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ORAL MICROBIOLOGY
Normal Microbial Flora

   Normal oral cavity contains a mixture of organisms which
    include bacteria, fungi, protozoa and occasionally virus.
   Some of the organisms are almost always in normal oral
    cavity and are called Resident Flora.
   Some organisms stay for a limited period in the oral cavity
    of a few individuals. These are named as Transient Flora.
Microbial flora of mouth

   Among all micro-organism present in the mouth
    bacteria are most predominant.

   These includes both aerobic and anaerobic
    bacteria
cocci
GRAM POSITIVE COCCI
 Strep.mutans
 Strep.sanguis
 Strep.salivarius
 Strep.mitior
 Strep.milleri
 Enterococcus sps
 Peptostreptococcus aps
 Micrococcus sps
 Staphylococcus sps
GRAM NEGATIVE COCCI
 Veillonella sps

 Neisseria sps

 Branhamella sps
Bacilli
GRAM POSITIVE BACILLI
 Lactobacillus sps
 Actinomycetes sps
 Propionibacterium sps
 Arachnia sps
 Bifidobacterium sps
 Bacterionema sps
 Eubacterium sps
GRAM NEGATIVE BACILLI
 Actinobacillus sps
 Fusobacterium sps
 Bacteroids sps
 Capanocytophaga sps
 Leptotrichina sps
 Treponema sps
 Haemophilus sps
 Selenomonas sps
 Eikenella sps
 Wollinella sps
   Of all bacteria present in mouth, streptococci are
    predominant group.
   Various species of Streptococci are encountered in mouth.
   Most of these are alpha-haemolytic
    streptococci, collectively named as viridians streptococci
Lactobacilli are frequently found in mouth.

They are imp bacteria to promote the progress of
carious lesion after the initiaton of dental caries
by Strep mutans
 Veilonella is the most common among Gram-
  negative bacteria.
 Fusobacterium nucleatum is the most commonly
  occurring Gram-negative anaerobic bacillus
 Other anaerobes such as Bacteroids,
  Leptotrichia, Selenomonas etc have also been
  found to be an imp group
   Oral treponema(T.vincenti,T.denticola,T.orale) are
    comman inhabitants of gingival crevice & sub
    gingival area
   Due to their presence in subgingival plagues, they
    have been imp role in causing gingivitis and most
    forms of periodontitis.
   Besides bacteria normal flora of mouth may contain fungi
    and protozoa
   FUNGI
   Candida albicans
   C.tropicalis
   C.pseudotropicalis
   C.stellatoidea
   Cryptococcus sps
   PROTOZOA
   Entamoeba gingivalis
   Trichomanas tenax
Acquisition Of Normal Flora Of Mouth

   The mouth of newborn at birth is usually
    microbiologically sterile.

   Within few hours of birth Streptococci establish
    themselves in the mouth.
Acquisition Of Normal Flora Of Mouth – Cont’d

   Among Streptococci, Strep. salivarius is dominant
    at this stage.

   During the first year of life other species of
    Streptococci,Staphylococci,Neisseriae,Veilonella,
    Actinomyces,Lactobacilli and Fusobacteria also
    colonize.
Acquisition Of Normal Flora Of Mouth – Cont’d

   With the eruption of teeth, solid surfaces in the
    oral cavity are available for colonization.
   Strep mutans, Strep sanguis establish themselves
    on these hard surfaces.

   Simultaneously, there is also increase in number
    of obligate anaerobes
Thank you

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A Class on Oral microbiology by Aruni puthuppally

  • 2. Normal Microbial Flora  Normal oral cavity contains a mixture of organisms which include bacteria, fungi, protozoa and occasionally virus.  Some of the organisms are almost always in normal oral cavity and are called Resident Flora.  Some organisms stay for a limited period in the oral cavity of a few individuals. These are named as Transient Flora.
  • 3. Microbial flora of mouth  Among all micro-organism present in the mouth bacteria are most predominant.  These includes both aerobic and anaerobic bacteria
  • 4. cocci GRAM POSITIVE COCCI  Strep.mutans  Strep.sanguis  Strep.salivarius  Strep.mitior  Strep.milleri  Enterococcus sps  Peptostreptococcus aps  Micrococcus sps  Staphylococcus sps
  • 5. GRAM NEGATIVE COCCI  Veillonella sps  Neisseria sps  Branhamella sps
  • 6. Bacilli GRAM POSITIVE BACILLI  Lactobacillus sps  Actinomycetes sps  Propionibacterium sps  Arachnia sps  Bifidobacterium sps  Bacterionema sps  Eubacterium sps
  • 7. GRAM NEGATIVE BACILLI  Actinobacillus sps  Fusobacterium sps  Bacteroids sps  Capanocytophaga sps  Leptotrichina sps  Treponema sps  Haemophilus sps  Selenomonas sps  Eikenella sps  Wollinella sps
  • 8. Of all bacteria present in mouth, streptococci are predominant group.  Various species of Streptococci are encountered in mouth.  Most of these are alpha-haemolytic streptococci, collectively named as viridians streptococci
  • 9. Lactobacilli are frequently found in mouth. They are imp bacteria to promote the progress of carious lesion after the initiaton of dental caries by Strep mutans
  • 10.  Veilonella is the most common among Gram- negative bacteria.  Fusobacterium nucleatum is the most commonly occurring Gram-negative anaerobic bacillus  Other anaerobes such as Bacteroids, Leptotrichia, Selenomonas etc have also been found to be an imp group
  • 11. Oral treponema(T.vincenti,T.denticola,T.orale) are comman inhabitants of gingival crevice & sub gingival area  Due to their presence in subgingival plagues, they have been imp role in causing gingivitis and most forms of periodontitis.
  • 12. Besides bacteria normal flora of mouth may contain fungi and protozoa  FUNGI  Candida albicans  C.tropicalis  C.pseudotropicalis  C.stellatoidea  Cryptococcus sps  PROTOZOA  Entamoeba gingivalis  Trichomanas tenax
  • 13. Acquisition Of Normal Flora Of Mouth  The mouth of newborn at birth is usually microbiologically sterile.  Within few hours of birth Streptococci establish themselves in the mouth.
  • 14. Acquisition Of Normal Flora Of Mouth – Cont’d  Among Streptococci, Strep. salivarius is dominant at this stage.  During the first year of life other species of Streptococci,Staphylococci,Neisseriae,Veilonella, Actinomyces,Lactobacilli and Fusobacteria also colonize.
  • 15. Acquisition Of Normal Flora Of Mouth – Cont’d  With the eruption of teeth, solid surfaces in the oral cavity are available for colonization.  Strep mutans, Strep sanguis establish themselves on these hard surfaces.  Simultaneously, there is also increase in number of obligate anaerobes