1. Manuela Gómez Londoño
Molecular biology
August 13 2012
UPB-Medellín

4. ZFN is an artificial protein
that binds to a short
sequence of DNA and a
DNA nuclease which cuts
at the site of binding of
ZFN, so cells cannot be
easily repaired.
Cell death is good in case
of DNA damage,
mutations, exposition to
carcinogens or radiation
and is so bad in
neurodegenerative disease
or in cells wich cant be
regenerated.

5. When ZFN gene enter
to the cell starts
producing ZFN
proteins wich destroy
specific genes of cell
DNA.

6. The bad thing is that ZFN may act on other
genes causing injury and damage to DNA, for
this they use a transport protein of ZFN

7. ZFN gene therapy is use to
interrupt the expression of
receptor CCR5 of T cells,creating
high resistance to virus.

8. This new technique with ZFN is too effective because
its actue on specific gene causing minimal damage to
DNA.

10. Toxins induce apoptosis its
start in the mitochondria,
where caspases enzymes are
activated by accumulation
of reactive oxygen species
(ROS).

11. Apoptosis may be
reversible if toxins are
deleted quickly of de
cell and depending on
the type of cell and the
time it was exposed to
the toxin and if they
are activated or not
caspases.

13. Despite of the cells can be left with damage or a
mutation because the enzymes that cleave the
AND are activated even though cell repair and
fixes the most breaks of DNA.

14. . This process is too useful for cells that do
not have the ability to regenerate as
neurons because with their death the
number reduces and will manifest different
pathologies, so in these cells is better have
some damage than to be dead because with
damages would do at least some of its
functions

15. Gene therapy has become
the future of medicine and
hope for many conditions
such as regulating gene
expression. we can take
control of life to the point
of being able to avoid most
diseases before they show
up. improving the quality
of life of people.

16. Gene therapy to make a zinc
finger nuclease solution is a
really cost effective because it has
the ability to act on specific genes
and thus ensures that the
damage, DNA mutations and
alterations is minimal. So this is a
real solution for HIV it is very
likely that you can start using in
all medical fields

17. If the cells survive this short
exposure to toxins, will be a
medical advance because it
may arrest the progression of
neurodegenerative diseases,
may also be used in therapies
against cancer, the
quimioterapia not would
have to be continuous
because it is toxic and it
would help reverse apoptosis

18. Anastasis is a big step in medicine
because we may find a cure for
cancer, something that has been
sought for decades, since we
could recover so that cells do not
regenerate and can still perform
their functions or some of them,
thus allowing a person to have a
better quality of life if you have
some disease possibly reaching
the cure.

19.  Safer alternative to experimental gene
therapy against HIV infection Published on
July 2, 2012 at 9:30 AM NEW MEDICALS
 Toxins and cell death: Interview with
Professor Denise Montell Published on July 6,
2012 at 7:09 AM NEWS MEDICALS
 MARTINEZ SÁNCHEZ, Lina María. Biología
molecular.2. ed. Medellín: UPB. Fac. de
Medicina, 2006. 208 p.