Emshwiller presentation at Smithsonian Botanical Symposium 2010, which had theme of "Food for Thought: 21st Century Perspectives on Plants and People"
Evolution & Fisheries: A Relationship?: Dr. Malin Pinsky
Human/Plant Interactions in Crop Evolution: Integrating Different Sources of Data in Contemporary Ethnobotany - Emshwiller
1. Human/Plant Interactions in Crop Evolution: Integrating Different Sources of Data in Contemporary Ethnobotany Eve Emshwiller University of Wisconsin – Madison Botany Department [email_address] 25 September 2010 Oxalis tuberosa as an example
2. oca – Oxalis tuberosa octoploid - 8 x clonal - vegetatively propagated
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6. Inka kero vessel, possibly created during early Colonial “transitional” period
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9. 2 X 8 X octoploid autopolyploid diploid progenitor
10. 8 X 2 X 2 X 2 X 2 X allopolyploid octoploid diploid progenitors
11. Origins of domestication and polyploidy of oca ITS -- internal transcribed spacer of nuclear ribosomal DNA Emshwiller and Doyle (1998) AJB 85: 975-985 ncpGS -- chloroplast-expressed, nuclear-encoded glutamine synthetase Emshwiller and Doyle (1999) MPE 12: 310-319 Emshwiller and Doyle (2002) AJB 89: 1042-1056 “ Oxalis tuberosa alliance” x = 8
12. 100 86 66 64 98 60 82 57 62 O. laxa var. hispidissima O. pachyrrhiza O. spiralis O. spiralis O. spiralis O. mollissima O. medicaginea O. lotoides O. subintegra O. unduavensi s O. oulophora O. longissim a O . sp. cfr. melilotoides O. humbertii O. lucumayensis ssp . lucumayensis O. lucumayensis ssp. subiens O. paucartambensis O. cuzcensis O. herrerae O. picchensis O. herrerae O. ptychoclada var. trichocarpa O. tabaconasensis O. peduncularis var. pilosa O. petrophila O. nubigena O. peduncularis O. weberbaueri O. sp. “V” O. flagellata O. flagellata O. andina O. ortgiesii O. boliviana O. boliviana O. vulcanicola O . sp. “C” O. sp. aff. ptychoclada O . sp. “A” 99 54 “ Oxalis tuberosa alliance” x = 8 66 87 79 99 100 100 strict consensus of 88 MP trees bootstrap values using strict consensus method Emshwiller (2002) Bot Rev 68:128-152 combined ITS and ncpGS data Cytology and name of Oxalis tuberosa alliance from: de Azkue & Martínez 1990
13. Oxalis sp. Oxalis picchensis wild tuber-bearing taxa in the Oxalis tuberosa alliance Map modified from Emshwiller (2002) Bot Rev 68:128-152
15. 8 x 2 x 2 x 2 x autoallopolyploid octoploid diploid progenitors
16. Oxalis tuberosa 2 n = 8 x = 64 Bolivian wild tuber-bearing 4 x ? O. picchensis wild tuber-bearing 4 x One hypothesis of origin of polyploidy in oca Based on ncpGS data BUT… Oxalis sp. 2 x Oxalis sp. 2 x Oxalis sp. 2 x
17. Oxalis chicligastensis Oxalis sp. Oxalis picchensis wild tuber-bearing taxa in the Oxalis tuberosa alliance Oxalis sp. Map modified from Emshwiller (2002) Bot Rev 68:128-152
18. Ethnotaxonomy of oca in three communities in the district of Pisac in southern Peru
19. P’osqo (sour cultivar used only for khaya) many wayk’u folk cultivars
20. Khaya = oca processed by soaking, freezing & drying Khaya = oca processed by soaking, freezing & drying
21. Khaya = oca processed by soaking, freezing & drying
