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WineLab: fast, simple and
reliable wine testing system
         Doc. Simone Bellassai
             R & D Chemist
      s.bellassai@cdr-mediared.it
CDR s.r.l.


CDR, development and engineering company

The fields of application of CDR systems are:

Telematics: automatic tools for motorway stations and parking
   places
Medical diagnostics: hematology, hemostasis and veterinary
   systems
Food diagnostics: instruments and reagents for rapid,
   accurate and safe food analysis
Wine diagnostics
                       WineLab




Grapes analysis                       Must analysis




                      Wine analysis
Wine diagnostics

                             WineLab
        Antocyanes                                Polyphenols


      Y.A.N.                                        Free SO2


 Total acidity                                       Total SO2

                                                       Alcohol Content
Glucose and Fructose
             pH                                    Lactic Acid

                  Volatile acidity   Malic Acid
System overview

   WineLab system is constituted by:

• A thermostated analyzer based on photometric
  technology which uses solid-state emitters
• Kits with pre-filled cuvettes and reagents, developed by
  our research laboratory, ready to use
• Pipettes
• Print Manager, a software which enables to connect
  the analyzer to the PC
• User's manual
• Illustrated data sheets
• 3 years warranty
Features of the analyzer

•   System: 4 wavelenghts and 12
    incubation cells designed to
    heat samples at a temperature
    of 37°C
•   Measurement: based on the
    reading of absorbance
•   Photometric range: 0.0-2.3
    Abs; (0.0-4.0 Abs) ampl.
•   Hardware and Software:
    designed and manufactured by
    CDR S.r.l.
•   Maintenance: not necessary
    periodic calibration
Tests
Tests
Competitors matrix


                                             External    Traditional
                  WineLab          NIR
                                            Laboratory    Systems
  Simplicity        High          High          -         Very Low

Analyzer price      Low         Very High       -            -

Analysis price    Medium          Low        Medium      Medium

Analysis speed     Medium         High        Low           Low

  Calibration    Not required   Required        -         Required


      Very Important!!
ISVEA validation


●   CdR – WineLab system is validated by ISVEA



●   This system doesn't need calibration. It is already
    calibrated and well correlated to the official methods
ISVEA validation

                                        Acido L-Malico su vino
                                             Malic Acid                                                                           Volatile acidity volatile) su vino
                                                                                                                                   Acido Acetico (acidità
                                                                                                                                                          (acetic acid)
                         2                                                                                              1,4
                        1,8
                                        2                                                                               1,2
                        1,6            R = 0,9837
                                   2
                                                                                                                                  R2R = 0,9709




                                                                                                OIV (g/l ac. Acetico)
                                  R =0,98                                                                                           =0,97
                                                                                                                                           2
HPLC (g/l ac. Malico)




                        1,4                                                                                              1
                        1,2
                                                                                                                        0,8
                         1
                        0,8                                                                                             0,6
                        0,6
                                                                                                                        0,4
                        0,4
                        0,2                                                                                             0,2

                         0
                                                                                                                         0
                              0        0,5              1               1,5    2
                                                                                                                              0      0,2       0,4     0,6      0,8      1   1,2   1,4
                                             WineLab (g/l ac. Malico)
                                                                                                                                                 WineLab (g/l ac. Acetico)




                                                                                       Sugars

                                                                                   R2=0,97
Glucose and fructose in wine

                                                   Fructose in wine
                                                Fructose in wine                                                                            Glucoseinin wine
                                                                                                                                               Glucose wine

                            7                                                                                             3,5


                            6                                                                                              3



                                      R2= 0,9755
                                         =0,97                                                                                        R2=0,97
                                                                                                                                            2
                            5          R
                                            2
                                                                                                                          2,5          R = 0,9704




                                                                                                     HPLC (g/l glucose)
      HPLC (g/l fructose)




                            4                                                                                              2


                            3                                                                                             1,5


                            2                                                                                              1


                            1                                                                                             0,5


                            0                                                                                              0
                                0,0   1,0        2,0        3,0      4,0        5,0    6,0   7,0                                0,0   0,5       1,0       1,5       2,0       2,5   3,0   3,5

                                                       WineLab (g/l fructose)                                                                         WineLab (g/l glucose)




                                                                                      HK + G6P-DH
Glucose + ATP + NAD+                                                                                                                  ADP + gluconate-6-P + NADH


                                                                                 HK + G6P-DH + PGI
Fructose + ATP + NAD                                         +
                                                                                                                                      ADP + gluconate-6-P + NADH
Glucose and fructose in wine

                       CdR-WineLab's advantages

●   Determination of assimilable sugars! Pentose sugars are excluded


●   You have a detection limit of 0.1 g/L! You can know with high
    precision, in the alcohol fermentation, when the sugars are finished,
    to avoid any risk of acetic acid.


