MULTIDISCIPLINRY NATURE OF THE ENVIRONMENTAL STUDIES.pptx
Tick infestation majid m. m.
1. Prevalence of tick infestation onPrevalence of tick infestation on
livestock animals in Pothwar,livestock animals in Pothwar,
PakistanPakistan
Majid MahmoodMajid Mahmood MajidMajid and Mazhar Qayyumand Mazhar Qayyum
Department of Zoology, PMAS Arid AgricultureDepartment of Zoology, PMAS Arid Agriculture
University Rawalpindi, PakistanUniversity Rawalpindi, Pakistan
2. IntroductionIntroduction
Ticks:Ticks:
• Ectoparasites: blood suckingEctoparasites: blood sucking
• Affect the productive potential of livestockAffect the productive potential of livestock
• Transmit protozoan parasites viz.,Transmit protozoan parasites viz., BabesiaBabesia spp. andspp. and
TheileriaTheileria sppspp..
3. • The information on ticks prevalence is scanty inThe information on ticks prevalence is scanty in
PakistanPakistan
• No previous study in Pothwar regionNo previous study in Pothwar region
Objective:Objective:
• To find out the prevalence of ticks infestationTo find out the prevalence of ticks infestation
pattern among different livestock animals ofpattern among different livestock animals of
Pothwar regionPothwar region
4. Materials and MethodsMaterials and Methods
Study PeriodStudy Period
• October 2008 to August 2009October 2008 to August 2009
Study AreaStudy Area
• The main study site: Barani LivestockThe main study site: Barani Livestock
Production Research Institute KherimuratProduction Research Institute Kherimurat
(BLPRI), District Attock(BLPRI), District Attock
5. • Other livestock farms surveyedOther livestock farms surveyed
– ChakwalChakwal
– Kallar KahaarKallar Kahaar
– Tala GangTala Gang
– ChakriChakri
– RawaatRawaat
– Chak Baili KhanChak Baili Khan
– Kallar SayyadanKallar Sayyadan
– National Agriculture Research Center (NARC)National Agriculture Research Center (NARC)
IslamabadIslamabad
7. TicksTicks collectioncollection
• All body parts of the animals were thoroughlyAll body parts of the animals were thoroughly
inspectedinspected
• Picked with the help of rubber coated forcepsPicked with the help of rubber coated forceps
• Stored in 70% ethyl alcoholStored in 70% ethyl alcohol
• Properly labeledProperly labeled
8. Ticks processing for identificationTicks processing for identification
• Washed with distilled waterWashed with distilled water
• Boiled in 10% KOH for 30 minutesBoiled in 10% KOH for 30 minutes
• 10% glacial acetic acid for 5 minutes10% glacial acetic acid for 5 minutes
• Washed with distilled waterWashed with distilled water
• Stained with 10% acid fuchsin for 2 minutesStained with 10% acid fuchsin for 2 minutes
• Washed with distilled waterWashed with distilled water
• Dehydrated through 30%, 50%, 70%, 80%, 90% andDehydrated through 30%, 50%, 70%, 80%, 90% and
absolute alcoholabsolute alcohol
• Cleared in clove oilCleared in clove oil
• Mounted in Canada balsam on glass slideMounted in Canada balsam on glass slide
9. IdentificationIdentification
• Morphological examination underMorphological examination under
stereomicroscopestereomicroscope
• Identified according to the keys andIdentified according to the keys and
descriptions given by Hoogstraal (1979) anddescriptions given by Hoogstraal (1979) and
WalkerWalker et alet al. (2003). (2003)
13. Tick GeneraTick Genera
• Only 700 tick specimens were identified from sheep,Only 700 tick specimens were identified from sheep,
goats and cattlegoats and cattle
• Six genera of ticks foundSix genera of ticks found
– Haemophisalis =Haemophisalis = 28.1%28.1%
– RhipicephalusRhipicephalus = 24.8%= 24.8%
– IxodeIxode = 20.0%= 20.0%
– HyalommaHyalomma = 14.1%= 14.1%
– BoophilusBoophilus = 11.5%= 11.5%
– Dermacenter =Dermacenter = 1.3%1.3%
14. • Haemophesalis:Haemophesalis: Most abundant on sheepMost abundant on sheep
• Rhipicephalis:Rhipicephalis: Most abundant on goatsMost abundant on goats
• Ixode:Ixode: Most abundant on cattleMost abundant on cattle
17. Seasonal AbundanceSeasonal Abundance
Infestation rateInfestation rate was:was:
• Very high in August and JulyVery high in August and July
• High in February, March, April, May and JuneHigh in February, March, April, May and June
• Low in October, November, December andLow in October, November, December and
JanuaryJanuary
18. ConclusionsConclusions
• Both infestation rate and average tick burden were veryBoth infestation rate and average tick burden were very
high in summer (rainy) as compared to winterhigh in summer (rainy) as compared to winter
• Both were highest in AugustBoth were highest in August
• Lower in may and June as compared to July and AugustLower in may and June as compared to July and August
• Ticks seem to prefer high humidity and temperatureTicks seem to prefer high humidity and temperature
but not dry-hot conditionsbut not dry-hot conditions