1. The document provides instructions for using a virtual microscope tutorial. It guides the user through controls for lighting, selecting and mounting slides, adjusting objectives and focus, and making observations at different magnifications. 2. The user is asked to provide information about how different components work, such as controls for lighting and slide placement. They also record observations of different slides at 4x, 10x, 40x and 100x magnification. 3. Key steps covered include how to switch objectives lenses to achieve different magnifications, adjust focus both coarsely and finely, use controls to reposition the slide, and switch views between looking through and at the microscope.

1. Name: ____________________________________
www.bloust.com/cms7
Mrs. Henley
2nd
Quarter Notes … scroll down
(or go directly to http://www.udel.edu/biology/ketcham/microscope/)
Put your headphones on:
Virtual Microscope
1. The microscope will welcome you. Click the button to start the tour. Notice the volume level beneath it. Adjust the
level so that you can hear.
2. On the “Getting Started” list, Click the option: How to “Control Lighting”
a. What does the on/ off button do? __________________________________________________________________________________
b. There is a knob next to the on/ off button. You can turn it to level 1 all the way up to level 10. What does it
appear to do when you rotate it back and forth? ___________________________________________________________________
c. What does the light diaphragm do? __________________________________________________________________________________
d. Re-click the “Control Lighting” if you need to hear it again.
3. On the “How to” menu, select “Pick a slide”
a. What does the spring loaded clip do? ________________________________________________________________________________
b. What is a “slide”? _______________________________________________________________________________________________________
4. Click the letter “e” slide and watch it mount.
5. On the “Getting Started” list, Click the option: How to “Switch Objectives”
a. The blue lens magnifies ____ times closer
b. The green lens magnifies ____ times closer
c. The yellow lens magnifies ____ time closer
d. The red lens magnifies ____ times closer.
6. Total magnification: multiplying the lenses together in a compound microscope. The piece you look through is called
the EYEPIECE. The ocular lens in the eye piece magnifies objects 10 times closer. What is the total magnification of the
following?
a. The eye piece (10x) and the blue lens? ___________ times closer
b. The eye piece and the green lens? ___________ times closer
c. The eye piece and the yellow lens? ___________ times closer
d. The eye piece and the red lens? ___________ times closer
7. The nosepiece is the piece on the microscope that rotates and holds the ocular lenses (4x, 10x, 40x, and 100x). Move
the 4x ocular lens to the back where the light will shine through it.
8. On the “Getting Started” list, Click the option: How to “Reposition Slide Holder”
a. Follow directions for moving the specimen on the slide to be in the light.
9. On the “Getting Started” list, Click the option: How to “Adjust Focus”.
a. The coarse adjustment knob is the larger knob, and moves the stage _____________.
b. The fine focus knob moves it very ________.
c. Move the coarse adjustment knob to move the stage as __________ as it will go.
d. Click More
10. Click the “switch views” icon and follow directions.
a. You can use the “switch views” button to go from looking at the microscope to looking ______________ the
microscope.
11. On the “Getting Started” list, Click the option: How to “Adjust Oculars”.
a. Watch the demo. When you first look through a microscope the eye piece will not be adjusted to your eyes. It
is important that you understand how to move the eyepieces closer together or further apart. As you are
looking with both eyes, you should only see 1 circle. Adjust the oculars so that there is only 1 circle present.
12. On the “Getting Started” list, Click the option: How to “Adjust Focus”.

2. a. Always start in low power (4x) using the _______________ adjustment knob. You want to slowly move it down
until you see the speciman.
13. On the “Getting Started” list, Click the option: How to “Fine Tuning”.
a. Center your “e” in the middle of the line.
b. X/Y controls help to ________________________________________________________________________________________________
c. Notice: If you want your “e” to go to the left you would click the right or left xy control? ___________________
d. Fine adjustment knob helps _______________________________________________________________________________________
e. The diaphragm helps _______________________________________________________________________________________________
14. On the “Getting Started” list, Click the option: How to “Switch Objectives”. You may have to adjust the diaphram or
fine focus to see the “e” clearly after each switch. Practice switching your objectives by grabing the nosepiece and
rotating it in order of the following:
a. How much of the “e” do you see with the 4x? ____________________________________________________________________
b. How much of the “e” do you see with the 10x?____________________________________________________________________
c. How much of the “e” do you see with the 40x?_____________________________________________________________________
d. How much of the “e” do you see with the 100x?____________________________________________________________________
15. Switch back the 4x objective lens. Draw each of the slides listed at 4x, 10x, 40x, and 100x.
CHEEK SMEAR
4x 10x 40x 100x
ONION ROOT TIP
4x 10x 40x 100x
BACTERIAL CAPSULE
4x 10x 40x 100x
16. Why do you think the cells look differently? ________________________________________________________