1. The Analysis of the HIV Co-
Receptor Gene from 50
Individuals from Northeastern
Ohio
By: Connor Anderson, Alex Fulton, Jacqueline Makowski,
Alexis Shawver, Megan Sheldon and Victoria Soewarna
Supervisors: Dr. Harry Kestler

2.  Deletion of 32 base pairs (Δ32)
 Co-receptor of CCR5 is absent from the cell
 Less susceptible to HIV through mucosal
transfer
Delta- Δ32/ Δ32

3.  More prevalent in Caucasians
◦ 20% are carriers Δ32/w+t
◦ 1% have the deletion Δ32/Δ32
 Frequency of Δ32 mutation in other races
is unknown
 We have examined the frequency of
CCR5 Δ32 in The Early College
population.
Race!

4.  Students and teachers at Early College High
School
◦ Freshman, Sophomores, and Staff
 Approximately fifty participants
 Used samples retrieved from the inside of
ones cheek and determined if that individual
had the deletion mutation
Who?

5. ◦ Participants were given a sterile swab
 Swabbed the inside of cheek for one
minute
 Placed swab in a tube containing a lysing
agent and extracted the DNA.
 Tubes were randomly labeled.
 PCR was performed on the samples
 PCR samples were analyzed by agarose
gel electrophoresis
Protocol…

7. PCR to Detect Δ32

8.  Each individuals CCR5 status is
unknown
 The percentage of students and teachers
at Early College High School with the
deletion was determined
 10%were found to be heterozygous for
the Δ32 deletion mutation

9. Thank You!

10. N
Original Article
Brief Report
Long-Term Control of HIV by CCR5 Delta32/Delta32 Stem-Cell Transplantation
GeroHütter, M.D., Det.al. N Engl J Med 2009; 360:692-698 February 12, 2009