23. 6 0 5 0 4 0 3 0 3 0 2 0 1 0 0 Frecuency Number of bands amplified (>95 kb) * * * * * * * * * P’osqo & 4x wild Oxalis Wayk’u oqa AFLP data from 1 primer combination
24. Oxalis chicligastensis Oxalis sp. Oxalis picchensis wild tuber-bearing taxa in the Oxalis tuberosa alliance Oxalis sp. Map modified from Emshwiller (2002) Bot Rev 68:128-152
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26. wild tuber-bearing taxa in the Oxalis tuberosa alliance Oxalis sp. 4x (Emshwiller 2002b) 4x? (Brücher 1969) ?? 6x? (Kelly Vivanco, in prep.) Oxalis sp. Oxalis chicligastensis Oxalis picchensis Map modified from Emshwiller (2002) Bot Rev 68:128-152
27. -0.6 -0.4 -0.2 0.0 -0.3 -0.2 -0.1 0.0 0.1 0.2 0.3 NMDS1 NMDS2 NMDS analysis of 399 AFLP markers (7 primer pairs) from cultivated oca and four wild, tuber-bearing Oxalis taxa. O. picchensis [Peru, Depts. Cusco & Apurimac] Oxalis sp. [Peru, Dept. Lima] O. chicligastensis [NW Argentina] Oxalis sp. [Bolivia] wayk’u oca khaya oca (P’osqo) 8 x 4x? Thanks to analytical help from Cécile Ané Emshwiller & al. (2009) AJB 96: 1839–1848 Kelly Vivanco, unpublished
28. Neighbor Joining analysis of AFLP data (7 primer pairs). O. picchensis wild tuber-bearing [Peru, Depts. Cusco & Apurimac] Oxalis sp. wild tuber-bearing [Peru, Dept. Lima] O. chicligastensis wild tuber-bearing [NW Argentina] Oxalis sp. wild tuber-bearing [Bolivia] “ Wayk’u oqa” – cultivated oca used either for cooking after sunning (or for khaya) “ P’osqo oqa” – sour ocas used only for khaya Emshwiller & al. (2009) AJB 96: 1839–1848
29. trnT-trnL 1 of 5 MPTs O ortgiesii ORT O boliviana EE921 O rigidicaulis boliviana AAV5415 O yungasensis EE1079 O virgata EE292 O cuzcensis EE973 O cuzcensis EE982 O cuzcensis EE1123 O cuzcensis EE606 O nubigena EE188 O nubigena EE654 O nubigena EE701 O spiralis Comas EE590 O sp nov Comas EE592 O phaeotricha EE497 O teneriensis EE605 O ptychoclada EE916 O urubambensis EE926 O sp EE979 O sp EE971 O cuzcensis EE887 O picchensis EE500 O picchensis EE1152 O chicligastensis EE1001c O chicligastensis EE1002 O chicligastensis EE1003 O chicligastensis A O chicligastensis B O sp w tubers Bolivia EE284 O sp w tubers Dept Lima EE1110 O sp w tubers Dept Lima EE1169 O sp w tubers Dept Lima EE1185 O sp w tubers Bolivia EE259 O sp w tubers Bolivia EE260 O sp w tubers Bolivia EE260C O sp w tubers Bolivia EE262 O tuberosa 50 02 O tuberosa 46 14 O tuberosa 40 02 O tuberosa 19 01 O tuberosa 11 07 O. picchensis [Peru, Depts. Cusco & Apurimac] O. sp. wild tuber-bearing [Peru, Dept. Lima] O. sp. wild tuber-bearing [Bolivia] O. tuberosa cultivated oca, both wayk’u and khaya [Peru, Dept. Cusco] O. chicligastensis [nw Argentina] [Bolivia] Oxalis tuberosa alliance Still to be confirmed… Preliminary data. Please do not cite!
30. wild tuber-bearing taxa in the Oxalis tuberosa alliance Oxalis sp. 4x (Emshwiller 2002b) 4x? (Brücher 1969) ?? 6x? (Kelly Vivanco, in prep.) Oxalis sp. Oxalis chicligastensis Oxalis picchensis Map modified from Emshwiller (2002) Bot Rev 68:128-152
31. wild tuber-bearing taxa in the Oxalis tuberosa alliance Map modified from Emshwiller (2002) Bot Rev 68:128-152 Guitarrero Cave is outside of the (present day) distributions of the likely progenitors of cultivated oca.
