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17 genetoprotein text
1. Chapter 17
From Gene to Protein
PowerPoint Lectures for
Biology, Seventh Edition
Neil Campbell and Jane Reece
Lectures by Chris Romero
Copyright Š 2005 Pearson Education, Inc. publishing as Benjamin Cummings
2. ⢠Overview: The Flow of Genetic Information
⢠The information content of DNA
â Is in the form of specific sequences of
nucleotides along the DNA strands
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3. ⢠The DNA inherited by an organism
â Leads to specific traits by dictating the
synthesis of proteins
⢠The process by which DNA directs protein
synthesis, gene expression
â Includes two stages, called transcription and
translation
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4. ⢠The ribosome
â Is part of the cellular machinery for translation,
polypeptide synthesis
Figure 17.1
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5. ⢠Concept 17.1: Genes specify proteins via
transcription and translation
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6. Evidence from the Study of Metabolic Defects
⢠In 1909, British physician Archibald Garrod
â Was the first to suggest that genes dictate
phenotypes through enzymes that catalyze
specific chemical reactions in the cell
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7. Nutritional Mutants in Neurospora: Scientific Inquiry
⢠Beadle and Tatum causes bread mold to
mutate with X-rays
â Creating mutants that could not survive on
minimal medium
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8. ⢠Using genetic crosses
â They determined that their mutants fell into three
classes, each mutated in a different gene
EXPERIMENT
RESULTS
Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring
arginine in their growth medium and had shown genetically that these mutants fell into three classes, each
defective in a different gene. From other considerations, they suspected that the metabolic pathway of arginine
biosynthesis included the precursors ornithine and citrulline. Their most famous experiment, shown here,
tested both their one geneâone enzyme hypothesis and their postulated arginine pathway. In this experiment,
they grew their three classes of mutants under the four different conditions shown in the Results section below.
The wild-type strain required only the minimal medium for growth. The three classes of mutants had
different growth requirements
Wild type
Minimal
medium
(MM)
(control)
MM +
Ornithine
MM +
Citrulline
Figure 17.2
MM +
Arginine
(control)
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Class I
Mutants
Class II
Mutants
Class III
Mutants
9. CONCLUSION
From the growth patterns of the mutants, Beadle and Tatum deduced that each mutant was unable
to carry out one step in the pathway for synthesizing arginine, presumably because it lacked the
necessary enzyme. Because each of their mutants was mutated in a single gene, they concluded that
each mutated gene must normally dictate the production of one enzyme. Their results supported the
one geneâone enzyme hypothesis and also confirmed the arginine pathway.
(Notice that a mutant can grow only if supplied with a compound made after the defective step.)
Wild type
Precursor
Gene A
Class I
Mutants
(mutation
in gene A)
Precursor
Precursor
Precursor
A
A
A
Ornithine
Ornithine
Ornithine
B
B
B
Citrulline
Citrulline
Citrulline
C
C
C
Arginine
Arginine
Arginine
Enzyme
A
Ornithine
Gene B
Enzyme
B
Citrulline
Gene C
Enzyme
C
Arginine
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Class II
Mutants
(mutation
in gene B)
Class III
Mutants
(mutation
in gene C)
10. ⢠Beadle and Tatum developed the âone geneâ
one enzyme hypothesisâ
â Which states that the function of a gene is to
dictate the production of a specific enzyme
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11. The Products of Gene Expression: A Developing Story
⢠As researchers learned more about proteins
â The made minor revision to the one geneâone
enzyme hypothesis
⢠Genes code for polypeptide chains or for RNA
molecules
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12. Basic Principles of Transcription and Translation
⢠Transcription
â Is the synthesis of RNA under the direction of
DNA
â Produces messenger RNA (mRNA)
⢠Translation
â Is the actual synthesis of a polypeptide, which
occurs under the direction of mRNA
â Occurs on ribosomes
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13. ⢠In prokaryotes
â Transcription and translation occur together
TRANSCRIPTION
DNA
mRNA
Ribosome
TRANSLATION
Polypeptide
(a) Prokaryotic cell. In a cell lacking a nucleus, mRNA
produced by transcription is immediately translated
without additional processing.
