an explanation of the barcoding pipeline, what data objects need to be tracked through the pipeline, and the possible entry and exit points to the pipeline

1. The Barcoding Pipeline Amy Driskell Laboratories of Analytical Biology (LAB) National Museum of Natural History Smithsonian Institution

2. Module II Module III

3. A Simplified Version Specimen Acquisition Extraction & Data Generation Data Mgmt Barcode Sequence Deposition

4. A Simplified Version Data Mgmt Specimen Acquisition Extraction & Data Generation Barcode Sequence Deposition Locality Permit Info Age, sex, Collector Name Photos Identifier Taxonomy Museum ID Lab processes Taxon specific info Extraction Method Primers PCR protocols extract location Cleaned barcode sequence Trace Files Assembly parameters Raw data processing

5. All data downstream from specimen collection/harvesting event must maintain their connection to the specimen!!

8. Pipeline Use User A: handles all steps from start to finish Specimen Acquisition Extraction & Data Generation Data Mgmt Barcode Sequence Deposition Data User

9. Pipeline Use User B: hands off specimens for data generation Specimen Acquisition Extraction & Data Generation Data Mgmt Barcode Sequence Deposition Data User

10. Pipeline Use User C: hands off specimens, uses barcode data Specimen Acquisition Extraction & Data Generation Data Mgmt Barcode Sequence Deposition

11. Whatever your role in the barcoding pipeline: Start out right  thoroughly and correctly collect and store all metadata at the time of specimen collection and TREAT IT AS WELL AS YOU DO THE SPECIMEN All other aspects of barcoding flow downstream from here.