●
    You can do up to 30 analysis/hour.
Free sulfur dioxide

Step 1: taking and incubation of the sample
Free sulfur dioxide

Step 2: addition of the R2 and reading of the sample
Yeast Assimilable Nitrogen


                     Inorganic Nitrogen(ammonium)
           HO                                      HO
                 O                                        O
       O                 H                 Gl-DH H N
                           +                      2                     +
                         N       NADH                             NAD
                       H    H
                         H
                 O                                        O
           HO         Ammonium
                                                    HO
  keto-glutarate                              Glutammate

                     Organic nitrogen (α-aminic)
                                                          R

                           O         OH
                                                              S

      R     O                                                      R
                                 O         SH                 N
    H 2N    OH                   O    NH                               O
                           O                                      HO
                                     CH3

    Amino acid          OPA      NAC               isoindolic derivative
Yeast Assimilable Nitrogen


                     CdR-WineLab's advantages
●   You can check separately the amount of inorganic nitrogen and
    organic nitrogen.


●
    The test is not affected by proline and hidroxy-proline
    interference.


●
    You can do up to 30 tests/hour.
Users

Thanks to its simplicity and reliability, WineLab can be used by:

         User                            Advantages
                           •carry out independently the most
                           important analysis on wine
  Small and medium
                           •affordable analyzer price
      wineries
                           •lower test price compared to
                           laboratories
                           •carry out analysis on a small samples
                           quantity without using expensive and
     Big wineries
                           complicated automatic analysis
                           systems
                           •check the most important parameters
Enological consultants     during wine production in the wineries
                           •create a “portable laboratory”
The NEXT NEW TEST


              TOTAL POLYPHENOL INDEX
●   It is available by the beginning of june


●   The polyphenol index is a reading of absorbance at 280nm. So you
    can do this without using an UV lamp and without quarz cuvettes


●   You can use this test in the mixture of wines to control and keep the
    right polyphenols level
WineColor


• WineColor is a complementar analyzer to do Colour
  Intensity and Tonality reading the absorbance at 420,
  520, and 620 nm

• You can do these analysis without dilution of the
  sample

• You can control and correct the colour of the wine
Summarizing....


●   WineLab is a rapid simple and reliable system. This analyzer is able
    to control each step of the vinification from grapes to wine in bottle


●   The winery is autonomous for the analysis and you can have
    a result in real time without waiting for the result from external
    laboratory
●   WineLab gives an enological consultant the opportunity to
    work without opening a laboratory
CdR- WineLab



      Thanks for
     your attention




   Doc. Simone Bellassai
       R & D Chemist
s.bellassai@cdr-mediared.it

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Wine Test Equipment - WineLab