Figure 17.3a
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14. ⢠In eukaryotes
â RNA transcripts are modified before becoming
true mRNA
Nuclear
envelope
DNA
TRANSCRIPTION
Pre-mRNA
RNA PROCESSING
mRNA
Ribosome
TRANSLATION
Polypeptide
Figure 17.3b
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(b) Eukaryotic cell. The nucleus provides a separate
compartment for transcription. The original RNA
transcript, called pre-mRNA, is processed in various
ways before leaving the nucleus as mRNA.
15. ⢠Cells are governed by a cellular chain of
command
â DNA â RNA â protein
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16. The Genetic Code
⢠How many bases correspond to an amino
acid?
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17. Codons: Triplets of Bases
⢠Genetic information
â Is encoded as a sequence of nonoverlapping
base triplets, or codons
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18. ⢠During transcription
â The gene determines the sequence of bases
along the length of an mRNA molecule
Gene 2
DNA
molecule
Gene 1
Gene 3
DNA strand 3â˛
5â˛
A C C A A A C C G A G T
(template)
TRANSCRIPTION
mRNA
5â˛
U G G U U U G G C U C A
Codon
TRANSLATION
Protein
Figure 17.4
Trp
Amino acid
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Phe
Gly
Ser
3â˛
19. Cracking the Code
⢠A codon in messenger RNA
Figure 17.5
Second mRNA base
U
C
A
UAU
UUU
UCU
Tyr
Phe
UAC
UUC
UCC
U
UUA
UCA Ser UAA Stop
UAG Stop
UUG Leu UCG
CUU
CUC
C
CUA
CUG
CCU
CCC
Leu CCA
CCG
Pro
AUU
AUC
A
AUA
AUG
ACU
ACC
ACA
ACG
Thr
GUU
G GUC
GUA
GUG
lle
Met or
start
GCU
GCC
Val
GCA
GCG
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Ala
G
U
UGU
Cys
UGC
C
UGA Stop A
UGG Trp G
U
CAU
CGU
His
CAC
CGC
C
Arg
CAA
CGA
A
Gln
CAG
CGG
G
U
AAU
AGU
Asn
AAC
AGC Ser C
A
AAA
AGA
Lys
AAG
AGG Arg G
U
GAU
GGU
C
GAC Asp GGC
Gly
GAA
GGA
A
Glu GGG
GAG
G
Third mRNA base (3Ⲡend)
First mRNA base (5Ⲡend)
â Is either translated into an amino acid or serves as
a translational stop signal
20. ⢠Codons must be read in the correct reading
frame
â For the specified polypeptide to be produced
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21. Evolution of the Genetic Code
⢠The genetic code is nearly universal
â Shared by organisms from the simplest
bacteria to the most complex animals
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22. ⢠In laboratory experiments
â Genes can be transcribed and translated after
being transplanted from one species to
another
Figure 17.6
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23. ⢠Concept 17.2: Transcription is the DNAdirected synthesis of RNA: a closer look
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24. Molecular Components of Transcription
⢠RNA synthesis
â Is catalyzed by RNA polymerase, which pries
the DNA strands apart and hooks together the
RNA nucleotides
â Follows the same base-pairing rules as DNA,
except that in RNA, uracil substitutes for
thymine
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25. Synthesis of an RNA Transcript
⢠The stages of transcription are
Promoter
â Initiation
Transcription unit
5â˛
3â˛
3â˛
5â˛
Start point
RNA polymerase
â Elongation
â Termination
DNA
Initiation. After RNA polymerase binds to
the promoter, the DNA strands unwind, and
the polymerase initiates RNA synthesis at the
start point on the template strand.
1
5â˛
3â˛
Unwound
DNA
3â˛
5â˛
Template strand of
DNA
transcript
2 Elongation. The polymerase moves downstream, unwinding the
DNA and elongating the RNA transcript 5Ⲡâ 3 â˛. In the wake of
transcription, the DNA strands re-form a double helix.
