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C Partridge
(Team Avocado/Avo Systems Ltd)
• Soil applied Ridomil, Aliette foliar sprays and
  trunk painting were early Pc control measures.

• Injection technology initiated in early 1980’s.

• Injection has since become a method of
  controlling Pc worldwide.
• Using low pressure syringes, a buffered
  phosphonate solution is injected into the
  trunks of avocado trees.
• Uptake time can vary considerably.
• Phosphonate moves up the tree in the xylem
  and then down to the root system via the
  phloem.
• Phosphonate accumulated in the root system
  protects against invasion by Pc.
• Understanding the phenological growth cycles
  of the tree – ‘sink strength’ of different plant
  parts varies over time.
• Establishing critical root phosphonate
  concentrations required to attain Pc control
  (20-30ppm).
• Development of a commercial plant tissue
  phosphonate analysis service.

(Darvas, Pegg, Whiley, Thomas et al)
• Phosphonate applied aerially to Jarrah forests
  to control Pc in W Australia.
• Found that such applications severely reduced
  pollen viability of Jarrah.
• Trial done on avocados, found that in vitro,
  phosphonate concentrations of 2000ppm+,
  severely reduced avocado pollen germination.
• Foliar applications of phosphonate to avocados
  should be avoided at flowering, especially if
  conditions for fruitset are known to be less
  than ideal.
• Provided this is done, no negative affects
  should be found.
• We don’t foliar spray in NZ - it is being found
  to be less effective than injecting in Australia
  as well (Thomas et al)……….
  we INJECT our trees.
• We often have conditions less than favourable
  for fruitset.
• For very sick trees, one could argue that
  almost any time is a good time to inject.
• Injection timing recommendations vary from
  country to country (different phenological
  cycling).
• For NZ – ‘best administered during summer
  between early spring (July/Aug) and a
  secondary injection in the New Year period’.
  ‘Where actively growing shoots are present,
  phosphonate remains fixed in leaf tissue and
  does not reach the roots’.
(Cutting & AIC Growers Manual)
• For ‘healthy’ trees, I think not, because:-
  1. July/Aug/Sep is a time when expanding
  flower buds are the strongest sink.
  2. If phosphonate can negatively affect pollen
  viability, that would make early spring an even
  worse time to inject.
  3. Commercial root phosphonate monitoring
  gave indications that July/Aug treatment often
  gives poor phosphonate persistence in the
  roots.
4. Feb/Mar treatment however, appears to
give persistence for up to 11 months in many
cases.

This makes sense, since shoot flushes are
more mature and roots are becoming a
stronger sink at this time.
Grower   Injected   Analysed ppm
A        Sep 01     Sep 02   16
B        Sep 02     Jan 03   12
C        Aug 02     Jan 03   26
D        Feb 02     Aug 02   34
D        Feb 02     Jan 03   21
E        Mar 02     Jan 03   90
• Two sites (Lankshear & Cotterell) were chosen
• Treatments at both sites as follows:-
  (all sites – 5 trees per treatment)
  -Inject in Aug 03 with 15% HiPK
  -Control (no injection)
• Phosphonate concentrations in the roots prior
  to injecting.

• Phosphonate concentrations in the roots and
  developing flower buds at various times after
  treatment.
Cotterell roots
                        250



                        200
Concentration (mg/kg)




                                                                    Treated
                        150                                         Control


                        100



                        50



                         0
                              18-Aug   8-Sep         2-Oct       3-Dec        5-Feb
                                                 Sample date
Lankshear roots
                         250



                         200
Concenetration (mg/kg)




                                                                      control
                                                                      treated
                         150



                         100



                         50



                          0
                               18-Aug   8-Sep      2-Oct      3-Dec         5-Feb
                                                Sample date
Cotterell Flowers
                        250



                        200

                                                                     Treated
Concentration (mg/kg)




                                                                     Control
                        150



                        100



                        50



                         0
                              18-Aug   8-Sep      2-Oct      3-Dec    5-Feb
                                               Sample Date
Lankshear Flowers
                        250


                                                                     Treated
                        200
                                                                     Control
concentration (mg/kg)




                        150



                        100



                        50



                         0
                              18-Aug   8-Sep      2-Oct      3-Dec      5-Feb
                                               sample date
• Root phosphonate concentrations for Aug
  injected trees, dropped to below the threshold
  of 30ppm within 5 months at Lankshear.
• Flower phosphonate levels peaked rapidly,
  reaching 200ppm – flower tissue is a strong
  ‘sink’.
• There is a question mark about a 200ppm
  effect on pollen viability under field conditions.
• Would the concentration be more dilute in
  large trees?
• From commercial results for healthy trees,
  injecting in Feb/Mar appears to be a ‘better’
  time to inject and would be my
  recommendation if injecting once per year in
  New Zealand.
• Feb/Mar is a more ‘comfortable’ time to inject.
• In Feb/Mar, uptake is much faster.
• For very large trees, 15% sometimes still
  struggles to achieve a root level above 30ppm
  – consider using 20% on large trees if injecting
  once per year.
• Aongatete Coolstores for financial
  assistance.
• Aongatete field staff for doing the injecting.
• J & C Cotterrell & J Lankshear for use of
  their trees.
• H Pak for trial design & data analysis.
• T Whiley, G Thomas & S Ogle for helpful
  discussion.