  • 1. WineLab: fast, simple and reliable wine testing system Doc. Simone Bellassai R & D Chemist s.bellassai@cdr-mediared.it
  • 2. CDR s.r.l. CDR, development and engineering company The fields of application of CDR systems are: Telematics: automatic tools for motorway stations and parking places Medical diagnostics: hematology, hemostasis and veterinary systems Food diagnostics: instruments and reagents for rapid, accurate and safe food analysis
  • 3. Wine diagnostics WineLab Grapes analysis Must analysis Wine analysis
  • 4. Wine diagnostics WineLab Antocyanes Polyphenols Y.A.N. Free SO2 Total acidity Total SO2 Alcohol Content Glucose and Fructose pH Lactic Acid Volatile acidity Malic Acid
  • 5. System overview WineLab system is constituted by: • A thermostated analyzer based on photometric technology which uses solid-state emitters • Kits with pre-filled cuvettes and reagents, developed by our research laboratory, ready to use • Pipettes • Print Manager, a software which enables to connect the analyzer to the PC • User's manual • Illustrated data sheets • 3 years warranty
  • 6. Features of the analyzer • System: 4 wavelenghts and 12 incubation cells designed to heat samples at a temperature of 37°C • Measurement: based on the reading of absorbance • Photometric range: 0.0-2.3 Abs; (0.0-4.0 Abs) ampl. • Hardware and Software: designed and manufactured by CDR S.r.l. • Maintenance: not necessary periodic calibration
  • 9. Competitors matrix External Traditional WineLab NIR Laboratory Systems Simplicity High High - Very Low Analyzer price Low Very High - - Analysis price Medium Low Medium Medium Analysis speed Medium High Low Low Calibration Not required Required - Required Very Important!!
  • 10. ISVEA validation ● CdR – WineLab system is validated by ISVEA ● This system doesn't need calibration. It is already calibrated and well correlated to the official methods
  • 11. ISVEA validation Acido L-Malico su vino Malic Acid Volatile acidity volatile) su vino Acido Acetico (acidità (acetic acid) 2 1,4 1,8 2 1,2 1,6 R = 0,9837 2 R2R = 0,9709 OIV (g/l ac. Acetico) R =0,98 =0,97 2 HPLC (g/l ac. Malico) 1,4 1 1,2 0,8 1 0,8 0,6 0,6 0,4 0,4 0,2 0,2 0 0 0 0,5 1 1,5 2 0 0,2 0,4 0,6 0,8 1 1,2 1,4 WineLab (g/l ac. Malico) WineLab (g/l ac. Acetico) Sugars R2=0,97
  • 12. Glucose and fructose in wine Fructose in wine Fructose in wine Glucoseinin wine Glucose wine 7 3,5 6 3 R2= 0,9755 =0,97 R2=0,97 2 5 R 2 2,5 R = 0,9704 HPLC (g/l glucose) HPLC (g/l fructose) 4 2 3 1,5 2 1 1 0,5 0 0 0,0 1,0 2,0 3,0 4,0 5,0 6,0 7,0 0,0 0,5 1,0 1,5 2,0 2,5 3,0 3,5 WineLab (g/l fructose) WineLab (g/l glucose) HK + G6P-DH Glucose + ATP + NAD+ ADP + gluconate-6-P + NADH HK + G6P-DH + PGI Fructose + ATP + NAD + ADP + gluconate-6-P + NADH
  • 13. Glucose and fructose in wine CdR-WineLab's advantages ● Determination of assimilable sugars! Pentose sugars are excluded ● You have a detection limit of 0.1 g/L! You can know with high precision, in the alcohol fermentation, when the sugars are finished, to avoid any risk of acetic acid. ● You can do up to 30 analysis/hour.
  • 14. Free sulfur dioxide Step 1: taking and incubation of the sample
  • 15. Free sulfur dioxide Step 2: addition of the R2 and reading of the sample
  • 16. Yeast Assimilable Nitrogen Inorganic Nitrogen(ammonium) HO HO O O O H Gl-DH H N + 2 + N NADH NAD H H H O O HO Ammonium HO   keto-glutarate Glutammate Organic nitrogen (α-aminic) R O OH S R O R O SH N H 2N OH O NH O O HO CH3 Amino acid OPA NAC isoindolic derivative
  • 17. Yeast Assimilable Nitrogen CdR-WineLab's advantages ● You can check separately the amount of inorganic nitrogen and organic nitrogen. ● The test is not affected by proline and hidroxy-proline interference. ● You can do up to 30 tests/hour.
  • 18. Users Thanks to its simplicity and reliability, WineLab can be used by: User Advantages •carry out independently the most important analysis on wine Small and medium •affordable analyzer price wineries •lower test price compared to laboratories •carry out analysis on a small samples quantity without using expensive and Big wineries complicated automatic analysis systems •check the most important parameters Enological consultants during wine production in the wineries •create a “portable laboratory”
  • 19. The NEXT NEW TEST TOTAL POLYPHENOL INDEX ● It is available by the beginning of june ● The polyphenol index is a reading of absorbance at 280nm. So you can do this without using an UV lamp and without quarz cuvettes ● You can use this test in the mixture of wines to control and keep the right polyphenols level
  • 20. WineColor • WineColor is a complementar analyzer to do Colour Intensity and Tonality reading the absorbance at 420, 520, and 620 nm • You can do these analysis without dilution of the sample • You can control and correct the colour of the wine
  • 21. Summarizing.... ● WineLab is a rapid simple and reliable system. This analyzer is able to control each step of the vinification from grapes to wine in bottle ● The winery is autonomous for the analysis and you can have a result in real time without waiting for the result from external laboratory ● WineLab gives an enological consultant the opportunity to work without opening a laboratory
  • 22. CdR- WineLab Thanks for your attention Doc. Simone Bellassai R & D Chemist s.bellassai@cdr-mediared.it