Rewound
RNA
RNA
5â˛
3â˛
3â˛
5â˛
3â˛
5â˛
RNA
transcript
3 Termination. Eventually, the RNA
transcript is released, and the
polymerase detaches from the DNA.
5â˛
3â˛
3â˛
5â˛
5â˛
Figure 17.7
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Completed RNA
transcript
3â˛
26. Non-template
strand of DNA
Elongation
RNA nucleotides
RNA
polymerase
A
3â˛
T
C
C
C
A
A
T
U
3Ⲡend
G
T
A
U
C
A
T
E
A
G
G
C
A
G
T
A
A
T
Direction of transcription
(âdownstreamâ)
5â˛
Newly made
RNA
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G
5â˛
T
G
C
A
Template
strand of DNA
27. RNA Polymerase Binding and Initiation of Transcription
⢠Promoters signal the initiation of RNA synthesis
⢠Transcription factors
â Help eukaryotic RNA polymerase recognize
promoter sequences
1 Eukaryotic promoters
TRANSCRIPTION
DNA
RNA PROCESSING
Pre-mRNA
mRNA
TRANSLATION
Ribosome
Polypeptide
5â˛
3â˛
Promoter
3â˛
5â˛
T A T A A AA
AT A T T T T
TATA box
Start point
2
Template
DNA strand
Several transcription
factors
Transcription
factors
5â˛
3â˛
3 Additional transcription
3â˛
5â˛
factors
RNA polymerase II
5â˛
3â˛
Figure 17.8
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Transcription factors
3â˛
5â˛
5â˛
RNA transcript
Transcription initiation complex
28. Elongation of the RNA Strand
⢠As RNA polymerase moves along the DNA
â It continues to untwist the double helix,
exposing about 10 to 20 DNA bases at a time
for pairing with RNA nucleotides
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29. Termination of Transcription
⢠The mechanisms of termination
â Are different in prokaryotes and eukaryotes
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30. ⢠Concept 17.3: Eukaryotic cells modify RNA
after transcription
⢠Enzymes in the eukaryotic nucleus
â Modify pre-mRNA in specific ways before the
genetic messages are dispatched to the
cytoplasm
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31. Alteration of mRNA Ends
⢠Each end of a pre-mRNA molecule is modified
in a particular way
â The 5Ⲡend receives a modified nucleotide cap
â The 3Ⲡend gets a poly-A tail
A modified guanine nucleotide
added to the 5Ⲡend
TRANSCRIPTION
RNA PROCESSING
50 to 250 adenine nucleotides
added to the 3Ⲡend
DNA
Pre-mRNA
mRNA
5â˛
Protein-coding segment
3â˛
G P P P
AAUAAA
Ribosome
TRANSLATION
5ⲠCap
Polypeptide
Polyadenylation signal
5ⲠUTR
Start codon Stop codon
Figure 17.9
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3ⲠUTR
AAAâŚAAA
Poly-A tail
32. Split Genes and RNA Splicing
⢠RNA splicing
â Removes introns and joins exons
TRANSCRIPTION
RNA PROCESSING
DNA
Pre-mRNA
5ⲠExon Intron
Pre-mRNA 5ⲠCap
30
31
1
Coding
segment
mRNA
Ribosome
Intron
Exon
Exon
3â˛
Poly-A tail
104
105
146
Introns cut out and
exons spliced together
TRANSLATION
Polypeptide
mRNA 5ⲠCap
1
3ⲠUTR
Figure 17.10
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Poly-A tail
146
3ⲠUTR
33. ⢠Is carried out by spliceosomes in some cases
5â˛
1
RNA transcript (pre-mRNA)
Intron
Exon 1
Exon 2
Protein
Other proteins
snRNA
snRNPs
Spliceosome
2
5â˛
Spliceosome
components
3
Figure 17.11
5â˛
mRNA
Exon 1
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Exon 2
Cut-out
intron
34. Ribozymes
⢠Ribozymes
â Are catalytic RNA molecules that function as
enzymes and can splice RNA
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35. The Functional and Evolutionary Importance of Introns
⢠The presence of introns
â Allows for alternative RNA splicing