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Colin Partridge

  • 2. • Soil applied Ridomil, Aliette foliar sprays and trunk painting were early Pc control measures. • Injection technology initiated in early 1980’s. • Injection has since become a method of controlling Pc worldwide.
  • 3. • Using low pressure syringes, a buffered phosphonate solution is injected into the trunks of avocado trees. • Uptake time can vary considerably. • Phosphonate moves up the tree in the xylem and then down to the root system via the phloem. • Phosphonate accumulated in the root system protects against invasion by Pc.
  • 4.
  • 5. • Understanding the phenological growth cycles of the tree – ‘sink strength’ of different plant parts varies over time. • Establishing critical root phosphonate concentrations required to attain Pc control (20-30ppm). • Development of a commercial plant tissue phosphonate analysis service. (Darvas, Pegg, Whiley, Thomas et al)
  • 6.
  • 7. • Phosphonate applied aerially to Jarrah forests to control Pc in W Australia. • Found that such applications severely reduced pollen viability of Jarrah. • Trial done on avocados, found that in vitro, phosphonate concentrations of 2000ppm+, severely reduced avocado pollen germination.
  • 8. • Foliar applications of phosphonate to avocados should be avoided at flowering, especially if conditions for fruitset are known to be less than ideal. • Provided this is done, no negative affects should be found.
  • 9. • We don’t foliar spray in NZ - it is being found to be less effective than injecting in Australia as well (Thomas et al)………. we INJECT our trees. • We often have conditions less than favourable for fruitset.
  • 10. • For very sick trees, one could argue that almost any time is a good time to inject. • Injection timing recommendations vary from country to country (different phenological cycling). • For NZ – ‘best administered during summer between early spring (July/Aug) and a secondary injection in the New Year period’. ‘Where actively growing shoots are present, phosphonate remains fixed in leaf tissue and does not reach the roots’. (Cutting & AIC Growers Manual)
  • 11. • For ‘healthy’ trees, I think not, because:- 1. July/Aug/Sep is a time when expanding flower buds are the strongest sink. 2. If phosphonate can negatively affect pollen viability, that would make early spring an even worse time to inject. 3. Commercial root phosphonate monitoring gave indications that July/Aug treatment often gives poor phosphonate persistence in the roots.
  • 12. 4. Feb/Mar treatment however, appears to give persistence for up to 11 months in many cases. This makes sense, since shoot flushes are more mature and roots are becoming a stronger sink at this time.
  • 13. Grower Injected Analysed ppm A Sep 01 Sep 02 16 B Sep 02 Jan 03 12 C Aug 02 Jan 03 26 D Feb 02 Aug 02 34 D Feb 02 Jan 03 21 E Mar 02 Jan 03 90
  • 14. • Two sites (Lankshear & Cotterell) were chosen • Treatments at both sites as follows:- (all sites – 5 trees per treatment) -Inject in Aug 03 with 15% HiPK -Control (no injection)
  • 15. • Phosphonate concentrations in the roots prior to injecting. • Phosphonate concentrations in the roots and developing flower buds at various times after treatment.
  • 16.
  • 17. Cotterell roots 250 200 Concentration (mg/kg) Treated 150 Control 100 50 0 18-Aug 8-Sep 2-Oct 3-Dec 5-Feb Sample date
  • 18. Lankshear roots 250 200 Concenetration (mg/kg) control treated 150 100 50 0 18-Aug 8-Sep 2-Oct 3-Dec 5-Feb Sample date
  • 19. Cotterell Flowers 250 200 Treated Concentration (mg/kg) Control 150 100 50 0 18-Aug 8-Sep 2-Oct 3-Dec 5-Feb Sample Date
  • 20. Lankshear Flowers 250 Treated 200 Control concentration (mg/kg) 150 100 50 0 18-Aug 8-Sep 2-Oct 3-Dec 5-Feb sample date
  • 21. • Root phosphonate concentrations for Aug injected trees, dropped to below the threshold of 30ppm within 5 months at Lankshear. • Flower phosphonate levels peaked rapidly, reaching 200ppm – flower tissue is a strong ‘sink’.
  • 22. • There is a question mark about a 200ppm effect on pollen viability under field conditions. • Would the concentration be more dilute in large trees? • From commercial results for healthy trees, injecting in Feb/Mar appears to be a ‘better’ time to inject and would be my recommendation if injecting once per year in New Zealand.
  • 23. • Feb/Mar is a more ‘comfortable’ time to inject. • In Feb/Mar, uptake is much faster. • For very large trees, 15% sometimes still struggles to achieve a root level above 30ppm – consider using 20% on large trees if injecting once per year.
  • 24. • Aongatete Coolstores for financial assistance. • Aongatete field staff for doing the injecting. • J & C Cotterrell & J Lankshear for use of their trees. • H Pak for trial design & data analysis. • T Whiley, G Thomas & S Ogle for helpful discussion.