ser altamente discriminatoria en epidemiologĂ­a
RESULTADOS
Salmonella tymphimurium
Salmonella tymphimurium ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Total: 19 muestras Similitud total: 71%
Salmonella tymphimurium RDNC: Reaccionaron pero no se conformaron DT71: Reaccionaron y se conformaron con ese fago. UT: No se tipificaron  SEROTIPO UBICACIÓN FAGOTIPIFICACIÓN PFGE CANTIDAD Salmonella typhimurium Humanos RDNC X1 2 Humanos RDNC X2 2 Agua RDNC X2 1 Humanos DT71 X3 5 Ganado DT71 X3 2 Agua DT71 X3 2 Aguas sucias DT71 X3 2 Humanos UT X4 3 TOTAL: 19
Salmonella enteritidis
Salmonella enteritidis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Total: 13 muestras Similitud total: 64%
Salmonella enteritidis RDNC: Reaccionaron pero no se conformaron PT29: Reaccionaron y se conformaron con ese fago. UT: No se tipificaron  SEROTIPO UBICACIÓN FAGOTIPIFICACIÓN PFGE CANTIDAD Salmonella enteritidis Humanos UT X1e 4 Aguas residuales UT X1e 1 Humanos RDNC X1e 3 Humanos RDNC X2e 1 Humanos RDNC X3e 1 Agua RDNC X4e 1 Ganado RDNC X4e 1 Agua PT29 X4e 1 TOTAL: 13
DISCUSSION
AUTHOR STATEMENT YES NO Laconcha I et al. PFGE and phagetyping increase the discrimination in the epidemiologic  analysis of S. enteriditis strains. X Onyango MD et al. Phagetyping profiles cathegorized as 1, 3 & 5 may be found in S. typhimurium in Africa X Ward LR et al. Phage type PT4 is the most found within S. enteritidis samples.  X Porwollik S et al. Serogroup, host specificity & disease characteristcs influence the isolates’ clustering. X
CONCLUSIONS
Conclussions ,[object Object],[object Object]
Conclussions ,[object Object],[object Object]
BIBLIOGRAFÍA
[object Object],[object Object],[object Object],[object Object],[object Object]
MAPAS CONCEPTUALES
Por: MarĂ­a Carolina Portela FernĂĄndez
Por: MarĂ­a Alejandra RamĂ­rez Ruiz

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