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36. ⢠Proteins often have a modular architecture
â Consisting of discrete structural and functional
regions called domains
⢠In many cases
â Different exons code for the different domains in a
protein
Gene
DNA
Exon 1 Intron Exon 2
Transcription
RNA processing
Intron Exon 3
Translation
Domain 3
Domain 2
Domain 1
Figure 17.12
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Polypeptide
37. ⢠Concept 17.4: Translation is the RNA-directed
synthesis of a polypeptide: a closer look
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38. Molecular Components of Translation
⢠A cell translates an mRNA message into
protein
â With the help of transfer RNA (tRNA)
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39. ⢠Translation: the basic concept
TRANSCRIPTION
DNA
mRNA
Ribosome
TRANSLATION
Polypeptide
Amino
acids
Polypeptide
Trp
Ph e
tRNA with
amino acid
Ribosome attached
Gly
tRNA
A
C
GC
C
A A A
U G G U U U G G C
Codons
5â˛
Figure 17.13
mRNA
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G
Anticodon
3â˛
40. ⢠Molecules of tRNA are not all identical
â Each carries a specific amino acid on one end
â Each has an anticodon on the other end
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41. The Structure and Function of Transfer RNA
⢠A tRNA molecule
A
â Consists of a single RNA strand C
that is only
C
about 80 nucleotides long
â Is roughly L-shaped
3â˛
A
C
C
A 5â˛
C G
G C
C G
U G
U A
A U
A U
U C
UA
C A C AG
*
G
*
G U G U *
C
C
* *
U C
*
* G AG C
(a) Two-dimensional structure. The four base-paired regions and
G C
U A
three loops are characteristic of all tRNAs, as is the base sequence
* G
of the amino acid attachment site at the 3Ⲡend. The anticodon triplet
A
A*
is unique to each tRNA type. (The asterisks mark bases that have
C
U
*
been chemically modified, a characteristic of tRNA.)
A
G
A
Figure 17.14a
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Amino acid
attachment site
Anticodon
C U C
G A G
A G *
*
G
A G G
Hydrogen
bonds
42. 5â˛
3â˛
Amino acid
attachment site
Hydrogen
bonds
A AG
3â˛
Anticodon
(b) Three-dimensional structure
Figure 17.14b
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5â˛
Anticodon
(c) Symbol used
in this book
43. ⢠A specific enzyme called an aminoacyl-tRNA
synthetase
â Joins each amino acid to the correct tRNA
Amino acid
P P
Aminoacyl-tRNA
synthetase (enzyme)
1 Active site binds the
amino acid and ATP.
P Adenosine
ATP
2 ATP loses two P groups
and joins amino acid as AMP.
P
Pyrophosphate
Pi
Phosphates
3 Appropriate
tRNA covalently
Bonds to amino
Acid, displacing
AMP.
P
Adenosine
Pi
Pi
tRNA
P Adenosine
AMP
4 Activated amino acid
is released by the enzyme.
Figure 17.15
Aminoacyl tRNA
(an âactivated
amino acidâ)
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44. Ribosomes
⢠Ribosomes
â Facilitate the specific coupling of tRNA
anticodons with mRNA codons during protein
synthesis
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45. ⢠The ribosomal subunits
â Are constructed of proteins and RNA
molecules named ribosomal RNA or rRNA
DNA
TRANSCRIPTION
mRNA
Ribosome
TRANSLATION
Polypeptide
Growing
polypeptide
Exit tunnel
tRNA
molecules
Large
subunit
E
P A
Small
subunit
5â˛
mRNA
Figure 17.16a
3â˛
(a) Computer model of functioning ribosome. This is a model of a bacterial
ribosome, showing its overall shape. The eukaryotic ribosome is roughly
similar. A ribosomal subunit is an aggregate of ribosomal RNA molecules
and proteins.