35. KHAYA WAYK'U p'osqo kusipata ushpa panti q'ellu panti yuraq panti machasqa puka p'osqo misitu hank'o q'ello q'ellu k'aytu yuraq kishwar Damaso puka chaleco chachapiya waqankillay use categories folk cultivar names sub-cultivars yana misitu tullu misitu higos misitu k'aspi misitu q'ellu misitu yana ushpa puka ushpa yuraq ushpa for processing by freezing and drying for cooking fresh after sun-sweetening Emshwiller, unpublished data (field notes from 1997)
36. Panti Hanq’o Q’ello Kusipata Yana Ushpa Puka Ushpa Yuraq Kishwar Misitu Q’ellu Panti P’osqo Q’ellu K’aytu Machasqa Emshwiller, unpublished data (field notes from 1997)
37. Sn qKy Sn Sn Oxalis chicligastensis Oxalis sp. (wild tuber-bearing of Bolivia) Oxalis picchensis 0.005 changes NJ Puka Chiliku (chaleco) Ushpa Yuraq Kishwar Misitu / Higos Puka P’osqo Machasqa Damaso “ yellow group” : Q’ellu K’aytu Hanq’o Q’ello / Waqankillay Q’ellu Panti / Señorita Puka Panti Kusipata P’osqo M M M M Yana Ushpa ( & Yana Kishwar) Puka Ushpa Yuraq Ushpa Yana Misitu Q’ellu Misitu Higos Misitu Tullu Misitu K’aspi Misitu { { * * * * * * * * * Señorita P’osqo Ushpa Kishwar Kusipata Se ñ orita Puka Panti Puka Chiliku Misitu Hanq’o Q’ellu Puka P’osqo Machasqa Damaso Q’ellu K’aytu AFLP data from 1 primer combination * * { Emshwiller (2006) Chapter in Darwin’s Harvest (Motley & al. eds.)
38. Somatic mutations lead to chimeric tubers, and then to clone-mates of differing pigmentation?
39. K. S. Zimmerer 2003 Soc. & Nat. Res. 16: 583-601 Seed Network (“seed flow”) studies via surveys of farmers &/or market vendors
40. ? ? ? ? ? ? gifts theft sharecropping payment-in-kind barter cash sale relatives and compadres within same community within district other communities Pisac Market ? ? Different modes of exchange operate at different scales Emshwiller, unpublished data (field notes from 1997)
42. Cusco Puno Cochabamba La Paz “ Ancient trade routes” hypothesis based on CIRNMA’s market surveys image source: USGS DEM and the Cornell Andes Project
44. Are patterns of distribution of clonal genotypes of oca correlated with distributions of different human languages or dialects? Quechua dialect map A. Chirinos Rivera (2001) Atlas Lingüístico del Perú
45. – Eve Emshwiller R. Carolina Girón A. Victor Nina M. Juan Tineo C. J. Eduardo Angeles M. Policarpo Catacora C. Tulio Medina H. Field Expeditions led by Coordinators of Genetic Resources at 5 of INIA’s experiment stations
46. and Kelly Vivanco, Nery Amaru C., Erwin Peralta, Carlos Antonio Hernandez, Rufino Martínez Cucho, Salome Altamirano Y., Manuela Huacachi Q., Alfredo Tupayachi H., Maibé Olivera G., Manuel Concha Menacha, Jorge Luís Millones I., Javier Ríos V., Roque Alberco, Eulalia Vargas, Isaias Achata T., Julio Huaripata C., Wilbert Cruz H., Iramí Pérez Hurtado
66. Moran’s I for oca clones (that have over 5 representatives in sample) These preliminary results will be examined more closely soon. Stay tuned… Preliminary data. Please do not cite!