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46. ⢠The ribosome has three binding sites for tRNA
â The P site
â The A site
â The E site
P site (Peptidyl-tRNA
binding site)
A site (AminoacyltRNA binding site)
E site
(Exit site)
E
mRNA
binding site
Figure 17.16b
P
A
Large
subunit
Small
subunit
(b) Schematic model showing binding sites. A ribosome has an mRNA
binding site and three tRNA binding sites, known as the A, P, and E
sites. This schematic ribosome will appear in later diagrams.
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47. Amino end
Growing polypeptide
Next amino acid
to be added to
polypeptide chain
tRNA
3â˛
mRNA
5â˛
Codons
(c) Schematic model with mRNA and tRNA. A tRNA fits into a binding site when its anticodon
base-pairs with an mRNA codon. The P site holds the tRNA attached to the growing
polypeptide. The A site holds the tRNA carrying the next amino acid to be added to the
polypeptide chain. Discharged tRNA leaves via the E site.
Figure 17.16c
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48. Building a Polypeptide
⢠We can divide translation into three stages
â Initiation
â Elongation
â Termination
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49. Ribosome Association and Initiation of Translation
⢠The initiation stage of translation
â Brings together mRNA, tRNA bearing the first
amino acid of the polypeptide, and two
subunits of a ribosome
t
Me
P site
3ⲠU A C 5â˛
5ⲠA U G 3â˛
Initiator tRNA
t
Me
GTP
GDP
E
mRNA
5â˛
Start codon
mRNA binding site
Figure 17.17
3â˛
Small
ribosomal
subunit
1 A small ribosomal subunit binds to a molecule of
mRNA. In a prokaryotic cell, the mRNA binding site
on this subunit recognizes a specific nucleotide
sequence on the mRNA just upstream of the start
codon. An initiator tRNA, with the anticodon UAC,
base-pairs with the start codon, AUG. This tRNA
carries the amino acid methionine (Met).
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Large
ribosomal
subunit
5â˛
A
3â˛
Translation initiation complex
2 The arrival of a large ribosomal subunit completes
the initiation complex. Proteins called initiation
factors (not shown) are required to bring all the
translation components together. GTP provides
the energy for the assembly. The initiator tRNA is
in the P site; the A site is available to the tRNA
bearing the next amino acid.
50. Elongation of the Polypeptide Chain
⢠In the elongation stage of translation
â Amino acids are added one by one to the
preceding amino acid
TRANSCRIPTION
Amino end
of polypeptide
DNA
mRNA
Ribosome
TRANSLATION
Polypeptide
mRNA
Ribosome ready for
next aminoacyl tRNA
E
3â˛
P A
site site
5â˛
1 Codon recognition. The anticodon
of an incoming aminoacyl tRNA
base-pairs with the complementary
mRNA codon in the A site. Hydrolysis
of GTP increases the accuracy and
efficiency of this step.
2 GTP
2 GDP
E
E
P
Figure 17.18
3 Translocation. The ribosome
translocates the tRNA in the A
site to the P site. The empty tRNA
in the P site is moved to the E site,
where it is released. The mRNA
moves along with its bound tRNAs,
bringing the next codon to be
translated into the A site.
P
A
GDP
GTP
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E
P
A
A
2 Peptide bond formation. An
rRNA molecule of the large
subunit catalyzes the formation
of a peptide bond between the
new amino acid in the A site and
the carboxyl end of the growing
polypeptide in the P site. This step
attaches the polypeptide to the
tRNA in the A site.
51. Termination of Translation
⢠The final stage of translation is termination
â When the ribosome reaches a stop codon in
the mRNA
Release
factor
Free
polypeptide
5â˛
3â˛
3â˛
5â˛
5â˛
3â˛
Stop codon
(UAG, UAA, or UGA)
1 When a ribosome reaches a stop 2 The release factor hydrolyzes 3 The two ribosomal subunits
codon on mRNA, the A site of the the bond between the tRNA in and the other components of
ribosome accepts a protein called the P site and the last amino
the assembly dissociate.
a release factor instead of tRNA.
acid of the polypeptide chain.
The polypeptide is thus freed
from the ribosome.
Figure 17.19
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52. Polyribosomes
⢠A number of ribosomes can translate a single
mRNA molecule simultaneously
â Forming a polyribosome
Completed
polypeptide
Growing
polypeptides
Incoming
ribosomal
subunits
Start of
mRNA
(5Ⲡend)
Polyribosom
e
End of
mRNA
(3Ⲡend)
(a) An mRNA molecule is generally translated simultaneously
by several ribosomes in clusters called polyribosomes.
Ribosomes
mRNA
0.1 Âľm
Figure 17.20a, b
(b) This micrograph shows a large polyribosome in a prokaryotic
cell (TEM).
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53. Completing and Targeting the Functional Protein
⢠Polypeptide chains
â Undergo modifications after the translation
process
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54. Protein Folding and Post-Translational Modifications
⢠After translation
â Proteins may be modified in ways that affect
their three-dimensional shape
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55. Targeting Polypeptides to Specific Locations
⢠Two populations of ribosomes are evident in
cells
â Free and bound
⢠Free ribosomes in the cytosol
â Initiate the synthesis of all proteins
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56. ⢠Proteins destined for the endomembrane
system or for secretion
â Must be transported into the ER
â Have signal peptides to which a signalrecognition particle (SRP) binds, enabling the
translation ribosome to bind to the ER
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57. ⢠The signal mechanism for targeting proteins to
the ER
1 Polypeptide
synthesis begins
on a free
ribosome in
the cytosol.
2 An SRP binds
to the signal
peptide, halting
synthesis
momentarily.
3 The SRP binds to a
receptor protein in the ER
membrane. This receptor
is part of a protein complex
(a translocation complex)
that has a membrane pore
and a signal-cleaving enzyme.
4 The SRP leaves, and
the polypeptide resumes
growing, meanwhile
translocating across the
membrane. (The signal
peptide stays attached
to the membrane.)
5 The signalcleaving
enzyme
cuts off the
signal peptide.
6 The rest of
the completed
polypeptide leaves
the ribosome and
folds into its final
conformation.
Ribosome
mRNA
Signal
peptide
Signalrecognition
particle
(SRP) SRP
receptor
CYTOSOL protein
ERLUMEN
Translocation
complex
Figure 17.21
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Signal
peptide
removed
ER
membrane
Protein
58. ⢠Concept 17.5: RNA plays multiple roles in the
cell: a review
⢠RNA
â Can hydrogen-bond to other nucleic acid
molecules
â Can assume a specific three-dimensional
shape
â Has functional groups that allow it to act as a
catalyst
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59. ⢠Types of RNA in a Eukaryotic Cell
Table 17.1
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60. ⢠Concept 17.6: Comparing gene expression in
prokaryotes and eukaryotes reveals key differences
⢠Prokaryotic cells lack a nuclear envelope
â Allowing translation to begin while transcription is
still in progress
RNA polymerase
DNA
mRNA
Polyribosome
RNA
polymerase
Direction of
transcription
DNA
Polyribosome
Polypeptide
(amino end)
Ribosome
Figure 17.22
0.25 Âľm
mRNA (5Ⲡend)
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61. ⢠In a eukaryotic cell
â The nuclear envelope separates transcription
from translation
â Extensive RNA processing occurs in the
nucleus
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62. ⢠Concept 17.7: Point mutations can affect
protein structure and function
⢠Mutations
â Are changes in the genetic material of a cell
⢠Point mutations
â Are changes in just one base pair of a gene
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63. ⢠The change of a single nucleotide in the DNAâs
template strand
â Leads to the production of an abnormal protein
Wild-type hemoglobin DNA
3â˛
Mutant hemoglobin DNA
C T
T
G U A
5â˛
In the DNA, the
mutant template
strand has an A where
the wild-type template
has a T.
The mutant mRNA has
a U instead of an A in
one codon.
3â˛
5â˛
T
C A
mRNA
mRNA
G A
A
5â˛
3â˛
5â˛
3â˛
Normal hemoglobin
Sickle-cell hemoglobin
Glu
Val
Figure 17.23
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The mutant (sickle-cell)
hemoglobin has a valine
(Val) instead of a glutamic
acid (Glu).
64. Types of Point Mutations
⢠Point mutations within a gene can be divided
into two general categories
â Base-pair substitutions
â Base-pair insertions or deletions
Copyright Š 2005 Pearson Education, Inc. publishing as Benjamin Cummings
65. Substitutions
⢠A base-pair substitution
â Is the replacement of one nucleotide and its
partner with another pair of nucleotides
â Can cause missense or nonsense
Wild type
mRNA
Protein
5â˛
A U G
Met
A A G U U U GG C U A A
Lys
Phe
Gly
3â˛
Stop
Amino end
Carboxyl end
Base-pair substitution
No effect on amino acid sequence
U instead of C
A U G A A G U U U G G U U A A
Met
Lys
Missense
Phe
Gly
Stop
A instead of G
A U G A A G U U U A G U U A A
Met
Lys
Phe
Ser
Stop
Nonsense
U instead of A
A U G U A G U U U G G C U A A
Figure 17.24
Met
Stop
Copyright Š 2005 Pearson Education, Inc. publishing as Benjamin Cummings
66. Insertions and Deletions
⢠Insertions and deletions
â Are additions or losses of nucleotide pairs in a
gene
â May produce frameshift mutations
Wild type
mRNA
Protein
5â˛
A UG A A GU U U G G C U A A
Met
Lys
Gly
Phe
Stop
Amino end
Carboxyl end
Base-pair insertion or deletion
Frameshift causing immediate nonsense
Extra U
AU G U A AG U U U G GC U A
Met
Stop
Frameshift causing
extensive missense
U Missing
A U G A A GU U G G C U A A
Met
Lys
Leu
Ala
Insertion or deletion of 3 nucleotides:
no frameshift but extra or missing amino acid
A AG
Missing
A U G U U U G G C U A A
Figure 17.25
Met
Phe
Gly
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Stop
3â˛
67. Mutagens
⢠Spontaneous mutations
â Can occur during DNA replication,
recombination, or repair
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68. ⢠Mutagens
â Are physical or chemical agents that can
cause mutations
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69. What is a gene? revisiting the question
⢠A gene
â Is a region of DNA whose final product is either
a polypeptide or an RNA molecule
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70. ⢠A summary of transcription and translation in a
eukaryotic cell
DNA
TRANSCRIPTION
1 RNA is transcribed
from a DNA template.
3â˛
5â˛
RNA
transcript
A
lyPo
RNA
polymerase
RNA PROCESSING
Exon
2 In eukaryotes, the
RNA transcript (premRNA) is spliced and
modified to produce
mRNA, which moves
from the nucleus to the
cytoplasm.
RNA transcript
(pre-mRNA)
Intron
Aminoacyl-tRNA
synthetase
p
Ca
NUCLEUS
Amino
acid
tRNA
FORMATION OF
INITIATION COMPLEX
CYTOPLASM 3 After leaving the
nucleus, mRNA attaches
to the ribosome.
AMINO ACID ACTIVATION
4
Each amino acid
attaches to its proper tRNA
with the help of a specific
enzyme and ATP.
Growing
polypeptide
mRNA
A
lyPo
Activated
amino acid
Ribosomal
subunits
p
Ca
5â˛
TRANSLATION
C
AC
U
A AC
E
AAA
UG GUU U A U G
Codon
Figure 17.26
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Ribosome
A succession of tRNAs
add their amino acids to
the polypeptide chain
Anticodon
as the mRNA is moved
through the ribosome
one codon at a time.
(When completed, the
polypeptide is released
from the ribosome.)
5
A
lyPo